Flocculating bacterium basophilic halomonas NCSL-JXB2 as well as culture method and application of flocculating bacterium basophilic halomonas NCSL-JXB2

Abstract

The invention discloses a Halomonas alkaliphila NCSL-JXB2 which is a flocculating bacterium, and the preservation number of the Halomonas alkaliphila NCSL-JXB2 is CGMCC (China General Microbiological Culture Collection Center) No.33827. The invention further discloses a preparation method of the Halomonas alkaliphila NCSL-JXB2. The invention also discloses a culture method and application of the basophilic halomonas. The flocculating bacterium Halomonas alkaliphila NCSL-JXB2 disclosed by the invention can tolerate an ultra-high salt environment, is wide in salt tolerance range, can survive in an environment with the salinity of 0-25wt%, and has a good flocculation effect, and the self-flocculation rate is 66.3%. The strain can be used as a biological enhancement means to assist other biological processes, or is added together with other functional strains, or is added independently to accelerate the process starting speed, improve the operation effect of the biological process, improve the treatment efficiency of high-salt organic wastewater and reduce the operation cost.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of biological wastewater treatment, and particularly relates to a flocculating bacterium Halomonas alkaliphila NCSL-JXB2 tolerable to an ultrahigh-salt environment, a culture method of the flocculating bacterium, and application of the flocculating bacterium. BACKGROUND

[0002] The discharge amount of high-salt wastewater is increasing year by year, and the environmental protection requirement is increasing day by day, and the treatment and disposal thereof is still an industry problem. The physical and chemical method has high treatment cost and is easy to cause secondary pollution, and compared with the biological method, the biological method is environmentally friendly and low in cost. However, due to the high salt content and water quality fluctuation of the actual high-salt wastewater, the biological process often has problems such as long start-up time, unstable operation, and system collapse. In terms of specific process, the activated sludge process is more prone to sludge bulking and sludge loss in a high-salt system, the biofilm process is prone to slow start-up, sludge loss and other problems in a high-salt system, and the membrane bioreactor is more prone to membrane blockage and membrane pollution in a high-salt system.

[0003] The self-flocculating bacterium can adsorb and aggregate microorganisms by using the characteristics of its own flagellum winding, secreting a large amount of extracellular polymer or neutralizing surface charge, and the like, and can be used as a biological strengthening means to assist other biological processes, or can be co-added with other functional strains, or can be added alone to accelerate the start-up speed of the biological process, improve the operation effect of the biological process, improve the treatment efficiency of high-salt organic wastewater, and reduce the overall operation cost.

[0004] At present, flocculating bacteria such as Peseudomonas guguanensis, Bacillus licheniformis, Psychrobacter aquimaris and Pseudomonas hibiscicola are reported. In terms of Halomonas alkaliphila, it has been used for organic matter degradation and nitrogen and sulfur removal in salt-containing wastewater, and is reported to have salt tolerance, heterotrophic nitrification, aerobic denitrification and sulfur oxidation functions, and there is no report on the flocculation function of Halomonas alkaliphila. SUMMARY

[0005] In order to solve the problems of long start-up time and unstable operation of the biological process in the treatment of high-salt wastewater, and to fill the research gap in the function of microbial flocculation, the present application provides a flocculating bacterium Halomonas alkaliphila NCSL-JXB2 tolerable to an ultrahigh-salt environment, a culture method of the flocculating bacterium, and application of the flocculating bacterium.

[0006] In a first aspect, the present invention provides a strain of flocculating bacterium Halomonas alkaliphila NCSL-JXB2, which has accession number CGMCC No. 33827.

[0007] Preferably, the nucleotide sequence of the 16S rDNA of the alkalophilic halophila NCSL-JXB2 is shown in SEQ ID NO.1.

[0008] A second aspect of the present invention provides a method for culturing the flocculant bacterium *Halomonas alkaliphila* NCSL-JXB2, comprising the following steps:

[0009] Step 1: Inoculate the strain into a solid culture medium and activate it at 30°C for 2–6 days;

[0010] Step 2: Transfer single colonies to liquid culture medium and amplify them at 30°C for 1–4 days;

[0011] The liquid culture medium consists of: carbon source 1–100 g / L, ammonium chloride 0.2–20 g / L, potassium dihydrogen phosphate 0.05–8 g / L, magnesium sulfate heptahydrate 0.01–5 g / L, calcium chloride 0.01–5 g / L, ferrous sulfate heptahydrate 0.01–5 g / L, yeast extract 0.1–5 g / L, and sodium chloride 0–250 g / L.

[0012] The carbon source is selected from one or more combinations of glucose, sucrose, starch, sodium acetate, glycerol, and molasses;

[0013] The solid culture medium in step 1 consists of the liquid culture medium from step 2 plus 15-25 g / L agar.

[0014] A third aspect of the invention provides the application of the aforementioned flocculating bacterium, *Halomonas alkaliphila* NCSL-JXB2, comprising:

[0015] (1) As a co-addition of flocculants and activated sludge, it is used to assist and enhance the biological process for treating high-salt organic wastewater.

[0016] (2) When added in combination with other functional strains, it is used to improve the operational efficiency of biological processes for treating high-salt organic wastewater; and

[0017] (3) Add separately for the treatment of high-salt organic wastewater.

[0018] According to a preferred embodiment of the present invention, the biological process for assisting in the treatment of high-salt organic wastewater is selected from: assisting in the treatment of suspended activated sludge, assisting in the treatment of attached biofilm, and assisting in the treatment of activated sludge granulation.

[0019] According to a preferred embodiment of the present invention, the method of co-addition with other functional strains to improve the operational efficiency of the biological process for treating high-salt organic wastewater is selected from:

[0020] It works in conjunction with phenol-degrading bacteria to form phenol-degrading granular sludge, with pyridine-degrading bacteria to form pyridine-degrading granular sludge, with Penicillium fimbriae mycelial balls to rapidly form ultra-high salt-tolerant granular sludge, and with nitrifying bacteria to form highly efficient simultaneous nitrification and denitrification (SND) granular sludge.

[0021] According to a preferred embodiment of the present invention, the high-salt organic wastewater is selected from one or more of the following: simulated wastewater, pickled mustard tuber wastewater, soy sauce brewing wastewater, fish pickling wastewater, seafood aquaculture wastewater, oil and gas wastewater, pharmaceutical wastewater, textile printing and dyeing wastewater, chemical wastewater, reverse osmosis concentrate, and electrodialysis concentrate.

