Feed additive for relieving animal mycotoxin poisoning and preparation method thereof

Abstract

The invention discloses a feed additive for relieving animal mycotoxin poisoning and a preparation method thereof. The feed additive comprises the following components in parts by weight: 25-30 parts of attapulgite, 25-30 parts of montmorillonite, 25-30 parts of yeast cell walls, 2-5 parts of vitamin E, 5-10 parts of taurine and 5-10 parts of lipoic acid. The feed additive disclosed by the invention can be used for remarkably improving the harm problem of mycotoxin in the livestock and poultry breeding production process and reducing the economic loss of livestock and poultry farmers caused by the mycotoxin pollution of the feed.

Description

Technical Field

[0001] The present invention relates to the technical field of livestock and poultry breeding and feed, and in particular to a feed additive for alleviating animal mycotoxin poisoning and a preparation method thereof. Background Art

[0002] Mycotoxins are toxic secondary metabolites produced by molds and are widely found in contaminated feed raw materials (such as corn, wheat, soybean meal, etc.) and finished feeds. According to the European Food Safety Authority (EFSA), the detection rate of mycotoxins in food crops worldwide is as high as 80%. @ A 2024 survey revealed that mycotoxin contamination in livestock and poultry feed is extremely serious in Asian countries. When animals ingest contaminated feed, they may suffer acute or chronic poisoning, leading to symptoms such as decreased productivity, weakened immunity, oxidative stress, organ damage, and, in severe cases, death. In recent years, mycotoxin contamination in feed has caused significant economic losses to the livestock and poultry industry. Therefore, finding feed additives that mitigate animal mycotoxicosis, reduce the harmful effects of mycotoxins on livestock and poultry, and mitigate the economic losses caused by mycotoxins is of great significance to the healthy development of the livestock and poultry industry.

[0003] Mycotoxins in feed are numerous, concealed, and contaminate a wide range of species, posing long-term and persistent risks to livestock and poultry health. Current mycotoxin adsorption technologies cannot effectively address the harmful effects of mycotoxins on livestock and poultry during production, and the market lacks feed additives that can effectively mitigate mycotoxin poisoning in animals.

[0004] The current mycotoxin prevention and control system primarily relies on adsorption technology, such as the selective adsorption of toxin molecules in feed using materials such as activated carbon, montmorillonite, and bentonite. However, this technology has many limitations, including differences in adsorption efficiency between different products (the adsorption rate of aflatoxin ranges from 35% to 90%), the effectiveness of most adsorption materials only against specific toxins (for example, montmorillonite has an adsorption rate of over 80% for aflatoxin B1, but less than 20% for vomitoxin), and an inability to address the complex contamination found in actual production (approximately 70% or more of two or more mycotoxins are detected in feed samples).

[0005] Based on this, breaking through the existing single prevention and control model and building a three-in-one composite prevention and control system of "preventing toxin absorption - enhancing detoxification - improving resistance" is of great significance for alleviating animal mycotoxin poisoning. Summary of the Invention

[0006] The present invention aims to overcome the shortcomings of the prior art and provides a feed additive for alleviating mycotoxin poisoning in animals and a method for preparing the same. The feed additive of the present invention can significantly alleviate the harmful effects of mycotoxins in livestock and poultry production, reducing the economic losses suffered by livestock and poultry farmers due to mycotoxin contamination in feed. The method for preparing the feed additive of the present invention can significantly alleviate symptoms such as decreased livestock and poultry production performance, serum biochemical indicators, antioxidant enzyme activity, mitochondrial structural abnormalities, and inflammation caused by mycotoxins.

[0007] To achieve the above purpose, the technical solution designed by the present invention is as follows:

[0008] The present invention provides a feed additive for alleviating mycotoxin poisoning in animals. The feed additive comprises, by weight, 25 to 30 parts of attapulgite, 25 to 30 parts of montmorillonite, 25 to 30 parts of yeast cell walls, 2 to 5 parts of vitamin E, 5 to 10 parts of taurine and 5 to 10 parts of lipoic acid.

[0009] Furthermore, the components of the feed additive include, by weight, 25 parts of attapulgite, 25 parts of montmorillonite, 25 parts of yeast cell walls, 5 parts of vitamin E, 10 parts of taurine and 10 parts of lipoic acid.

[0010] Furthermore, the effective mineral mass percentage of the attapulgite is ≥80%;

[0011] The effective mineral mass percentage of the montmorillonite is ≥90%;

[0012] The mass percentage of the effective components of the yeast cell wall is ≥78%, of which the mass percentage of β-glucan is ≥60% and the mass percentage of mannooligosaccharide is ≥18%;

[0013] The mass percentage of the active ingredient of vitamin E is ≥93%;

[0014] The effective ingredient mass percentage of taurine is ≥98%;

[0015] The effective component mass percentage of the lipoic acid is ≥98%.

