62 results about "Spermatogeny" patented technology

The invention relates to the technical field of biological medicines, in particular to an umbilical cord mesenchymal stem cell exosome and application of the umbilical cord mesenchymal stem cell exosome in preparation of a medicine for treating male infertility caused by hidden testis. The prepared high-concentration umbilical cord mesenchymal stem cell exosome suspension preparation is directly used for treating cultured spermatogonial cells and spermatoblasts in vitro, is injected into the testis of a mouse, and is used for improving the seminiferous tubule and spermatogenic microenvironment of testis tissue and promoting division and proliferation of the spermatogonial cells and the spermatoblasts. The method can effectively reverse oligospermia and azoospermia caused by the hidden testis, and compared with a model without intervention of treatment means, the method can effectively promote increase and even regeneration of sperms in the testis.

The invention discloses a traditional Chinese medicine compound oral preparation for treating male oligoasthenospermia, and belongs to the technical field of traditional Chinese medicine preparations. The rhizoma polygonati processed with wine, the prepared rehmannia root and the like are used for nourishing the liver and kidney to consolidate congenital, the codonopsis pilosula, the astragalus membranaceus and the like are used for invigorating spleen and replenishing qi to complement postnatal, the fried cowherb seed is used for activating blood circulation, and the salted semen plantaginis is used for promoting diuresis, so that the problems of insufficient spermatogenesis and low vitality caused by deficiency of the spleen and the kidney are fundamentally solved. Experiments and clinical researches show that the preparation can significantly improve the sperm quantity, activity and activity rate of oligoasthenospermia model mice and patients, the clinical total effective rate reaches 90%, the pregnancy rate reaches 53.3%, and the curative effect is superior to that of Wuzi Yanzong pills in a control group. The action mechanism covers regulation of blood lipid metabolism, improvement of hypothalamus-pituitary-gonad axis functions, repair of testicular seminiferous tubule structural damage and reduction of lipid deposition, and meanwhile, oxidative stress and inflammatory injury are relieved and spermatogenic cell functions are protected by regulating and controlling a PPAR signal channel and inhibiting molecular pathways such as Cga / Sting mediated cell apoptosis.

The invention discloses a spermatogenesis disorder sperm image recognition system based on deep learning, and relates to the technical field of sperm image recognition. The image recognition management center is in communication connection with an image acquisition module, a sperm positioning module, a feature extraction module, a motion feature analysis module, a sperm form classification module, a sperm state comprehensive recognition module and a result display feedback module, and all the modules are in electric signal connection. According to the method, morphology and motion features are learned and extracted from a large amount of sperm image data through a deep learning technology, the accuracy of sperm classification is improved, the time and errors of manual operation are remarkably reduced, meanwhile, fine differences of sperms can be accurately recognized, the diagnosis efficiency is greatly improved, and the method is suitable for popularization and application. Therefore, a doctor can obtain a more accurate diagnosis result in a shorter time, and a more timely treatment suggestion is provided for a patient.

The invention discloses an application of luteolin in improving the reproductive performance of cocks. Researches show that in the breeding process of the cocks, the breeding performance of the cocks can be effectively improved by feeding the luteolin. Test data show that the luteolin can significantly improve the cock semen collection amount, improve the sperm density and sperm motility, significantly improve the diameter of a fine sperm tube and a spermatogenic cell matrix layer, and artificial insemination of the test cock semen improves the hatching egg fertilization rate, the hatching rate and the healthy chick rate. Therefore, the luteolin has important significance in research and development of related products for improving the reproductive performance of the cocks, and the luteolin has great potential in preparation of medicines, feeds or feed additives for improving the reproductive performance of the cocks.

The invention relates to the technical field of biology, in particular to application of rosa roxburghii tratt active extract in preparation of a medicine for treating dyszoospermia. The extract provided by the invention is prepared from roxburgh rose fruits or roxburgh rose pomace through extraction and enrichment, the raw material resources are rich, the price is low, and the toxic and side effects are low. Animal experiments show that the roxburgh rose active extract has the activity of improving dyszoospermia, and the roxburgh rose active extract can obviously increase the sperm concentration and motility rate of mice with dyszoospermia, reduce the malformation rate and obviously increase the thickness of spermatogenic epithelium, the number of supporting cells and the number of interstitial cells of testicular tissues of the mice with dyszoospermia. WB detection analysis shows that the action way of improving the dyszoospermia by the roxburgh rose active extract is to inhibit activation of an oxidative stress Keap1 / Nrf2 signal channel. The rosa roxburghii tratt active extract has a new function of improving dyszoospermia and can be applied as a medicine for improving dyszoospermia.