[0022] The main pollutants in the high-salt organic wastewater are organic matter, nitrogen, and phosphate.

[0023] The salinity of the high-salt organic wastewater is expressed as sodium chloride, with a sodium chloride concentration range of 30–250 g / L; or the salinity of the high-salt organic wastewater is expressed as conductivity, with a conductivity range of 50–500 mS / cm; or the salinity of the high-salt organic wastewater is expressed as total dissolved solids, with a total dissolved solids content range of 30–330 g / L.

[0024] Preferably, the application of the flocculant *Halomonas alkaliphila* NCSL-JXB2 includes centrifuging the bacterial culture of *Halomonas alkaliphila* NCSL-JXB2 cultured according to the aforementioned method, collecting the bacterial cells, and adding them to the bioreactor at a bacterial cell concentration of 0.01–5 g / L to improve the operating effect of the bioprocess.

[0025] The present invention has the following beneficial effects:

[0026] This invention provides a strain of alkalophilic halophilic bacterium, Halomonas alkaliphila NCSL-JXB2, which can tolerate ultra-high salinity environments. It has a wide salt tolerance range, surviving in salinity environments of 0–25 wt%, and exhibits excellent flocculation performance with a self-flocculation rate of 66.3%. This strain can be used as a bioaugmentation tool to assist other biological processes, or added in combination with other functional strains, or added alone to accelerate process start-up, improve the operational efficiency of biological processes, enhance the treatment efficiency of high-salt organic wastewater, and reduce operating costs. Attached Figure Description

[0027] Figure 1 The morphology of Halomonas alkaliphila NCSL-JXB2 was shown on a plate containing 90 g / L sodium chloride.

[0028] Figure 2 The morphology of Halomonas alkaliphila NCSL-JXB2 was shown on plates containing 10 g / L sodium chloride, 100 g / L sodium chloride, 120 g / L sodium chloride, 150 g / L sodium chloride, and 250 g / L sodium chloride.

[0029] Figure 3 Phylogenetic tree of Halomonas alkaliphila NCSL-JXB2.

[0030] Depositing matters

[0031] The flocculant strain (salinity 0-25 wt%) obtained in this invention, which can tolerate ultra-high salinity environments, is Halomonas alkaliphila NCSL-JXB2, which was deposited at the China General Microbiological Culture Collection Center (CGMCC) on March 14, 2025, with accession number CGMCC No. 33827. Detailed Implementation

[0032] The present invention will be further described in detail below through specific embodiments. It should be understood that the following embodiments are for illustrative purposes only and are not intended to limit the scope of the invention.

[0033] Unless otherwise specified, the experimental materials, operating methods, etc. used in the following embodiments can be obtained through conventional commercial channels, are known in the technical field, or are performed in accordance with the product instructions.

[0034] In the context of this invention, the salinity of the high-salinity organic wastewater is expressed as sodium chloride, and the concentration of sodium chloride ranges from 30 to 250 g / L; or the salinity of the high-salinity organic wastewater is expressed as conductivity, and the conductivity ranges from 50 to 500 mS / cm; or the salinity of the high-salinity organic wastewater is expressed as total dissolved solids (TDS), and the total dissolved solids content ranges from 30 to 330 g / L.

[0035] The alkalophilic halophila (Halomonas alkaliphila) NCSL-JXB2 obtained in this invention, after being cultured on a solid medium containing 90 g / L sodium chloride, produced colonies that were round with regular edges, yellowish with a slight pink tinge. Figure 1 As shown. This strain has been submitted to the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCCNo.33827.

[0036] Figure 2 The growth of the alkaliphila NCSL-JXB2 strain of this invention was shown on plates with five different salinities (10 g / L sodium chloride, 100 g / L sodium chloride, 120 g / L sodium chloride, 150 g / L sodium chloride, and 250 g / L sodium chloride). It can be seen that this strain can grow in all five salinity systems, exhibiting a wide salt tolerance range, and its growth rate is only slightly affected by increasing salinity.

[0037] Figure 3 Phylogenetic tree of Halomonas alkaliphila NCSL-JXB2.

[0038] The nucleotide sequence of the 16S rDNA of *Halomonas alkaliphila* NCSL-JXB2 of the present invention preferably includes the sequence shown in SEQ ID NO.1, specifically as follows:

[0039]

[0040] The screening, isolation, and self-flocculation rate determination method for Halomonas alkaliphila NCSL-JXB2 of the present invention is as follows:

[0041] 1. Culture medium:

[0042] 9% salinity LB solid medium: tryptone 10 g / L, yeast extract 5 g / L, sodium chloride 90 g / L, agar 18 g / L.

[0043] LB liquid medium: tryptone 10 g / L, yeast extract 5 g / L, sodium chloride 10 g / L.

[0044] 2. Experimental steps:

[0045] S1. Crush the mature granular sludge, dip a small amount of sludge into an LB solid plate, streak it on a plate, and incubate it in a 30℃ constant temperature incubator for 2-6 days.

[0046] S2. Take a small amount of the grown bacterial single colony and streak it three times on an LB solid plate.

[0047] S3. Pick a single colony and inoculate it into LB liquid medium. Incubate at 30°C and 160 rpm in a constant temperature shaker for 1 day. Take the fermentation broth of the strain and divide it into 10... -1 10 -3 10 -5 10 -7 Dilute the bacterial solution according to the specified ratio, spread the diluted bacterial solution onto LB agar plates, and incubate at 30°C for 2–6 days.

[0048] S4. Pick a single colony from the plate and inoculate it into LB liquid medium. Incubate at 30℃ and 160 rpm in a constant temperature shaker for 1 day. After mixing the bacterial solution, pour it into a test tube, take 1 mL and measure the absorbance at 550 nm, and record it as A0. After standing for 30 minutes, take 1 mL of the supernatant and measure the absorbance at 550 nm, and record it as A1.

[0049] Self-flocculation rate (%) = (A0 - A1) ÷ A0 × 100%.

[0050] The self-flocculation rate of the alkalophilic halophilic bacterium NCSL-JXB2 of the present invention was measured to be good, reaching 66.3%.

[0051] Example 1: Cultivation of Alkalophilic Halomonas NCSL-JXB2 and its role in enhancing the suspended activated sludge process

[0052] This embodiment includes the following steps:

[0053] Step 1: Take the bacterial culture from the preservation tube containing this strain and streak it on a solid culture medium plate for isolation and activation culture. The activation culture conditions are 30℃ and 2 days.

