The invention provides a group of thyroid cancer markers and an application thereof. The markers comprise thyroid cancer molecular typing related genes, targeted medication related genes, chemotherapymedication related genes, operation prompt related genes, prognosis related genes and thyroid cancer heredity related genes. According to the invention, aiming at all exon regions of 44 genes relatedto thyroid molecular typing, targeted medication, chemotherapy medication, operation prompt, prognosis and heredity, high-depth sequencing is carried out; meanwhile, various variation types like SNV / Indel and gene fusion of genes are analyzed; and molecular level information of thyroid nodules and thyroid cancer is deeply analyzed. Aiming at a capture probe designed for the 44 genes, 558 targeting target regions are covered; the probe comprises 3633 sequences with a total size of 254.096 Kbp; and a prepared kit is wide in coverage, high in cost performance and high in effectiveness, can provide a reference basis for further molecular typing, medication prompt, genetic risk assessment and the like of a thyroid patient, and is applicable to clinical popularization and application.