This invention provides a biomarker for spermatogenesis disorders and its application, wherein the biomarker is the MNDA molecule. This application characterizes the functional abnormalities or spermatogenesis disorders of Sertoli cells by detecting the expression level of MNDA molecules on cells in testicular tissue sections, and can therefore be used for the clinical diagnosis of non-obstructive azoospermia.

The invention relates to the technical field of biomedicine, in particular to a construction method and application of a testis organ capable of differentiating sperms in vitro. The polypeptide hydrogel is used as a three-dimensional scaffold for testicular cell growth, and meanwhile, the testicular cell proliferation promoting liquid and the testicular cell differentiation promoting liquid are added in different culture stages, so that the construction of testicular organs is effectively realized. The obtained testis organs comprise testis seminiferous tubule structures consistent with those in vivo and various testis cells with the same arrangement mode; and in addition, spermatogonium can be successfully induced to differentiate deformed sperms in vitro. The testicular organ construction method provided by the invention is short in period, high in sperm differentiation success rate, simple and convenient to operate and low in cost. The constructed testis organ can be applied to screening of drugs or toxicants, testis ectogenesis models and clinical diagnosis and treatment, and has a wide application prospect and an important scientific value.

The invention belongs to the technical field of biological medicines, and particularly discloses application of roxburgh rose glycoside in preparation of a medicine for improving dyszoospermia. Pharmacodynamic verification is carried out by constructing various animal models with dyszoospermia, and it is found that the roxburgh rose glycoside can significantly improve reproductive function indexes, for example, the roxburgh rose glycoside can significantly increase the density of testicular interstitial cells, the thickness of spermatogenic epithelium and the density of sperms in epididymis lumens. As a main active component of a medicinal and edible plant roxburgh rose, the roxburgh rose glycoside has good biological safety and human body tolerance. The new application of the roxburgh rose glycoside in the field of reproductive medicine is disclosed for the first time, a novel candidate medicine is provided for treating dyszoospermia, and meanwhile, an application system of the roxburgh rose glycoside in functional health care products and healthy food additives is constructed.

The invention provides a composition for promoting proliferation of mesenchymal stem cells and / or relieving spermatogonial cell injury as well as a preparation method and application of the composition, and belongs to the technical field of traditional Chinese medicine compositions. The composition is prepared from the following raw materials in parts by weight: 10-20 parts of ginseng, 5-15 parts of maca, 5-10 parts of oyster peptide, 5-15 parts of sika deer penis, 3-8 parts of sika deer blood, 3-8 parts of fresh ginger, 5-8 parts of herba cistanche, 4-10 parts of sea cucumber peptide, 4-8 parts of rhizoma polygonati, 5-10 parts of burdock root, 2-5 parts of fructus lycii powder and 1-3 parts of folium cortex eucommiae. The composition is prepared into a traditional Chinese medicine preparation by adopting a superfine grinding process, and the traditional Chinese medicine preparation has the effects of promoting proliferation of mesenchymal stem cells (MSCs), repairing spermatogonial cell injury caused by oxidative stress (such as chemotherapy and radiation) or aging (such as age-related), reducing the ROS level, improving spermatogenic dysfunction, assisting in treating male infertility and delaying senescence of a reproductive system and a whole body.

The invention discloses application of exosome-like nanoparticles derived from fructus lycii to preparation of a medicine for preventing or treating oligospermia and / or asthenospermia. Sterile, stable and high-purity fructus lycii exosome-like nanoparticles (LBELNs) are obtained by adopting a differential centrifugation-suction filtration-ultra-high-speed centrifugation method, the physiological efficacy of the LBELNs is researched, and it is found that the LBELNs can significantly improve the oligoasthenospermia disease activity index of male mice induced by cyclophosphamide (CTX); the sperm quantity is obviously increased, the sperm malformation is improved, the sperm motility is improved, and the sex hormone level is recovered. The LBELNs can effectively relieve spermatogenic dysfunction of testicular tissues, the sperm differentiation and formation process is obviously improved, the total number of sperms is increased, and the treatment effect is more obvious along with increase of the dosage of the LBELNs. The invention has potential in preparation of drugs for preventing or treating oligoasthenospermia, and provides a new strategy and treatment method for treating oligoasthenospermia.