[0016] The present invention also provides a method for preparing the feed additive, comprising the following steps:

[0017] (1) Weigh attapulgite, montmorillonite, yeast cell wall, vitamin E, taurine, and lipoic acid according to the above-mentioned parts by weight;

[0018] (2) mixing the weighed attapulgite, montmorillonite and yeast cell walls;

[0019] (3) Add the weighed vitamin E, taurine and lipoic acid and mix them to obtain a feed additive for alleviating animal mycotoxin poisoning.

[0020] The present invention also provides a use of the feed additive in preparing feed for alleviating animal mycotoxin poisoning.

[0021] The present invention also provides a feed for alleviating animal mycotoxin poisoning, wherein the feed comprises the feed additive.

[0022] Furthermore, the feed also includes a basic diet and feed additives, wherein the amount of the feed additives added is 0.1% to 0.2% of the total amount of the basic diet.

[0023] Furthermore, the feed additive is added in an amount of 0.2% of the total amount of the basic diet.

[0024] The present invention also provides a use of the feed additive or the feed in promoting the growth of meat ducks poisoned by mycotoxins.

[0025] The present invention also provides a use of the feed additive or the feed in alleviating poisoning symptoms of meat ducks poisoned by mycotoxins.

[0026] Principle of the present invention:

[0027] Due to their unique structures, attapulgite, montmorillonite, and yeast cell walls exhibit excellent adsorption properties for various toxins. Their combined use can produce a synergistic effect, enabling the co-adsorption of multiple toxins and preventing their absorption. Inducing oxidative stress and mitochondrial damage is a key pathological mechanism for the toxic effects of mycotoxins. Both vitamin E and taurine can enhance the body's antioxidant capacity, thereby alleviating the oxidative stress caused by mycotoxins. Lipoic acid not only has antioxidant properties but can also repair mitochondrial damage caused by mycotoxins. Combined use of these three ingredients can alleviate symptoms of mycotoxicosis. Furthermore, vitamin E, taurine, and lipoic acid possess anti-inflammatory and immune-enhancing properties. Therefore, the combined addition of attapulgite, montmorillonite, yeast cell walls, vitamin E, taurine, and lipoic acid to feed is an effective measure for alleviating mycotoxicosis in animals.

[0028] In response to the problems of the mycotoxin detoxification agents currently on the market, such as single function (mainly adsorbing toxins and having a narrow adsorption spectrum), large differences in adsorption efficiency, and insufficient ability to cope with complex pollution, the present invention combines the mechanism of action of mycotoxins and, on the basis of existing detoxification agents, further supplements functional active substances to improve the antioxidant capacity of livestock and poultry, alleviate mitochondrial damage, enhance the body's resistance and mycotoxin detoxification ability, thereby constructing a "preventing toxin absorption - enhancing detoxification - improving resistance" trinity composite prevention and control system, alleviating the harm of feed mycotoxin contamination to livestock and poultry, with clear control targets, definite effects, and economic feasibility. The implementation of the present invention can significantly alleviate the symptoms of mycotoxin poisoning in livestock and poultry, and reduce the economic losses caused by feed mycotoxin contamination to enterprises and farmers.

[0029] Beneficial effects of the present invention:

[0030] 1. Due to the wide range and variety of mycotoxins, as well as the poor effectiveness of commercially available detoxification / adsorbents, mycotoxin contamination in livestock and poultry production cannot be effectively controlled, resulting in significant economic losses. The product of this invention is effective in livestock farms and has a significant effect on improving symptoms of mycotoxin poisoning.

[0031] 2. The combined use of attapulgite, montmorillonite, and yeast cell walls in the present invention can adsorb various mycotoxins in feed, achieving greater adsorption than when used alone. The combined use of vitamin E, taurine, and lipoic acid in the present invention not only enhances the antioxidant capacity of animals and increases their ability to detoxify mycotoxins, but also repairs mitochondrial damage caused by mycotoxins and enhances their resistance, thereby jointly mitigating the harm of mycotoxins to livestock and poultry. The combined use of attapulgite, montmorillonite, yeast cell walls, vitamin E, taurine, and lipoic acid in the present invention demonstrates a significant effect in alleviating mycotoxin poisoning in animals.

[0032] 3. The feed additive of the present invention has a simple preparation method and low cost, which is conducive to industrial production. The feed additive of the present invention is added in an amount of 0.2%. Feed manufacturers do not need to pre-mix it during use and can directly add it to the main mixer. The application effect is obvious, which can significantly alleviate the symptoms of mycotoxin poisoning in animals and improve the production performance of animals. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 This is a diagram showing the effect of feed additives on improving the mitochondrial structure of meat duck liver. DETAILED DESCRIPTION

[0034] The present invention is further described in detail below with reference to specific embodiments so that those skilled in the art can understand.