[0054] The solid culture medium consists of the following components: glucose 1 g / L, ammonium chloride 0.2 g / L, potassium dihydrogen phosphate 0.05 g / L, magnesium sulfate heptahydrate 0.01 g / L, calcium chloride 0.01 g / L, ferrous sulfate heptahydrate 0.01 g / L, yeast extract 0.1 g / L, sodium chloride 0 g / L, and agar 15 g / L.

[0055] Step 2: Inoculate the isolated single colony into liquid culture medium for amplification culture. The amplification culture conditions are: temperature 30℃, time 1 day, and rotation speed 160 rpm.

[0056] The liquid culture medium consists of the following components: glucose 1 g / L, ammonium chloride 0.2 g / L, potassium dihydrogen phosphate 0.05 g / L, magnesium sulfate heptahydrate 0.01 g / L, calcium chloride 0.01 g / L, ferrous sulfate heptahydrate 0.01 g / L, yeast extract 0.1 g / L, and sodium chloride 0 g / L.

[0057] Step 3: Centrifuge the amplified bacterial culture at 4000 rpm to remove the supernatant and obtain bacterial cells. Mix 0.01 g / L of bacterial cells with 3 g / L of activated sludge (SVI of 200 mL / g) and inoculate into a sequencing batch reactor. Settling time is 30 minutes, hydraulic retention time (HRT) is 12 hours, and water exchange ratio is 50%.

[0058] The reactor influent was simulated wastewater, with the following specific components: sodium chloride 30 g / L, glucose 0.750 g / L, sodium acetate 0.256 g / L, ammonium chloride 0.191 g / L, potassium dihydrogen phosphate 0.044 g / L, magnesium sulfate heptahydrate 0.037 g / L, calcium chloride 0.04 g / L, ferrous sulfate heptahydrate 0.037 g / L, and trace element solution 0.1 mL / L.

[0059] The trace element solution contains the following components: boric acid 0.5 g / L, copper chloride 0.3 g / L, manganese chloride tetrahydrate 1 g / L, ammonium heptamolybdate tetrahydrate 0.5 g / L, zinc chloride 0.5 g / L, cobalt chloride hexahydrate 0.5 g / L, aluminum chloride 0.5 g / L, and nickel chloride 0.5 g / L.

[0060] At the start of reactor operation, a significant amount of sludge was discharged. After 3 days, the sludge concentration decreased to 2.5 g / L. After 7 days of continuous operation, sludge settling properties improved significantly, with the SVI decreasing to 100 mL / g, and a small number of sludge aggregates were visible to the naked eye. After 14 days of continuous operation, the SVI decreased to 80 mL / g, the number of sludge aggregates increased, the average sludge particle size was 150 μm, and the sludge concentration recovered to 3.7 g / L. The removal rates of TOC, ammonia nitrogen, and total nitrogen in the wastewater were 95%, 82%, and 80%, respectively. Compared to the control group, the SVI decreased significantly (80 mL / g vs 150 mL / g), indicating that the addition of flocculants can effectively improve the settling performance of activated sludge.

[0061] Example 2: Cultivation of alkalophilic halophilic bacillus NCSL-JXB2 and the use of an enhanced attached biofilm process for treating pickled mustard tuber wastewater.

[0062] This embodiment includes the following steps:

[0063] Step 1: Take the bacterial culture from the preservation tube containing this strain and streak it on a solid culture medium for isolation and activation culture. The activation culture conditions are 30℃ and 6 days.

[0064] The solid culture medium consists of the following components: glucose 100 g / L, ammonium chloride 20 g / L, potassium dihydrogen phosphate 8 g / L, magnesium sulfate heptahydrate 5 g / L, calcium chloride 5 g / L, ferrous sulfate heptahydrate 5 g / L, yeast extract 5 g / L, sodium chloride 120 g / L, and agar 22 g / L.

[0065] Step 2: Inoculate the isolated single colony into liquid culture medium for amplification culture. The amplification culture conditions are 30℃, 4 days, and 160 rpm.

[0066] The liquid culture medium consists of the following components: glucose 100 g / L, ammonium chloride 20 g / L, potassium dihydrogen phosphate 8 g / L, magnesium sulfate heptahydrate 5 g / L, calcium chloride 5 g / L, ferrous sulfate heptahydrate 5 g / L, yeast extract 5 g / L, and sodium chloride 120 g / L.

[0067] Step 3: Centrifuge the amplified bacterial culture at 4000 rpm to remove the supernatant and obtain the bacterial cells. Mix the bacterial cells with 5 g / L of flocculants and 1 g / L of activated sludge, and inoculate them into a biofilm reactor containing fiber and sponge packing. The HRT is 36 hours, the settling time is 30 minutes, and the water exchange ratio is 50%.

[0068] The reactor influent is pickled mustard tuber wastewater, with the following specific parameters: TDS 120g / L, chemical oxygen demand (COD) 2300mg / L, ammonia nitrogen 340mg / L, and total phosphorus 53mg / L.

[0069] After 7 days of continuous operation, the reactor packing material was largely covered with biofilm. After 12 days of continuous operation, biofilm formation was complete, 7 days earlier than the control group which was only inoculated with activated sludge. The biofilm was deep yellow, dense, and difficult to peel off, with a thickness of 300 μm, while the control group's biofilm was only 200 μm thick and easily peeled off. The final removal rates for COD, ammonia nitrogen, total nitrogen, and total phosphorus in the wastewater were 93%, 80%, 75%, and 90%, respectively.

[0070] Example 3: Cultivation of alkalophilic halophilic bacillus NCSL-JXB2 and the use of an enhanced attached biofilm process for treating soy sauce brewing wastewater.

[0071] This embodiment includes the following steps:

[0072] Step 1: Take the bacterial culture from the preservation tube containing this strain and streak it on a solid culture medium for isolation and activation culture. The activation culture conditions are 30℃ and 3 days.

[0073] The solid culture medium consists of the following components: 20 g / L sucrose, 4 g / L ammonium chloride, 2 g / L potassium dihydrogen phosphate, 1 g / L magnesium sulfate heptahydrate, 1 g / L calcium chloride, 1 g / L ferrous sulfate heptahydrate, 1 g / L yeast extract, 60 g / L sodium chloride, and 20 g / L agar.

[0074] Step 2: Inoculate the isolated single colony into liquid culture medium for amplification culture. The amplification culture conditions are: temperature 30℃, time 2 days, and rotation speed 160 rpm.