The invention provides oleanolic acid, and a preparation method of the oleanolic acid comprises the following steps: S1, dissolving 5g of arginic acid in 500ml of dichloromethane, adding 7g of pyridinium dichlorochromate, stirring at room temperature for 18 hours, and detecting the reaction degree by thin-layer chromatography; s2, after the reaction is completed and vacuum concentration is carried out, a sample is mixed with 200-300-mesh silica gel, the mixture is volatilized to be dry and then placed on a silica gel column, column chromatography separation is carried out, petroleum ether / ethyl acetate is selected as a mobile phase, 2.87 g of oleanolic acid is obtained, the molecular formula is C30H46O3, and the yield is 57.6%. The oleanolic acid provided by the invention can be used for preparing medicines for treating oligospermia and medicines for improving dyszoospermia. The oleanolic acid provided by the invention is a natural product or an artificially synthesized product, is low in toxicity and small in side effect, and has good effects of improving dyszoospermia and promoting fertility.

The invention provides a marker for evaluating the spermatogenic capability of a Kalman syndrome patient after spermatogenic induction or spermatogenic treatment. The marker comprises GLIPR1L1 (Q6UWM5) and other six markers. Compared with the prior art, the invention has the following advantages: the invention provides the marker for evaluating the spermatogenic ability of the Kalman syndrome patient after spermatogenic induction or spermatogenic treatment, and the marker can accurately diagnose whether the KS patient successfully generates spermatogenic; the ROC curve verifies that the AUC value is 1, and the visualization of the sample dimension reduction graph and the confusion matrix has a remarkable sample classification effect; the compliance of the patient is improved, clear spermatogenesis judgment can be given to guide clinical treatment and assist reproduction technology, and the fertility of the patient is improved.

This invention discloses a Fam205a1 Methods for constructing and applying gene knockout mouse models. The construction method utilizes CRISPR / Cas9 gene editing technology, targeting mice. Fam205a1 Gene-designed specific sgRNAs for knockout Fam205a1 The regions of exons 1 through 4 of the gene are obtained. Fam205a1 Gene knockout mice. Male mice in this model exhibit infertility, specifically characterized by impaired spermatogenesis, reduced sperm motility, abnormal sperm morphology (particularly head and acrosome defects), and spermatogenic cell apoptosis. However, the weight and appearance of their testes and epididymis show no significant difference from the wild type. The mouse model constructed in this invention has stable genetic traits and a well-defined phenotype, making it an ideal animal model for studying the mechanisms of idiopathic male infertility, screening drugs for treating male infertility, or developing male contraceptives.

The invention relates to the technical field of biological medicines, and discloses an application of a plant extract composition in preparation of a medicine for improving DNA damage of testicular spermatogenic cells, and the plant extract contains a herba epimedii extract and a rhizoma polygonati extract. The invention provides new application of the composition containing the herba epimedii extract and the rhizoma polygonati extract in preparation of the medicine for improving DNA damage of the testicular spermatogenic cells, the composition can remarkably improve the DNA damage of the testicular spermatogenic cells of mice caused by cyclophosphamide, recover the multiplication capacity of the mice, inhibit apoptosis of the mice and relieve spermatogenic function damage caused by cyclophosphamide, and the composition can be used for preparing the medicine for improving the DNA damage of the testicular spermatogenic cells of the mice. And the combined administration shows a synergistic effect instead of a simple additive effect, and the drug effect is obviously superior to that of any one of the epimedium extract and the rhizoma polygonati extract which are independently administered.

The invention belongs to the field of basic medical research, and particularly relates to a testicular toxicity animal model establishing method which comprises the following steps: performing intragastric administration on male rats with a preparation containing podophyllotoxin according to the dosage of 15 mg / kg per day; the medicine is continuously administrated for 4 days; according to the method, the rat can be induced to generate testicular toxicity phenotypes, and the phenotypes at least include that the number of sperms is remarkably reduced, the activity of the sperms is reduced, the testosterone level of serum is abnormally increased, and spermatogenic tubules in testicular tissue are irregular in shape and spermatogenic epithelium is thinned.