[0035] Experimental Materials

[0036] 1. The attapulgite is feed grade, with effective mineral content ≥80%.

[0037] 2. Montmorillonite is feed grade, with effective mineral content ≥90%.

[0038] 3. The yeast cell wall is feed grade, and the mass percentage of active ingredients is ≥78% (including β-glucan mass percentage ≥60% and mannooligosaccharide mass percentage ≥18%).

[0039] 4. Vitamin E is feed grade, with the mass percentage of active ingredients ≥93%.

[0040] 5. Taurine is feed grade, with the effective ingredient mass percentage ≥98%.

[0041] 6. Lipoic acid is feed grade, with the effective ingredient mass percentage ≥98%.

[0042] Example 1

[0043] Feed additives to alleviate mycotoxin poisoning in animals

[0044] This embodiment provides a feed additive for alleviating mycotoxin poisoning in animals. The components of the feed additive include, by weight, 25 to 30 parts of attapulgite, 25 to 30 parts of montmorillonite, 25 to 30 parts of yeast cell walls, 2 to 5 parts of vitamin E, 5 to 10 parts of taurine, and 5 to 10 parts of lipoic acid.

[0045] Example 2

[0046] Feed additives to alleviate mycotoxin poisoning in animals

[0047] This embodiment provides a feed additive for alleviating mycotoxin poisoning in animals. The components of the feed additive include, by weight, 25 parts of attapulgite, 25 parts of montmorillonite, 25 parts of yeast cell walls, 5 parts of vitamin E, 10 parts of taurine, and 10 parts of lipoic acid.

[0048] Example 3

[0049] Preparation method of feed additive for alleviating animal mycotoxin poisoning

[0050] This embodiment provides a method for preparing the feed additive of embodiment 1 or embodiment 2, and the specific method is as follows:

[0051] 1. According to the weight parts of Example 1 or Example 2, attapulgite, montmorillonite, yeast cell wall, vitamin E, taurine and lipoic acid were weighed.

[0052] 2. Add the weighed attapulgite, montmorillonite and yeast cell wall into the mixer in sequence and mix for 3 to 5 minutes.

[0053] 3. Add the weighed vitamin E, taurine and lipoic acid into the mixer and continue mixing for 3 to 5 minutes to obtain a feed additive for alleviating animal mycotoxin poisoning.

[0054] Example 4

[0055] Feed to alleviate mycotoxicosis in animals

[0056] This embodiment provides a feed for alleviating mycotoxin poisoning in animals. The feed additive of Example 2 is added to the basic diet of meat ducks to obtain a feed for alleviating mycotoxin poisoning in animals. The amount of the feed additive added is 0.2% of the total amount of the basic diet.

[0057] Example 5

[0058] Application of feed to alleviate mycotoxin poisoning in animals

[0059] The meat ducks with mycotoxin poisoning were fed daily with the feed for alleviating animal mycotoxin poisoning of Example 4 as the additive group, the meat ducks with mycotoxin poisoning were fed daily with the basic diet of meat ducks as the mycotoxin group, and the healthy meat ducks were fed daily with the basic diet of meat ducks as the control group.

[0060] The additive group, mycotoxin group and control group were experimented under the same experimental conditions to test the growth performance, serum biochemical indicators, antioxidant enzyme activity and liver mitochondrial structure of the meat ducks. The growth performance, serum biochemical indicators, antioxidant enzyme activity and liver mitochondrial structure of the meat ducks in the additive group were better than those in the mycotoxin group.

[0061] Table 1 Effect of feed on the growth performance of meat ducks

[0062]

[0063]

[0064] Note: The results are expressed as mean ± standard deviation (n=6). Values ​​in the same row with different letters indicate significant differences (P<0.05).

[0065] As can be seen from Table 1, under the conditions of this experiment, the final weight and average daily weight gain of the meat ducks in the additive group in the first and second weeks were significantly increased by 11.21% and 14.81% compared with the mycotoxin group, and the feed-to-weight ratio was significantly decreased by 6.60%; the final weight and average daily feed intake of the meat ducks in the additive group in the third and fourth weeks were significantly increased by 10.61% and 14.48% compared with the mycotoxin group, and there was no significant difference in the average daily weight gain and feed-to-weight ratio.

[0066] Table 2 Effects of feed additives on improving serum biochemical indicators of meat ducks

[0067]

[0068]

[0069] Note: The results are expressed as mean ± standard deviation (n=6). Values ​​in the same row with different letters indicate significant differences (P<0.05).