[0075] The liquid culture medium consists of the following components: 20 g / L sucrose, 4 g / L ammonium chloride, 2 g / L potassium dihydrogen phosphate, 1 g / L magnesium sulfate heptahydrate, 1 g / L calcium chloride, 1 g / L ferrous sulfate heptahydrate, 1 g / L yeast extract, and 60 g / L sodium chloride.

[0076] Step 3: Centrifuge the amplified bacterial culture at 4000 rpm to remove the supernatant and obtain bacterial cells. Mix the bacterial cells with 1 g / L of flocculants and 3 g / L of activated sludge, and inoculate them into a biofilm reactor containing fiber and sponge packing. The HRT is 12 hours, the settling time is 30 minutes, and the water exchange ratio is 50%.

[0077] The reactor influent is soy sauce brewing wastewater, with the following specific parameters: TDS 65g / L, COD 1000mg / L, ammonia nitrogen 170mg / L, and total nitrogen 205mg / L.

[0078] After 6 days of continuous operation, the reactor packing material was largely covered with biofilm. After 11 days of continuous operation, biofilm formation was complete, 6 days earlier than the control group which only inoculated with activated sludge. The biofilm was deep yellow, dense, and difficult to peel off, with a thickness of 250 μm, compared to the control group's biofilm thickness of only 170 μm, which was easily peeled off. The final removal rates for COD, ammonia nitrogen, and total nitrogen in the wastewater were 95%, 90%, and 88%, respectively.

[0079] Example 4: Cultivation of alkalophilic halophilic bacillus NCSL-JXB2 and its assisted enhanced attached biofilm process for treating fish pickling wastewater

[0080] This embodiment includes the following steps:

[0081] Step 1: Take the bacterial culture from the preservation tube containing this strain and streak it on a solid culture medium for isolation and activation culture. The activation culture conditions are 30℃ and 3 days.

[0082] The solid culture medium consists of the following components: starch 20 g / L, ammonium chloride 4 g / L, potassium dihydrogen phosphate 2 g / L, magnesium sulfate heptahydrate 1 g / L, calcium chloride 1 g / L, ferrous sulfate heptahydrate 1 g / L, yeast extract 1 g / L, sodium chloride 100 g / L, and agar 22 g / L.

[0083] Step 2: Inoculate the isolated single colony into liquid culture medium for amplification culture. The amplification culture conditions are: temperature 30℃, time 2 days, and rotation speed 160 rpm.

[0084] The liquid culture medium consists of the following components: starch 20 g / L, ammonium chloride 4 g / L, potassium dihydrogen phosphate 2 g / L, magnesium sulfate heptahydrate 1 g / L, calcium chloride 1 g / L, ferrous sulfate heptahydrate 1 g / L, yeast extract 1 g / L, and sodium chloride 100 g / L.

[0085] Step 3: Centrifuge the amplified bacterial culture at 4000 rpm to remove the supernatant and obtain cell bodies. Mix the cells with flocculants at a ratio of 0.5 g / L and activated sludge at 2.5 g / L, and inoculate the mixture into a biofilm reactor containing fiber and sponge packing. The HRT is 24 hours, the settling time is 30 minutes, and the water exchange ratio is 50%.

[0086] The reactor influent is fish pickling wastewater, with the following specific parameters: TDS 100g / L, COD 2500mg / L, ammonia nitrogen 400mg / L, and total phosphorus 45mg / L.

[0087] After 11 days of continuous operation, biofilm formation was completed, 9 days earlier than the control group inoculated only with activated sludge. The biofilm was dark brown, dense, and difficult to peel off, with a thickness of 260 μm, while the control group's biofilm was only 180 μm thick and easily peeled off. The final removal rates for COD, ammonia nitrogen, total nitrogen, and total phosphorus in the wastewater were 95%, 84%, 80%, and 88%, respectively.

[0088] Example 5: Cultivation of Alkalophilic Halomonas NCSL-JXB2 and Assisted Enhancement of Attached Biofilm Process for Treating Aquaculture Wastewater

[0089] This embodiment includes the following steps:

[0090] Step 1: Take the bacterial culture from the preservation tube containing this strain and streak it on a solid culture medium for isolation and activation culture. The activation culture conditions are 30℃ and 2 days.

[0091] The solid culture medium consists of the following components: sodium acetate 20 g / L, ammonium chloride 4 g / L, potassium dihydrogen phosphate 2 g / L, magnesium sulfate heptahydrate 1 g / L, calcium chloride 1 g / L, ferrous sulfate heptahydrate 1 g / L, yeast extract 1 g / L, sodium chloride 30 g / L, and agar 18 g / L.

[0092] Step 2: Inoculate the isolated single colony into liquid culture medium for amplification culture. The amplification culture conditions are: temperature 30℃, time 1 day, and rotation speed 160 rpm.

[0093] The liquid culture medium consists of the following components: sodium acetate 20 g / L, ammonium chloride 4 g / L, potassium dihydrogen phosphate 2 g / L, magnesium sulfate heptahydrate 1 g / L, calcium chloride 1 g / L, ferrous sulfate heptahydrate 1 g / L, yeast extract 1 g / L, and sodium chloride 30 g / L.

[0094] Step 3: Centrifuge the amplified bacterial culture at 4000 rpm to remove the supernatant and obtain cell bodies. Mix the cells with flocculants at a ratio of 0.5 g / L and activated sludge at 2.5 g / L, and inoculate the mixture into a biofilm reactor containing fiber and sponge packing. The HRT is 8 hours, the settling time is 30 minutes, and the water exchange ratio is 50%.

[0095] The reactor influent is marine aquaculture wastewater, with the following specific parameters: TDS 40g / L, COD 300mg / L, ammonia nitrogen 80mg / L, and total phosphorus 18mg / L.

[0096] After 14 days of continuous operation, the reactor packing material was largely covered with biofilm. After 25 days of continuous operation, biofilm formation was complete; compared to the control group inoculated only with activated sludge, biofilm formation was completed 11 days earlier. The biofilm was dark brown, dense, and difficult to peel off, with a thickness of 270 μm. The final removal rates of COD, ammonia nitrogen, total nitrogen, and total phosphorus in the wastewater were 89%, 95%, 87%, and 74%, respectively.

[0097] Example 6: Cultivation of alkalophilic halophilic bacillus NCSL-JXB2 and its role in enhancing the granular treatment of oil and natural gas wastewater with activated sludge.

[0098] This embodiment includes the following steps:

[0099] Step 1: Take the bacterial culture from the preservation tube containing this strain and streak it on a solid culture medium for isolation and activation culture. The activation culture conditions are 30℃ and 6 days.

