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Thyroid nodule benign and malignant related marker and application thereof

A technology for thyroid nodules and markers, applied in the field of markers related to benign and malignant thyroid nodules, can solve problems such as abnormal thyroid function and clinical evaluation deviation, and achieve the effect of simple operation, low equipment requirements, and convenient promotion and application

Active Publication Date: 2021-07-30
SINGLERA GENOMICS (SHANGHAI) LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nodules detected by ultrasound are usually not associated with abnormal thyroid function, and the misuse of high-resolution thyroid ultrasound has led to the detection of a large number of clinically insignificant nodules, including thyroid microcarcinomas, which may bias the clinical assessment of thyroid dysfunction

Method used

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  • Thyroid nodule benign and malignant related marker and application thereof
  • Thyroid nodule benign and malignant related marker and application thereof
  • Thyroid nodule benign and malignant related marker and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0229] Example 1: TTC34 gene, RCOR2 gene, SLC16A3 gene and RARG gene combined for discrimination of benign and malignant thyroid nodules

[0230] Methylation-specific PCR was performed on the plasma cfDNA of 196 patients with thyroid cancer and 148 patients with benign thyroid nodules. There are differences in the level of kylation, the results are as follows figure 1 shown.

[0231] 1.1 Sample preparation

[0232] cfDNA was extracted from the plasma of 196 cases of thyroid cancer and 148 cases of benign thyroid nodules using QIAamp DNA Mini Kit (QIAGEN, Cat. No.: 51304); using Qubit TM dsDNA HS Assay Kit (Thermo, catalog number: Q32854) was used to detect the concentration of DNA; 1% agarose gel electrophoresis was used for quality inspection.

[0233] 1.2 DNA Transformation

[0234] Use MethylCode TM Bisulfite Conversion Kit (Thermo, catalog number: MECOV50) was used to perform bisulfite conversion on the DNA obtained in step 1, and unmethylated cytosine (cytosine, C)...

Embodiment 2

[0245] Example 2: Combination of TTC34 gene, ITPKA gene, SLC16A3 gene and RARG gene for discrimination of benign and malignant thyroid nodules

[0246] The applicant performed methylation-specific PCR on the plasma cfDNA of 196 patients with thyroid cancer and 148 patients with benign thyroid nodules, and found that TTC34 gene, ITPKA gene, SLC16A3 gene and RARG gene were significantly different in the tissue DNA of thyroid cancer and benign thyroid nodules cases. There are differences in the methylation levels of , the results are as follows figure 2 shown.

[0247] 2.1 Sample preparation

[0248] cfDNA was extracted from the plasma of 196 cases of thyroid cancer and 148 cases of benign thyroid nodules using QIAamp DNA Mini Kit (QIAGEN, Cat. No.: 51304); using Qubit TM dsDNA HS Assay Kit (Thermo, catalog number: Q32854) was used to detect the concentration of DNA; 1% agarose gel electrophoresis was used for quality inspection.

[0249] 2.2 DNA Transformation

[0250] Use...

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Abstract

The invention relates to a thyroid nodule benign and malignant related marker and application thereof. The marker is the following target markers: a TTC34 gene or a TTC34 sequence of a genome, an SLC16A3 gene or an SLC16A3 sequence of the genome, an RARG gene or an RARG sequence of the genome, and any one or all of two selected from an RCOR2 sequence of an RCOR2 gene or a genome and an ITPKA sequence of an ITPKA gene or a genome. The invention relates to application of primer molecules and / or probe molecules hybridized with the target marker in preparation of a detection reagent or a diagnostic kit for diagnosing benign and malignant thyroid nodules, and application of a device for determining the methylation level of the target marker in preparation of the diagnostic kit for diagnosing benign and malignant thyroid nodules.

Description

technical field [0001] The invention belongs to the field of molecular auxiliary diagnosis, and relates to markers related to benign and malignant thyroid nodules and applications thereof. Background technique [0002] DNA methylation is a mechanism of epigenetic inheritance, a common epigenetic modification of the genome of eukaryotic cells, which can change genetic expression without changing the DNA sequence. The so-called DNA methylation refers to the covalent binding of a methyl group at the 5th carbon position of cytosine of a genomic CpG dinucleotide under the action of DNA methyltransferase. DNA methylation plays an important role in cell proliferation, differentiation, and development, and is closely related to the occurrence and development of tumors. Its effects include transcriptional inhibition, chromatin structure regulation, X chromosome inactivation, and genomic imprinting. Abnormal DNA methylation can participate in tumorigenesis and progression by affectin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12Q1/6886C12Q1/6851
CPCC12Q1/6851C12Q1/6883C12Q1/6886C12Q2600/154C12Q2600/166C12Q2531/113C12Q2545/101C12Q2537/143
Inventor 苏明扬杨世方马建华郗大勇何其晔刘蕊
Owner SINGLERA GENOMICS (SHANGHAI) LTD
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