Method for testing photodynamic therapy assisted by operative treatment on mouse mammary tumor cell

Abstract

The invention discloses a method for testing photodynamic therapy assisted by operative treatment on mouse mammary tumor cells. The method comprises the following steps: preparing a fluorescent nanoparticle solution; culturing the mouse mammary tumor cells; inoculating the mouse mammary tumor cells at oxter of mice of 6 weeks; two weeks later, dividing the mice into a control group, a mere exairesis group, a mere photodynamic therapy group and an exairesis and photodynamic therapy group; killing residue tumor with operation together with photodynamic therapy, namely, 12 hours before operation, injecting the prepared fluorescent nanoparticle solution into the mice of different groups, irradiating with white light for 15 minutes, and respectively monitoring sizes of tumor; after confirming that the tumor of the experiment groups is eliminated, inoculating same tumor cells at backs of the mice of different groups, and continuously monitoring development of the tumor inoculated later. The method has the advantages that a photosensitizer with a property of aggregation-induced emission, and exairesis, are combined, and the defects of radical cure limit, slow treatment and postoperative recurrence of a conventional method can be solved.

Description

technical field [0001] The invention relates to assisted surgical treatment of cancer cells, in particular to a test method for photodynamic therapy assisted surgical treatment of mouse breast cancer cells. Background technique [0002] Cancer has biological characteristics such as abnormal cell differentiation and proliferation, uncontrolled growth, invasion and metastasis, and its occurrence is a multi-factor and multi-step complex process. During the surgical operation, the surgical operation mainly judges the scope and margin of tumor resection based on the color, shape, and texture of the tissue observed with the naked eye. If the tumor cells are completely removed by surgery, the cancer can be cured immediately and quickly. Surgical resection remains the treatment of choice for early or earlier solid tumors. However, if some tumors often cause accidental damage or tumor residues due to tumor infiltration and abnormal surrounding structures, they may not even be recog...

AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is to address the above existing problems and technical analysis, to provide a test method for photodynamic therapy assisted surgical treatment of breast cancer cells in mice, which combines photosensitizers with aggregation-induced luminescent properties and surgical excision, which solves the problem of traditional methods. Disadvantages of irreversible limitations, slow treatment, and postoperative recurrence

Images