[0100] The solid culture medium consists of the following components: molasses 20 g / L, ammonium chloride 4 g / L, potassium dihydrogen phosphate 2 g / L, magnesium sulfate heptahydrate 1 g / L, calcium chloride 1 g / L, ferrous sulfate heptahydrate 1 g / L, yeast extract 1 g / L, sodium chloride 250 g / L, and agar 25 g / L.

[0101] Step 2: Inoculate the isolated single colony into liquid culture medium for amplification culture. The amplification culture conditions are 30℃, 4 days, and 160 rpm.

[0102] The liquid culture medium consists of the following components: molasses 20 g / L, ammonium chloride 4 g / L, potassium dihydrogen phosphate 2 g / L, magnesium sulfate heptahydrate 1 g / L, calcium chloride 1 g / L, ferrous sulfate heptahydrate 1 g / L, yeast extract 1 g / L, and sodium chloride 250 g / L.

[0103] Step 3: Centrifuge the amplified bacterial culture at 6000 rpm to remove the supernatant and obtain the bacterial cells. Inoculate 1 g / L of flocculants and 3 g / L of activated sludge (SVI of 200 mL / g, average particle size of 100 μm) into the SBR reactor. The reactor height-to-diameter ratio is 10, the HRT is 24 hours, the water exchange ratio is 50%, and the settling time is 3-15 minutes, adjusted according to specific conditions. The gas velocity is 8 L / min, the dissolved oxygen is 6 mg / L, and the temperature is room temperature.

[0104] The reactor influent is oil and natural gas wastewater, with the following specific parameters: TDS 250 g / L, COD 1800 mg / L, and ammonia nitrogen 120 mg / L.

[0105] After 10 days of continuous reactor operation, most of the activated sludge was discharged, and the sludge concentration decreased to 1 g / L, with small particles appearing. After 25 days of continuous operation, the proportion of small particles increased, the sludge particle size increased to 150 μm, the SVI was 90 mL / g, and the sludge concentration did not change significantly, remaining between 1.8 and 2.3 g / L. After 45 days of continuous operation, the sludge particle size increased to 210 μm, the particles were more irregular, the SVI was 50 mL / g, and complete granulation was achieved. The final removal rates of TOC, ammonia nitrogen, and total nitrogen in the wastewater were 89%, 50%, and 48%, respectively.

[0106] Example 7: Cultivation of alkalophilic halometabolite NCSL-JXB2 and its interaction with phenol-degrading bacteria to form phenol-degrading granular sludge for treating chemical industry wastewater.

[0107] This embodiment includes the following steps:

[0108] Step 1: Take the bacterial culture from the preservation tube containing this strain and streak it on a solid culture medium for isolation and activation culture. The activation culture conditions are 30℃ and 2 days.

[0109] The solid culture medium has the following components: glycerol 30 g / L, ammonium chloride 4 g / L, potassium dihydrogen phosphate 2 g / L, magnesium sulfate heptahydrate 1 g / L, calcium chloride 1 g / L, ferrous sulfate heptahydrate 1 g / L, yeast extract 1 g / L, sodium chloride 35 g / L, and agar 18 g / L.

[0110] Step 2: Inoculate the isolated single colony into liquid culture medium for amplification culture. The amplification culture conditions are 30℃, 1 day, and 150 rpm.

[0111] The liquid culture medium consists of the following components: glycerol 30 g / L, ammonium chloride 4 g / L, potassium dihydrogen phosphate 2 g / L, magnesium sulfate heptahydrate 1 g / L, calcium chloride 1 g / L, ferrous sulfate heptahydrate 1 g / L, yeast extract 1 g / L, and sodium chloride 35 g / L.

[0112] Step 3: Centrifuge the amplified bacterial culture at 4000 rpm to remove the supernatant and obtain the bacterial cells. Inoculate 1 g / L of flocculants and 1 g / L of chlorinated aromatic hydrocarbon-tolerant phenol-degrading bacteria (Cupriavidus necator) into the SBR reactor. The reactor height-to-diameter ratio is 10, the HRT is 24 hours, the water exchange ratio is 50%, and the settling time is 3-20 minutes, adjusted according to specific conditions. The gas velocity is 8 L / min, the dissolved oxygen is 6 mg / L, and the temperature is room temperature.

[0113] The reactor influent is wastewater from a chemical industry, with the following specific parameters: TDS 50g / L, COD 1800mg / L, 4-chlorophenol 150mg / L, and acute toxicity (luminescent bacteria inhibition rate) 85%.

[0114] After 12 days of continuous reactor operation, the sludge concentration decreased to 1 g / L, and noticeable small yellow particles appeared, with a particle size increasing to 50 μm. After 30 days of continuous operation, the proportion of small yellow particles increased, the average sludge particle size increased to 140 μm, and the SVI was 85 mL / g, while the sludge concentration did not change significantly. After 50 days of continuous operation, the sludge particle size increased to 350 μm, and the SVI was 55 mL / g, achieving complete granulation and forming rounded, compact, yellow phenol-degrading granular sludge. The control group without flocculant addition did not form aerobic granular sludge. The final wastewater COD removal rate was 97%, the effluent total nitrogen and ammonia nitrogen concentrations were less than 5 mg / L, the effluent 4-chlorophenol concentration was less than 5 mg / L, and the acute toxicity was less than 10%.

[0115] Example 8: Cultivation of alkalophilic halometa NCSL-JXB2 and its interaction with pyridine-degrading bacteria to form pyridine-degrading granular sludge for treating pharmaceutical industry wastewater.

[0116] This embodiment includes the following steps:

[0117] Step 1: Take the bacterial culture from the preservation tube containing this strain and streak it on a solid culture medium for isolation and activation culture. The activation culture conditions are 30℃ and 2 days.

[0118] The solid culture medium consists of the following components: glucose 20 g / L, ammonium chloride 2 g / L, potassium dihydrogen phosphate 1 g / L, magnesium sulfate heptahydrate 0.5 g / L, calcium chloride 0.25 g / L, ferrous sulfate heptahydrate 0.25 g / L, yeast extract 1 g / L, sodium chloride 30 g / L, and agar 18 g / L.

[0119] Step 2: Inoculate the isolated single colony into liquid culture medium for amplification culture. The amplification culture conditions are: temperature 30℃, time 1 day, and rotation speed 160 rpm.

[0120] The liquid culture medium consists of the following components: glucose 20 g / L, ammonium chloride 2 g / L, potassium dihydrogen phosphate 1 g / L, magnesium sulfate heptahydrate 0.5 g / L, calcium chloride 0.25 g / L, ferrous sulfate heptahydrate 0.25 g / L, yeast extract 1 g / L, and sodium chloride 30 g / L.

