Microbiological feed additive for adjusting energy metabolism impairment of flocks and herds during perinatal period
A technology of microbial feed and energy metabolism, applied in the field of feed additive production, can solve the problems of large selenium, low utilization rate and high bioavailability, and achieve the effects of improving production performance, increasing appetite and high potency
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Embodiment 1
[0031] (1) Strains
[0032] Candida krusei (Candida krusei): purchased from China Industrial Microorganism Culture Collection and Management Center, the strain code is CICC1722.
[0033] Candida utilis (Candida utilis): purchased from the Microbial Culture Collection Center of the Institute of Microbiology, Chinese Academy of Sciences, with the strain code AS2.1180.
[0034] Streptococcus Thermophilus: purchased from the Microbial Culture Collection Center of the Institute of Microbiology, Chinese Academy of Sciences, with the strain code AS1.2471.
[0035] (2) Activation of strains and acquisition of seed solution
[0036] Slope activation medium mass ratio: Candida krusei activation medium (CM0357): 40g glucose, 2g urea, 4g yeast extract, 20g agar, 1000ml water, pH4.5, sterilized by high-pressure steam at 115°C for 15min, for later use ; Candida utilis, Streptococcus thermophilus activation medium (YPD): 20g glucose, 5g yeast extract, 10g peptone, 20g agar, 1000ml water, p...
Embodiment 2
[0049] (1) Strains
[0050] Candida krusei (Candida krusei): purchased from China Industrial Microorganism Culture Collection and Management Center, the strain code is CICC1722.
[0051] Candida utilis (Candida utilis): purchased from the Microbial Culture Collection Center of the Institute of Microbiology, Chinese Academy of Sciences, with the strain code AS2.1180.
[0052] Streptococcus Thermophilus: purchased from the Microbial Culture Collection Center of the Institute of Microbiology, Chinese Academy of Sciences, with the strain code AS1.2471.
[0053] (2) Activation of strains and acquisition of seed solution
[0054] Slope activation medium mass ratio: Candida krusei activation medium (CM0357): 40g glucose, 2g urea, 4g yeast extract, 20g agar, 1000ml water, pH4.5, sterilized by high-pressure steam at 115°C for 15min, for later use ; Candida utilis, Streptococcus thermophilus activation medium (YPD): 20g glucose, 5g yeast extract, 10g peptone, 20g agar, 1000ml water, p...
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