The invention discloses application of tremella aurantialba polysaccharide in preparation of a medicine for treating male immunological dyszoospermia, and belongs to the field of biological medicine. The invention provides a new application of tremella aurantialba polysaccharide, and experiments prove that the tremella aurantialba polysaccharide can improve dyszoospermia induced by cyclophosphamide, significantly increase the number of sperms of mice, reduce the abnormal rate of sperms and promote secretion of testosterone. According to the invention, cyclophosphamide is adopted to construct a male mouse dyszoospermia model, tremella aurantialba polysaccharides extracted in different manners are subjected to intragastric administration, the action mechanism of NAPs for improving mouse dyszoospermia is researched, the mechanism of NAPs for treating spermatogenesis is disclosed, and a basis is provided for clinical application.

The application belongs to the technical field of biology, and discloses a method for activating an ASXL2-PRC2 axis, wherein adenovirus is injected into the seminiferous tubule of an organism through an outgoing pipe to activate the expression of endogenous EZH2 and spermatocyte-specific ASXL2 in the organism. The method can be used for reducing impaired sperm motility, increasing sperm penetration and tubulin polymerization, inhibiting abnormal spermatogenesis, down-regulating the transcription level of a TZ marker, increasing the enrichment of SUZ12, EED and H3K27me3 in CEP162 and CEP290 promoters under hypoxic conditions, and slowing down hypoxia-induced abnormal spermatogenesis and spermatocyte activation of the ASXL2-PRC2 axis in the germ cell line of the organism.

The invention relates to the technical field of health-care food, and discloses a food composition for improving sperm concentration and a preparation method thereof, the food composition for improving sperm concentration comprises hippocampus, fresh oysters, mulberries, Chinese chive seeds, Chinese wolfberry fruits, algae and nereis. The natural food is used as the raw material, and the spirulina and the nereis are especially added, and the two components have relatively strong antioxidant ability, can effectively remove free radicals in the mouse body, reduce testis injury of the mouse, and further improve the sperm concentration of the mouse. Through intragastric administration of the food composition in the treatment period, it is found that the sperm concentration of a modeling mouse is increased by 2.9 times compared with that of a modeling mouse subjected to intragastric administration with normal saline, and the sperm concentration increasing effect is far higher than that of spermatogenic capsules widely sold in the market. Besides, the food composition is simple in preparation process, the adopted raw materials are all from natural food, and the food composition has the advantages of small toxic and side effects, wide and lasting effect and the like, and is widely distributed in nature, rich in resources and low in production cost.

The invention relates to a compound microbial agent and application thereof, belongs to the technical field of microorganisms, and particularly discloses application of mixed fermented milk in a product for improving male functions. According to the application, the new application of the mixed fermented milk of the rhamnosus lactobacillus B6 and the streptococcus thermophilus XJ53 (CGMCC No.17323) in the aspect of improving male functions is disclosed for the first time, and the new application is specifically characterized by improving male fertility desire, reproductive capacity, seminiferous tubule dysfunction and spermatogenesis, testis oxidative stress activity, testis testosterone synthesis and sperm quality; the strain has a potential value of producing a gain substance for improving the male function by fermenting skimmed milk, and also prompts that the fermented milk has a potential application prospect for enhancing the male function.

The invention provides a pyrrolidone tricyclic compound and a preparation method thereof, and application of the pyrrolidone tricyclic compound in preparation of drugs for treating neurodegenerative diseases, especially antidepressant drugs. The structural formula of the pyrrolidone tricyclic compound is as shown in general formulas A and 13. Experimental studies on in-vitro PC12 cell injury protection activity, in-vivo mouse anti-depression activity, hERG potassium channel inhibition and mouse head shaking show that the compound disclosed by the invention can be used for preparing neurodegenerative diseases, especially quickly-acting anti-depression drugs, and has the remarkable advantage of improving the spermatogenesis function.

The invention belongs to the technical field of microorganisms, and particularly discloses application of lactobacillus rhamnosus fermented milk in a product for improving male functions. According to the application, the new application of the lactobacillus rhamnosus B6 (CGMCC No.13310) fermented milk in the aspect of improving male functions is disclosed for the first time, and the new application is specifically characterized by improving male fertility desire, reproductive capacity, seminiferous tubule dysfunction and spermatogenesis, testis oxidative stress activity, testis testosterone synthesis and sperm quality; the strain has a potential value of producing a gain substance for improving the male function by fermenting skimmed milk, and also prompts that the fermented milk has a potential application prospect for enhancing the male function.