[0070] As can be seen from Table 2, under the conditions of this experiment, compared with the mycotoxin group, the levels of AST (aspartate aminotransferase) and TBA (total bile acid) in the serum of the meat ducks in the additive group at the second weekend were significantly reduced by 33.47% and 35.10%, and the level of TP (serum total protein) was significantly increased by 43.77%; at the fourth weekend, the levels of AST, ALT (alanine aminotransferase), TBIL (total bilirubin) and TBA in the serum of the meat ducks in the additive group were significantly reduced by 24.27%, 19.87%, 31.25% and 23.90%, and the levels of TP and ALB (serum albumin) were significantly increased by 17.99% and 21.06%.

[0071] Table 3 Effect of feed additives on the improvement of antioxidant enzyme activity in duck liver

[0072]

[0073] Note: The results are expressed as mean ± standard deviation (n=6). Values ​​in the same row with different letters indicate significant differences (P<0.05).

[0074] As can be seen from Table 3, under the conditions of this experiment, compared with the mycotoxin group, at the end of the second week, the GPX (glutathione peroxidase) and SOD (superoxide dismutase) enzyme activities in the liver of the meat ducks in the additive group were significantly increased by 24.63% and 12.43%, the GSH (glutathione) enzyme activity was significantly increased by 25.32%, and the MDA (malondialdehyde) content was significantly decreased by 18.43%; at the end of the second week, the GPX enzyme activity in the liver of the meat ducks in the additive group was significantly increased by 42.79%, the GSH enzyme activity was significantly increased by 13.25%, and the MDA content was significantly decreased by 22.47%.

[0075] from Figure 1 As can be seen in the results, under these experimental conditions, the mycotoxin-treated hepatocytes underwent significant ultrastructural changes at the end of the fourth week. The nuclei showed irregular atrophy, numerous mitochondria were structurally disrupted and swollen, cristae were absent, and significant cytoplasmic vacuolation was observed. The additives restored the normal ultrastructural structure of hepatocytes to varying degrees and alleviated abnormal organelle changes.

[0076] From the above test results, it can be seen that feed additives have a good effect in alleviating the symptoms of mycotoxin poisoning in animals after use.

[0077] Although the above embodiments have been described in detail, they are only a part of the embodiments of the present invention, not all of them. People can also obtain other embodiments based on this embodiment without inventiveness, and these embodiments all fall within the scope of protection of the present invention.

Claims

1. A feed additive for alleviating mycotoxin poisoning in animals, characterized by: The components of the feed additive include, by weight, 25 to 30 parts of attapulgite, 25 to 30 parts of montmorillonite, 25 to 30 parts of yeast cell walls, 2 to 5 parts of vitamin E, 5 to 10 parts of taurine and 5 to 10 parts of lipoic acid.

2. The feed additive according to claim 1, wherein: The components of the feed additive include, by weight, 25 parts of attapulgite, 25 parts of montmorillonite, 25 parts of yeast cell walls, 5 parts of vitamin E, 10 parts of taurine and 10 parts of lipoic acid.

3. The feed additive according to claim 2, wherein: The effective mineral mass percentage of the attapulgite is ≥80%; The effective mineral mass percentage of the montmorillonite is ≥90%; The mass percentage of the effective components of the yeast cell wall is ≥78%, of which the mass percentage of β-glucan is ≥60% and the mass percentage of mannooligosaccharide is ≥18%; The mass percentage of the active ingredient of vitamin E is ≥93%; The effective ingredient mass percentage of taurine is ≥98%; The effective component mass percentage of the lipoic acid is ≥98%.

4. A method for preparing the feed additive according to any one of claims 1 to 3, characterized in that: The following steps are involved: (1) Weigh attapulgite, montmorillonite, yeast cell wall, vitamin E, taurine, and lipoic acid according to the above-mentioned parts by weight; (2) mixing the weighed attapulgite, montmorillonite and yeast cell walls; (3) Add the weighed vitamin E, taurine and lipoic acid and mix them to obtain a feed additive for alleviating animal mycotoxin poisoning.

5. Use of the feed additive according to any one of claims 1 to 3 in preparing feed for alleviating mycotoxin poisoning in animals.

6. A feed for alleviating mycotoxin poisoning in animals, characterized by: The feed comprises the feed additive according to any one of claims 1 to 3.

7. The feed according to claim 6, characterized in that: The feed also includes a basic diet and a feed additive, wherein the amount of the feed additive added is 0.1% to 0.2% of the total amount of the basic diet.

8. The feed according to claim 7, characterized in that: The amount of the feed additive added is 0.2% of the total amount of the basic diet.

9. Use of the feed additive according to any one of claims 1 to 3 or the feed according to any one of claims 6 to 8 in promoting the growth of meat ducks poisoned by mycotoxins.

10. Use of the feed additive according to any one of claims 1 to 3 or the feed according to any one of claims 6 to 8 for alleviating poisoning symptoms of meat ducks poisoned by mycotoxins.

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