Sonodynamic liposomal material, preparation method and application thereof in preparing pharmorubicin composite liposomal

A technology of epirubicin and compound lipids, applied in the field of medicine, can solve the problems of low tumor cell selectivity, increased drug efficacy, and increased toxicity, and achieve the effects of simple operation methods, reduced toxic and side effects, and low cost

Inactive Publication Date: 2012-09-19
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] The object of the present invention is to provide a kind of sonodynamic liposome material, preparation method and its application in preparing epirubicin complex liposome, the liposome material of the present invention combines existing liposome technology and sonodynamic The combination of dynamic therapy effectively solves the problem that traditional liposomes have low selectivity for tumor cells, which leads to increased drug efficacy and increased toxicity during treatment, and can meet the clinical needs of high efficiency and low toxicity for clinical treatment; at the same time, The present invention also improves the existing liposome preparation method, and obtains a liposome material preparation method that is easy to operate and control and easy to produce

Method used

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  • Sonodynamic liposomal material, preparation method and application thereof in preparing pharmorubicin composite liposomal
  • Sonodynamic liposomal material, preparation method and application thereof in preparing pharmorubicin composite liposomal
  • Sonodynamic liposomal material, preparation method and application thereof in preparing pharmorubicin composite liposomal

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Embodiment 1

[0035] (1) Prepare complex liposomes by combining film dispersion-pH gradient method, dissolve 30mg lecithin, 10mg cholesterol and 5mg chlorin e4 in chloroform to obtain a mixture, and place the mixture in a rotary evaporator under normal pressure Rotate and mix for 5 minutes to fully mix chlorin e4 and lecithin evenly, then vacuumize the rotary evaporator to 0.09Mpa and perform rotary evaporation to evaporate the chloroform to dry up a white translucent film. The rotary evaporation temperature is 35 ℃, add 3ml of citrate buffer solution with a temperature of 35℃ and a pH of 4 to the white translucent film, then add 0.5ml of surfactant Tween 20, then rotate and mix at 40℃ for 2 hours Hydrate the white translucent film, then turn it into a constant temperature magnetic stirrer and stir at 1500 rpm for 30 minutes. After stirring, use a probe ultrasonic machine for ultrasonic treatment for 10 minutes with an ultrasonic power of 450 watts to obtain liposome materials. See figure ...

Embodiment 2

[0038] (1) The complex liposomes were prepared by combining thin film dispersion-pH gradient method, 40mg lecithin, 10mg cholesterol and 5mg chlorin e4 were dissolved in chloroform to obtain a mixture, and the mixture was placed in a rotary evaporator for normal Press and rotate and mix for 10 minutes to fully mix chlorin e4 and lecithin evenly, then vacuumize the rotary evaporator to 0.09Mpa and then carry out rotary evaporation to evaporate the chloroform to dry up a white translucent film. The rotary evaporation temperature is 37°C, add 3.5ml of citrate buffer solution with a temperature of 38°C and a pH of 4 to the white translucent film, then add 0.7ml of surfactant Tween 40, then rotate and mix at 50°C under normal pressure Hydrate the white translucent film in 1 hour, then transfer to a constant temperature magnetic stirrer and stir at 1500 rpm for 30 minutes, after stirring, use a probe ultrasonic machine for ultrasonic treatment for 5 minutes, with an ultrasonic power ...

Embodiment 3

[0041] (1) Prepare complex liposomes by combining thin film dispersion-pH gradient method, dissolve 20mg lecithin, 10mg cholesterol and 5mg chlorin e6 in chloroform to obtain a mixture, and place the mixture in a rotary evaporator under normal pressure Rotate and mix for 15 minutes to fully mix chlorin e6 and lecithin evenly, then vacuumize the rotary evaporator to 0.09Mpa and perform rotary evaporation to evaporate chloroform to obtain a white translucent film. The rotary evaporation temperature is 40 ℃, add 4mL of citrate buffer solution with a temperature of 40℃ and a pH of 5 to the white translucent film, then add 1mL of surfactant Tween 60, and then rotate and mix at 60℃ for 1.5 hours under normal pressure to make the white The translucent membrane was hydrated, then transferred to a constant temperature magnetic stirrer and stirred at 1500 rpm for 30 minutes, after stirring, it was ultrasonically treated with a probe ultrasonic machine for 15 minutes, with an ultrasonic p...

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Abstract

The invention provides a sonodynamic liposomal material, a preparation method and an application thereof in preparing pharmorubicin composite liposomal. The liposomal material comprises 10-40mg of lecithin, 10mg of cholesterol, 5mg of chlorin, 3-5mL of saline citrate buffer solution of which the pH is 4-5 and 0.1-1mL of surfactant. The sonodynamic liposomal material mainly relates to the composite liposomal of pharmorubicin and sonochemical agent chlorin. The preparation method comprises the following steps of: combining the pharmorubicin and the chlorin into the lecithin with a film dispersion method-pH gradient method to form the composite liposomal; and better targeting the pharmorubicin composite liposomal into a tumor tissue to dually kill a tumor cell. The sonodynamic liposomal material is used for treating various tumors and has a good development prospect.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a sonodynamic liposome material, a preparation method and its application in preparing epirubicin complex liposomes. Background technique [0002] Epirubicin is an anthracycline anticancer drug. Its main mechanism of action is to directly intercalate between DNA base pairs, interfere with the transcription process, prevent the formation of mRNA, and thereby inhibit the synthesis of DNA and RNA. has an effect. It is a cell cycle non-specific drug. Epirubicin acts on both the cell membrane and the transport system, but most importantly the nucleus. In addition, epirubicin also inhibits topoisomerase. Epirubicin is an isomer of doxorubicin, and its antitumor effect is similar to that of doxorubicin, but compared with doxorubicin, epirubicin has faster clearance in vivo, higher lipophilicity, and lower toxicity It is mainly used for the chemotherapy of liver cancer, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/127A61K31/704A61K41/00A61K47/28A61K47/34A61P35/00
Inventor 代志军仵文英李莎高洁徐晓娜赵暖暖
Owner XI AN JIAOTONG UNIV
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