Liquid-based thin-layer cell preservation solution and use thereof
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A thin-layer cell and treatment solution technology, applied in the field of cell treatment solution, can solve the problems of expensive cell preservation treatment solution, high detection fee, and difficulty in low-income areas, and achieve good cell staining contrast, which is conducive to application and promotion. The effect of clear microscope inspection field of view
Active Publication Date: 2010-12-15
山东龙奥生物技术有限公司
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Problems solved by technology
[0004] At present, the imported cell preservation treatment solution is expensive, and the cost of a liquid-based thin-layer cell inspection is about 180 yuan. Such a high inspection fee makes it difficult to promote this technology in low-income areas; among similar domestic products, such a...
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Embodiment 1
[0025] Weigh or measure each component according to the table below,
[0026] Formaldehyde solution with mass concentration of 25% 50mL
[0027] Mass concentration of 36% hydrochloric acid solution 1mL
[0028] Potassium chloride 1g
[0029] Tris hydroxymethyl amino methane 1g
[0030] Double distilled water 949mL
[0031] The components are mixed and then stirred and dissolved to obtain the preservation treatment solution.
Embodiment 2
[0033] Weigh or measure each component according to the table below,
[0034] 280mL glutaraldehyde solution with a mass concentration of 30%
[0035] Mass concentration of 36% hydrochloric acid solution 30mL
[0036] Sodium chloride 60g
[0037] Tris 30g
[0038] Double distilled water 690mL
[0039] The components are mixed and then stirred and dissolved to obtain the preservation treatment solution.
Embodiment 3
[0041] Weigh or measure each component according to the table below,
[0042] The mass concentration is 35% paraformaldehyde solution 100mL
[0043] Glacial acetic acid 20mL
[0044] Calcium chloride 10g
[0045] Tris 10g
[0046] Double distilled water 880mL
[0047] The components are mixed and then stirred and dissolved to obtain the preservation treatment solution.
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Abstract
The invention discloses liquid-based thin-layer cell preservation solution and use thereof. The preservation solution comprises 5 to 28 volume percent of aldehyde solution, 0.1 to 3 volume percent of acid solution, 0.1 to 6 mass volume percent of chloride, 0.1 to 3 mass volume percent of tris(hydroxymethyl)aminomethane and the balance volume percent of double distilled water. The preservation solution of the invention can remove red blood cells, preserve white blood cells, timely fix the original state of cells of diagnostic value, soften mucilage and epidermal tissues, weaken mucous effect and separate effective cells from mucilage. The microscopic examination piece manufactured by using the preservation solution has clear microscopic examination view field and high cellular staining contrast and is widely suitable for gynecological and non-gynecological exfoliative cytological specimens and manual, semi-automatic or fully-automatic liquid-based cell substrate making processes. The production cost of the preservation solution is low, so the application and promotion of a liquid-based thin-layer cell detection technique are convenient.
Description
technical field [0001] The invention relates to a cell treatment liquid, in particular to a liquid-based thin-layer cell preservation treatment liquid and its application. Background technique [0002] The commonly used preparation method in cell detection is the Pap cell smear method. The doctor needs to manually smear the cell sample obtained with the collector on the microscope. The smear made by this traditional manual method only collects a maximum of 20% of the cells, while more than 80% of the cell samples remain on the sampler and are discarded together with the sampler. In addition, more than 40% of the smears will become cloudy due to the influence of blood, mucus and inflammatory tissue, as well as artifacts caused by untimely fixation. These shortcomings are responsible for the inaccurate results of most routine Pap tests. [0003] The liquid-based thin-layer cytology method is an internationally advanced cytological examination technology developed in recent y...
Claims
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Application Information
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