[0121] Step 3: Centrifuge the amplified bacterial culture at 4000 rpm to remove the supernatant and obtain the bacterial cells. Inoculate 1 g / L of flocculants and 2 g / L of pyridine-degrading bacteria into the SBR reactor. The reactor height-to-diameter ratio is 10, the HRT is 24 hours, the water exchange ratio is 50%, and the settling time is 3-30 minutes, which can be adjusted according to the specific conditions. The gas velocity is 8 L / min, the dissolved oxygen is 6 mg / L, and the temperature is room temperature.

[0122] The reactor influent is pharmaceutical wastewater with the following specific parameters: TDS 30g / L, COD 1500mg / L, and pyridine content 100mg / L.

[0123] After 13 days of continuous reactor operation, the sludge concentration decreased to 1.3 g / L, with noticeable small yellow particles appearing and the particle size increasing to 50 μm. After 28 days of continuous operation, the proportion of brownish-yellow particles increased, the average sludge particle size increased to 160 μm, and the SVI was 98 mL / g, while the sludge concentration did not change significantly. After 40 days of continuous operation, the sludge particle size increased to 250 μm, and the SVI was 45 mL / g, achieving complete granulation and forming rounded, compact, brownish-yellow pyridine-degrading granular sludge. The control group without flocculant addition did not form aerobic granular sludge. The final wastewater COD removal rate was 85%, the pyridine concentration was less than 7 mg / L, and the total nitrogen and ammonia nitrogen concentrations were less than 10 mg / L.

[0124] Example 9: Cultivation of alkalophilic halometa NCSL-JXB2 and its synergistic effect with Penicillium fimbriae mycelial balls to rapidly form ultra-high salt-tolerant granular sludge.

[0125] This embodiment includes the following steps:

[0126] Step 1: Take the bacterial culture from the preservation tube containing this strain and streak it on a solid culture medium for isolation and activation culture. The activation culture conditions are 30℃ and 6 days.

[0127] The solid culture medium consists of the following components: glucose 20 g / L, ammonium chloride 2 g / L, potassium dihydrogen phosphate 1 g / L, magnesium sulfate heptahydrate 0.5 g / L, calcium chloride 0.25 g / L, ferrous sulfate heptahydrate 0.25 g / L, yeast extract 1 g / L, sodium chloride 120 g / L, and agar 22 g / L.

[0128] Step 2: Inoculate the isolated single colony into liquid culture medium for amplification culture. The amplification culture conditions are 30℃, 4 days, and 160 rpm.

[0129] The liquid culture medium consists of the following components: glucose 20 g / L, ammonium chloride 2 g / L, potassium dihydrogen phosphate 1 g / L, magnesium sulfate heptahydrate 0.5 g / L, calcium chloride 0.25 g / L, ferrous sulfate heptahydrate 0.25 g / L, yeast extract 1 g / L, and sodium chloride 120 g / L.

[0130] Step 3: Centrifuge the amplified bacterial culture at 4000 rpm to remove the supernatant and obtain the bacterial cells. Inoculate 1 g / L of flocculent bacteria and 3 g / L of Penicillium fibrillosa mycelial balls into the SBR reactor. The reactor height-to-diameter ratio is 10, the HRT is 24 hours, the water exchange ratio is 50%, and the reactor is operated in an anaerobic-aerobic mode. The settling time is 3-10 minutes, which can be adjusted according to the specific conditions. The gas velocity is 8 L / min, the dissolved oxygen is 6 mg / L, and the temperature is room temperature.

[0131] The reactor influent is pickled mustard tuber wastewater, with the following specific parameters: TDS 120g / L, COD 2300mg / L, ammonia nitrogen 340mg / L, and total phosphorus 53mg / L.

[0132] After 14 days of continuous reactor operation, the particle size increased to 570 μm. The surface of the mycelial balls showed a yellow color, with a high concentration of flocculants and insoluble particles from the wastewater adhering to the surface. The SVI (solution volume index) was 48 mL / g, meeting the granulation standard, indicating that the mycelial balls had successfully transformed into granular sludge. After 30 days of continuous operation, the average sludge particle size increased to 760 μm, the SVI was 38 mL / g, and the sludge concentration increased to 7.3 g / L. The final removal rates for COD, ammonia nitrogen, total nitrogen, and total phosphorus in the wastewater were 95%, 85%, 82%, and 95%, respectively.

[0133] Example 10: Cultivation of alkalophilic halometa NCSL-JXB2 and its interaction with nitrifying bacteria to form highly efficient simultaneous nitrification and denitrification (SND) granular sludge.

[0134] This embodiment includes the following steps:

[0135] Step 1: Take the bacterial culture from the preservation tube containing this strain and streak it on a solid culture medium for isolation and activation culture. The activation culture conditions are 30℃ and 3 days.

[0136] The solid culture medium consists of the following components: 20 g / L sucrose, 4 g / L ammonium chloride, 2 g / L potassium dihydrogen phosphate, 1 g / L magnesium sulfate heptahydrate, 1 g / L calcium chloride, 1 g / L ferrous sulfate heptahydrate, 1 g / L yeast extract, 60 g / L sodium chloride, and 20 g / L agar.

[0137] Step 2: Inoculate the isolated single colony into liquid culture medium for amplification culture. The amplification culture conditions are: temperature 30℃, time 2 days, and rotation speed 160 rpm.

[0138] The liquid culture medium consists of the following components: 20 g / L sucrose, 4 g / L ammonium chloride, 2 g / L potassium dihydrogen phosphate, 1 g / L magnesium sulfate heptahydrate, 1 g / L calcium chloride, 1 g / L ferrous sulfate heptahydrate, 1 g / L yeast extract, and 60 g / L sodium chloride.

[0139] Step 3: Centrifuge the amplified bacterial culture at 4000 rpm to remove the supernatant and obtain the bacterial cells. Inoculate 1 g / L of flocculants and 3 g / L of nitrifying bacteria into the SBR reactor. The reactor height-to-diameter ratio is 10, the HRT is 18 hours, the water exchange ratio is 50%, and the settling time is 3-30 minutes, which can be adjusted according to the specific conditions. The gas velocity is 8 L / min, the dissolved oxygen is 6 mg / L, and the temperature is room temperature.

[0140] The reactor influent is soy sauce brewing wastewater, with the following specific parameters: TDS 65g / L, COD 1000mg / L, ammonia nitrogen 170mg / L, and total nitrogen 205mg / L.

