Technology for producing triptodiolide with leaf of tripterygium

A technology of triptolide and vine leaves, which is applied in the technical field of producing triptolide from tripterygium leaves, can solve the problems of consumption, high extraction cost, complicated steps, etc., and achieve improved extraction yield, reduced process steps, The effect of reducing production costs

Active Publication Date: 2010-06-23
福建省汉堂生物制药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The second, boiling extraction process: currently mostly used in laboratories, through boiling alcohol precipitation to remove impurities, not only consumes a lot of ethanol, but also takes away more than 20% of the active ingredients, affecting the yield and production cost
The third method is the natural precipitation method of boiled liquid: the production cycle of this method is long, and the clarified water sedimentation liquid cannot be filtered out, which affects the yield and production cycle of the extraction process
The fourth method is the freezing natural sedimentation method. Although the sedimentation effect of this method is better, it consumes a lot of energy and the production cost is high.
Fifth, the concentrated solution boiled in water is degreased and then extracted: this method not only consumes a large amount of petroleum ether, but also causes unsafe factors in production
But this method has the following disadvantages: it is high cost and relatively unsafe to extract with ethanol; the extraction concentrate is extracted with ethyl acetate, and the extract is extracted with petroleum ether---methanol, and the three solvents used are all flammable Explosive and harmful poisonous (methanol) substances, the extraction method laboratory test is acceptable, industrial production safety hazard is great; crude A element is removed by quaternary two-phase method, and then refined by high-speed countercurrent chromatography, there are many steps. One-step quaternary two-phase method, the steps are complicated, not suitable for industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A process for producing triptolide from tripterygium twig leaves, including dry leaf grinding→boiling→first concentration→centrifugation to remove impurities→chloroform extraction→second concentration→extraction on a silica gel column→third concentration →Second loading to the column→Fourth concentration→Crystalization→Recrystallization→Pure product→Check storage steps.

[0019] Grinding of dry leaves: the leaves of Tripterygium wilfordii grown for more than 6 years are harvested from July to August every year and dried in the sun or at 50-60°C, with a moisture content of less than 8%, and crushed to 30-40 meshes.

[0020] Boil in water: take a certain amount of dry leaf powder, add 6 times of water and boil for 3 hours, add 6 times of water for the second time and boil for 2 hours, add 6 times of water for the third time and boil for 2 hours, the temperature is 98°C, and the pressure is normal.

[0021] Concentration for the first time: Combine and concentrate the thre...

Embodiment 2

[0032] Grinding of dry leaves: the leaves of Tripterygium wilfordii grown for more than 6 years are harvested from July to August every year and dried in the sun or at 50-60°C, with a moisture content of less than 8%, and crushed to 30-40 meshes.

[0033] Boil in water: take a certain amount of dry leaf powder, add 10 times of water and boil for 3 hours, add 8 times of water for the second time and boil for 2 hours, add 8 times of water for the third time and boil for 2 hours, the temperature is 100°C, and the pressure is normal.

[0034] Concentration for the first time: Combine and concentrate the filtrates boiled three times to twice the volume of the dry leaves to obtain a concentrated solution.

[0035] Centrifugal impurity removal: the concentrated liquid obtained after concentration is separated in a high-speed centrifuge with a speed of 20,000 times / min to obtain a clarified liquid from solids accounting for about 5% of the concentrated liquid. The solids include protei...

Embodiment 3

[0044] Grinding of dry leaves: the leaves of Tripterygium wilfordii grown for more than 6 years are harvested from July to August every year and dried in the sun or at 50-60°C, with a moisture content of less than 8%, and crushed to 30-40 meshes.

[0045] Boiling: Take a certain amount of dry leaf powder, add 8 times of water and boil for 3 hours, add 7 times of water for the second time and boil for 2 hours, add 7 times of water for the third time and boil for 2 hours, the temperature is 99°C, and the pressure is normal.

[0046] Concentration for the first time: Combine and concentrate the filtrates boiled three times to 1.5 times the volume of the dry leaves to obtain a concentrated solution.

[0047] Centrifugal impurity removal: the concentrated liquid obtained after concentration is separated in a high-speed centrifuge with a rotating speed of 15,000 times / min to obtain a clarified liquid, which includes protein, polysaccharide, wax, and fine solid particles.

[0048] Ch...

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PUM

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Abstract

The invention relates to a technology for extracting active ingredient of plant, in particular to a technology for producing triptodiolide with leaf of tripterygium, which comprises the following steps: grinding dry leaf-water boiling-first concentrating-centrifugally removing impurity-extracting chloroform-second concentrating-extracting on silica gel column-third concentrating-second extracting on silica gel column-fourth concentrating-crystallizing-recrystallizing-purifying-inspecting to store. The invention has the advantages of simple process flow, safe production and high yield, and is suitable for industrial production.

Description

【Technical field】 [0001] The invention relates to a process for extracting effective plant components, in particular to a process for producing triptolide from tripterygium twig leaves. 【Background technique】 [0002] Tripterygium Wilfordii (Tripterygium Wilfordii Hook f. TWHF for short) is a woody vine of the genus Tripterygium in the family Euonymus. Due to its high toxicity, it is used in the folk for killing insects and rats. However, as a traditional Chinese medicine, it has been used in China for hundreds of years. It has anti-inflammatory, anti-tumor, anti-fertility and other activities. Its chemical components are mainly diterpenes, triterpenes, sesquiterpenes, alkaloids, etc. . Among them, diterpene lactone is the main active ingredient. Triptolide, also known as triptolide, belongs to diterpene lactone and is considered to be the most important physiologically active ingredient. Therefore, triptolide is extracted from Triptolide Ester alcohols are of great value...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J73/00
Inventor 魏佰兴
Owner 福建省汉堂生物制药股份有限公司
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