Primer group and kit for simultaneously detecting multiple pathogenic fungi

Abstract

The invention discloses a primer group and kit for simultaneously detecting various pathogenic fungi. The primer group comprises 14 pairs of primers, and the primers can perform specific multiplex amplification on DNA sequences of 14 target fungi, wherein the target fungi comprise candida albicans, candida tropicalis, candida glabrata, candida parapsilosis, candida krusei, aspergillus fumigatus, aspergillus terreus, trichophyton mentagrophytes, trichophyton rubrum, epidermophytom floccosum, sporothrix schenckii, microsporum caninum, fonsecaea pedrosoi and malassezia fufur. The kit adopts a multiple fluorescence labeling PCR amplification technology for multiplex amplification, multiple common pathogenic fungi can be identified at the same time through a one-time reaction, the detection efficiency is high, the speed is high, and the cost is low; in addition, interspecific and intraspecific comparison can be performed on sequences in a fungus ITS or beta-tubulin database, an intraspecific conserved region is selected to design an amplification primer, and very strong species specificity is achieved. The whole detection process can be completed within 2-3 hours, the detection speed ishigh, and the requirement for rapid detection of clinical samples can be met.

Description

technical field [0001] The disclosure belongs to the technical field of microbial detection, and in particular relates to a primer set for simultaneous detection of multiple pathogenic fungi, a kit containing the primer set, and applications thereof. Background technique [0002] Mycosis is a general term for a class of diseases caused by pathogenic fungi. Common pathogenic fungi include Candida, Aspergillus, Trichophyton, Epidermophyton flocculus, Sporothrix, Microsporum canis, Bacillus, Malassezia, etc. According to the different parts of the infected body, it can be divided into superficial mycosis and deep mycosis. The pathogenic fungi of superficial mycosis are mostly ringworm, Microsporum canis and Malassezia, etc., which generally invade human skin, nails and hair. Although there are few fatal cases, the incidence rate is high and easy to develop. Recurrence; the pathogenic fungi of deep mycoses are mostly Candida, Aspergillus, Sporothrix, and Germinal bacteria, etc...

AI Technical Summary

Problems solved by technology

Ordinary PCR and fluorescent quantitative PCR technology can quickly detect trace fungi with high specificity, but only one or several pathogenic fungi can be detected in one experiment, and the detection throughput is low; although gene chip technology has high detection throughput, However, the operation is cumbersome and the detection takes a long time

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