[0141] After 15 days of continuous reactor operation, the sludge concentration decreased to 1.8 g / L, with noticeable small brown particles appearing and the particle size increasing to 48 μm. After 30 days of continuous operation, the proportion of small brown particles increased, the average sludge particle size increased to 113 μm, and the SVI was 80 mL / g, while the sludge concentration did not change significantly. After 60 days of continuous operation, the sludge particle size increased to 640 μm, and the SVI was 35 mL / g, achieving complete granulation and forming rounded, compact, brown, high-efficiency SND granular sludge. The control group without flocculant addition did not form aerobic granular sludge. The final removal rates of COD, total nitrogen, and ammonia nitrogen in the wastewater were 97%, 95%, and 97%, respectively.

[0142] Example 11: Cultivation of alkalophilic halometa NCSL-JXB2 and its use as a standalone additive in a enhanced biological contact oxidation process for treating textile dyeing wastewater.

[0143] This embodiment includes the following steps:

[0144] Step 1: Take the bacterial culture from the preservation tube containing this strain and streak it on a solid culture medium for isolation and activation culture. The activation culture conditions are 30℃ and 3 days.

[0145] The solid culture medium consists of the following components: glucose 20 g / L, ammonium chloride 4 g / L, potassium dihydrogen phosphate 2 g / L, magnesium sulfate heptahydrate 1 g / L, calcium chloride 1 g / L, ferrous sulfate heptahydrate 1 g / L, yeast extract 1 g / L, sodium chloride 60 g / L, and agar 20 g / L.

[0146] Step 2: Inoculate the isolated single colony into liquid culture medium for amplification culture. The amplification culture conditions are: temperature 30℃, time 2 days, and rotation speed 160 rpm.

[0147] The liquid culture medium consists of the following components: glucose 20 g / L, ammonium chloride 4 g / L, potassium dihydrogen phosphate 2 g / L, magnesium sulfate heptahydrate 1 g / L, calcium chloride 1 g / L, ferrous sulfate heptahydrate 1 g / L, yeast extract 1 g / L, and sodium chloride 60 g / L.

[0148] Step 3: Centrifuge the amplified bacterial culture at 4000 rpm to remove the supernatant and obtain the bacterial cells. Mix the bacterial cells with 1 g / L of flocculants and 3 g / L of activated sludge from the system, and inoculate them into a biofilm reactor containing fiber and sponge packing. The HRT is 48 hours, the settling time is 30 minutes, and the water exchange ratio is 50%.

[0149] The reactor influent is textile dyeing and printing wastewater, with the following specific parameters: TDS 60g / L, COD 2300mg / L, and total nitrogen 55mg / L.

[0150] After 10 days of continuous operation, the reactor packing material was largely covered with biofilm. After 32 days of continuous operation, biofilm formation was complete, 14 days earlier than the control group which only inoculated with activated sludge. The biofilm was brownish-red, dense, and difficult to peel off, with a thickness of 260 μm, compared to only 100 μm in the control group. The wastewater COD and total nitrogen removal rates were 65% and 80%, respectively.

[0151] Example 12: Cultivation of Alkalophilic Halomonas NCSL-JXB2 and its treatment of reverse osmosis concentrate by single addition

[0152] This embodiment includes the following steps:

[0153] Step 1: Take the bacterial culture from the preservation tube containing this strain and streak it on a solid culture medium for isolation and activation culture. The activation culture conditions are 30℃ and 3 days.

[0154] The solid culture medium consists of the following components: glucose 20 g / L, ammonium chloride 4 g / L, potassium dihydrogen phosphate 2 g / L, magnesium sulfate heptahydrate 1 g / L, calcium chloride 1 g / L, ferrous sulfate heptahydrate 1 g / L, yeast extract 1 g / L, sodium chloride 60 g / L, and agar 20 g / L.

[0155] Step 2: Inoculate the isolated single colony into liquid culture medium for amplification culture. The amplification culture conditions are: temperature 30℃, time 2 days, and rotation speed 160 rpm.

[0156] The liquid culture medium consists of the following components: glucose 20 g / L, ammonium chloride 4 g / L, potassium dihydrogen phosphate 2 g / L, magnesium sulfate heptahydrate 1 g / L, calcium chloride 1 g / L, ferrous sulfate heptahydrate 1 g / L, yeast extract 1 g / L, and sodium chloride 60 g / L.

[0157] Step 3: Centrifuge the amplified bacterial culture at 4000 rpm to remove the supernatant and obtain bacterial cells. Inoculate the cells into a shake flask at a ratio of 3 g / L. The HRT is 24 hours, the settling time is 30 minutes, the water exchange ratio is 50%, the temperature is 30℃, and the rotation speed is 160 rpm.

[0158] The reactor feedwater is reverse osmosis concentrate, with the following specific parameters: TDS (mainly sodium sulfate, accounting for 86% of the total TDS) 65 g / L, COD 400 mg / L, total nitrogen 25 mg / L, and ammonia nitrogen 15 mg / L.

[0159] After 3 days of continuous operation, there were no significant changes in biomass and particle size in the shake flasks, and the removal rates of COD, ammonia nitrogen, and total nitrogen in the wastewater were 36%, 64%, and 60%, respectively. After 15 days of continuous operation, the biomass in the shake flasks increased to 5 g / L, and small particles appeared, with an average particle size increase of 100 μm. The removal rates of COD, ammonia nitrogen, and total nitrogen in the wastewater were 47%, 75%, and 72%, respectively. After glucose supplementation for induction, the removal rates of COD, ammonia nitrogen, and total nitrogen in the wastewater could be increased to 75%, 89%, and 86%, respectively.

[0160] Example 13: Cultivation of alkalophilic halophilic bacillus NCSL-JXB2 and its use as a standalone additive in a enhanced biological contact oxidation process for treating electrodialysis concentrate.

[0161] This embodiment includes the following steps:

[0162] Step 1: Take the bacterial culture from the preservation tube containing this strain and streak it on a solid culture medium for isolation and activation culture. The activation culture conditions are 30℃ and 2 days.

[0163] The solid culture medium consists of the following components: glucose 20 g / L, ammonium chloride 4 g / L, potassium dihydrogen phosphate 2 g / L, magnesium sulfate heptahydrate 1 g / L, calcium chloride 1 g / L, ferrous sulfate heptahydrate 1 g / L, yeast extract 1 g / L, sodium chloride 180 g / L, and agar 23 g / L.

