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Blood cell analyzer calibrator and preparation process thereof

A blood cell analyzer and preparation process technology, applied in the preparation of test samples, material inspection products, biological tests, etc., can solve problems such as inability to use calibration, and achieve easy mixing, low viscosity, and no matrix effect. Effect

Inactive Publication Date: 2010-03-17
TECOM SCI CORP
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AI Technical Summary

Problems solved by technology

A certain type of calibrator can only be used to calibrate instruments of the same type, and cannot be used to calibrate instruments of other brands

Method used

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Embodiment Construction

[0023] Cell activity protection solution and stabilizer of the present invention include protection solution (agent), Wb 10 and Wb 22 liquid.

[0024] Formula composition

[0025] (1) Whole blood cell activity protection solution (Rb solution): sodium chloride 5.0g / L, citric acid 0.2g / L, sodium citrate 2.0g / L, sodium dihydrogen phosphate 0.75g / L, adenine 0.3 g / L, glucose 7.5g / L, mannitol 10.0g / L, trehalose 7.8g / L, D-mannose 0.6g / L, glycerin 8.0g / L, 1.2-propanediol 0.4g / L, EDTA0 .25g / L, appropriate amount of biological antibacterial agent. Store in refrigerator at 2-8°C for later use.

[0026] (2) whole blood cell stabilizer I (Wb 10 solution): Rb solution 100.0 mL, dimethyl sulfoxide 0.5 mL, formaldehyde 3.0 mL, glutaraldehyde 0.05 mL, sodium azide 0.15 g. Store in refrigerator at 2-8°C for later use.

[0027] (3) whole blood cell stabilizer II (Wb 22 solution): Rb solution 100.0mL, formaldehyde 1.0mL, paraformaldehyde 0.25g, acetaldehyde 4.5mL, sodium azide 0.2g. Sto...

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Abstract

The invention uses synergetic combination effect of multiple cytoactive protection solution and a stabilizer, and a protective agent provides nutrition components and living environment required by maintaining normal physiological activity for blood cells for anaerobic glycolysis; the stabilizer containing cytolemma and cytoplasm fixed substances with lower concentration can slowly permeate the cytolemma to act on the cytoplasm and firmly combine with biomolecule of cell components such as protein, enzyme, lipid and the like effectively, thus enabling white blood cells, red blood cells and blood platelets to be fixed in different degrees and get effective protection and enabling blood cells to keep apparent morphological characteristics thereof. The blood cells in protection and good stabilized treatment is subjected to assignment according to traceability link delivery procedures and reference methods thereof, and can be used as blood cell analyzer calibrators for clinic usage. The invention uses stem complete blood cell (CBC) from human beings or animals, thus having good stability; the invention can be used in microscope manual counting and cell staining analysis, and has longerexpirydate compared with the current full blood cell calibrators.

Description

technical field [0001] The invention relates to the preparation of blood cell biological activity protection solution and stabilizer and the preparation technology of calibrator (material) for blood cell analyzer. Background technique [0002] In the process of quality control of hematology analyzers, instrument calibration or correction is essential, and instrument calibration should be performed after new instrument installation or each repair. Calibrators for hematology analyzers are standard substances recognized by authoritative departments. A certain type of calibrator can only be used to calibrate instruments of the same type, and cannot be used to calibrate instruments of other brands. The accuracy of the results and the comparability of the test results of different laboratories can only be guaranteed if the test results of the whole blood cell analysis are directly or indirectly traceable to the reference method. Whole blood cell calibrator is an essential measur...

Claims

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Application Information

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IPC IPC(8): G01N33/96G01N1/38
Inventor 颜箫颜菲
Owner TECOM SCI CORP
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