[0164] Step 2: Inoculate the isolated single colony into liquid culture medium for amplification culture. The amplification culture conditions are: temperature 30℃, time 1 day, and rotation speed 160 rpm.

[0165] The liquid culture medium consists of the following components: glucose 20 g / L, ammonium chloride 4 g / L, potassium dihydrogen phosphate 2 g / L, magnesium sulfate heptahydrate 1 g / L, calcium chloride 1 g / L, ferrous sulfate heptahydrate 1 g / L, yeast extract 1 g / L, and sodium chloride 180 g / L.

[0166] Step 3: Centrifuge the amplified bacterial culture at 4000 rpm to remove the supernatant and obtain the bacterial cells. Mix the flocculants at a ratio of 1 g / L to 3 g / L of activated sludge from the system and inoculate them into a biofilm reactor containing fiber and sponge packing. The HRT is 24 hours, the settling time is 30 minutes, and the water exchange ratio is 50%.

[0167] The reactor feedwater is electrodialysis concentrate with the following specific parameters: TDS 180 g / L, conductivity 260 mS / cm, COD 200 mg / L, and ammonia nitrogen 20 mg / L.

[0168] After 15 days of continuous operation, the reactor packing material was mostly covered with biofilm. After 37 days of continuous operation, biofilm formation was complete, while the control group failed to start up successfully. The biofilm was yellowish-brown, dense, and difficult to peel off, with a thickness of 160 μm. The final removal rates of COD, ammonia nitrogen, and total nitrogen in the wastewater were 45%, 53%, and 47%, respectively.

[0169] Based on the results of Examples 1-13 above, it can be seen that the *Halomonas alkaliphila* NCSL-JXB2 strain of the present invention has a wide salt tolerance range, can survive in salinity environments of 0-25 wt%, and exhibits good flocculation effects with a self-flocculation rate of 66.3%. This strain can also be used as a bioaugmentation aid to enhance other biological processes, such as combining it with activated sludge processes to improve sludge settling performance; combining it with biofilm processes to enhance film formation speed and prevent film detachment; combining it with activated sludge or mycelial balls to rapidly form aerobic granular sludge; and co-adding it with nitrifying bacteria or toxic organic matter degrading bacteria to rapidly form high-efficiency SND granular sludge, specific toxic and recalcitrant organic matter degrading granular sludge (such as pyridine, phenol, etc.), and other functional aerobic granular sludge. This strain can also be inoculated alone into high-salt organic wastewater to remove organic matter and simultaneously flocculate and remove suspended solids.

[0170] The above description is merely a preferred embodiment for explaining the present invention and is not intended to limit the present invention in any way. Therefore, any modifications or changes made to the present invention under the same inventive spirit should still be included within the scope of protection intended by the present invention.

Claims

1. A strain of flocculating bacterium *Halomonas alkaliphila* NCSL-JXB2, characterized in that... Its accession number is CGMCC No.33827.

2. The alkalophilic halophilic bacterium (Halomonas alkaliphila) NCSL-JXB2 according to claim 1, characterized in that, Its 16S rDNA nucleotide sequence is shown in SEQ ID NO.

1.

3. The method for culturing Halomonas alkaliphila NCSL-JXB2 according to claim 1 or 2, characterized in that, Includes the following steps: Step 1: Inoculate the strain into a solid culture medium and activate it at 30°C for 2–6 days; Step 2: Transfer single colonies to liquid culture medium and amplify them at 30°C for 1–4 days; The liquid culture medium consists of: carbon source 1–100 g / L, ammonium chloride 0.2–20 g / L, potassium dihydrogen phosphate 0.05–8 g / L, magnesium sulfate heptahydrate 0.01–5 g / L, calcium chloride 0.01–5 g / L, ferrous sulfate heptahydrate 0.01–5 g / L, yeast extract 0.1–5 g / L, and sodium chloride 0–250 g / L. The carbon source is selected from one or more combinations of glucose, sucrose, starch, sodium acetate, glycerol, and molasses; The solid culture medium in step 1 consists of the liquid culture medium from step 2 plus 15-25 g / L agar.

4. The application of Halomonas alkaliphila NCSL-JXB2 as described in claim 1 or 2, characterized in that, include: (1) As a co-addition of flocculants and activated sludge, it is used to assist and enhance the biological process for treating high-salt organic wastewater. (2) When added in combination with other functional strains, it is used to improve the operational efficiency of biological processes for treating high-salt organic wastewater; and (3) Add separately for the treatment of high-salt organic wastewater.

5. The application according to claim 4, characterized in that, The biological process for auxiliary and enhanced treatment of high-salt organic wastewater is selected from: The process can be enhanced by supplementing and strengthening suspended activated sludge processes, attached biofilm processes, and activated sludge granulation.

6. The application according to claim 4, characterized in that, The method of co-addition with other functional strains to improve the operational efficiency of biological processes for treating high-salt organic wastewater is selected from: It works in conjunction with phenol-degrading bacteria to form phenol-degrading granular sludge, with pyridine-degrading bacteria to form pyridine-degrading granular sludge, with Penicillium fimbriae mycelial balls to rapidly form ultra-high salt-tolerant granular sludge, and with nitrifying bacteria to form highly efficient synchronous nitrification and denitrification granular sludge.

7. The application according to claim 4, characterized in that, The high-salt organic wastewater is selected from one or more of the following: simulated wastewater, pickled mustard tuber wastewater, soy sauce brewing wastewater, fish pickling wastewater, seafood aquaculture wastewater, oil and gas wastewater, pharmaceutical wastewater, textile printing and dyeing wastewater, chemical wastewater, reverse osmosis concentrate, and electrodialysis concentrate.

8. The application according to claim 7, characterized in that, The main pollutants in the high-salt organic wastewater are organic matter, nitrogen, and phosphate.

9. The application according to claim 7, characterized in that, The salinity of the high-salt organic wastewater is expressed as sodium chloride, with a sodium chloride concentration range of 30–250 g / L; or the salinity of the high-salt organic wastewater is expressed as conductivity, with a conductivity range of 50–500 mS / cm; or the salinity of the high-salt organic wastewater is expressed as total dissolved solids, with a total dissolved solids content range of 30–330 g / L.

10. The application according to claim 4, characterized in that, This includes centrifuging the bacterial culture of Halomonas alkaliphila NCSL-JXB2 cultured according to the method of claim 3, collecting the bacterial cells, and adding them to the bioreactor at a bacterial cell concentration of 0.01–5 g / L to improve the operating effect of the bioprocess.

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