The invention provides development of a spermatogonial-targeting lipid nanomaterial and application of the spermatogonial-targeting lipid nanomaterial in treatment of male infertility, relates to the technical field of biomedicine, and has the technical key points that the lipid nanomaterial and a preparation method thereof are provided, mRNA is delivered to spermatogonial by using the lipid nanomaterial, and the spermatogonial-targeting lipid nanomaterial is prepared by editing the spermatogonial. And edited sperm cells and sperm cells can be obtained. The invention provides an ionizable phospholipid nanoparticle LNP65, which can be injected through a testicular net to effectively transfer mRNA (messenger ribonucleic acid) to spermatogonium in a mouse testis. The spermatogonium has been successfully edited by this method. In one spermatogenic cycle, a modified sperm from a spermatogonial cell edited by LNP65 is obtained. And after three spermatogenesis cycles, observing that the spermatogonium cells edited by the LNP65 are cleared from the testis. The invention develops a lipid nanomaterial delivery mRNA (messenger Ribonucleic Acid) system of targeted spermatogonium, and provides an applicable carrier for treating azoospermia caused by single gene deletion.

The invention relates to the technical field of micro-fluidic organ chips, and discloses a micro-fluidic testis organ chip and a culture method, the micro-fluidic testis organ chip comprises a PDMS fluid control layer and a PDMS cell culture layer from top to bottom; the at least three culture units are arranged on the PDMS cell culture layer in the axial direction, each culture unit internally comprises three functional areas, the three functional areas are used for testicular sertoli cell culture, spermatogenic cell culture and basement membrane simulation respectively, and the three functional areas are communicated with one another to form a fluid path; the regulation and control part is arranged on the PDMS fluid control layer, and the regulation and control part is used for regulating and controlling fluid paths which are communicated with one another among the three functional areas; the inoculation and collection part is arranged on the PDMS fluid control layer and is used for carrying out cell inoculation and collection in the three functional areas. The in-vivo testis spermatogenesis microenvironment is highly simulated, the limitation of a traditional 2D cell experiment and an animal experiment can be effectively made up, and a new method and platform are provided for analyzing a spermatogenesis disorder mechanism and screening targeted repair drugs.

The invention discloses an application of an Odf3 gene or a protein coded by the Odf3 gene in preparation of a medicine for regulating fertility of male animals, and belongs to the technical field of biological medicines. The AAV2 / 9 shows huge advantages in a testis overexpression mode, and keeps safety and effectiveness under the condition of efficient delivery. The Odf3 delivered by the testis seminiferous tubule through the AAV2 / 9 can be successfully expressed in spermatogenic cells, the sperm motility can be effectively improved, the fertility of the Odf3- / -mouse is saved, and offspring can be generated under the condition of natural mating. The F1-generation heterozygote mouse produced in a gene overexpression mode has the same fertility as that of a common heterozygote mouse.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to sperm-producing decoction as well as a preparation method, application and a product thereof. The sperm-producing decoction is prepared from the following raw materials in parts by weight: 5 to 15 parts of radix salviae miltiorrhizae, 5 to 15 parts of rhizoma chuanxiong, 5 to 15 parts of radix angelicae sinensis, 10 to 25 parts of herba epimedii, 10 to 25 parts of fructus cnidii, 10 to 25 parts of semen cuscutae, 5 to 15 parts of fructus rubi and 5 to 15 parts of fructus schisandrae chinensis. The spermatogenic decoction can improve testis injury caused by an anti-inflammatory and anti-immune drug tripterygium wilfordii hormone, improve oxidative injury of testis tissues, regulate the expression level of antioxidant stress related proteins Nrf2 and HO-1, improve the phenomena of testis diminishment, seminiferous tubule damage, sperm number reduction, sperm motility weakening, testis spermatogenic cell apoptosis and the like, and can be used for preparing the anti-inflammatory and anti-immune drug. And the expression level of apoptosis-related proteins Bcl-2 and Bax of the testicular spermatogenic cells is regulated.

The invention belongs to the technical field of biological medicine, and particularly relates to application of 1, 3-propane diamine or pharmaceutically acceptable salt thereof in preparation of a medicine for preventing or treating male dyszoospermia. In a specific embodiment, the invention relates to application of 1, 3-propane diamine or pharmaceutically acceptable salt thereof in preparation of a medicine for preventing or treating male diabetic dyszoospermia. In experiments, the inventor finds that 1, 3-propane diamine can obviously improve the sperm concentration of diabetic mice and improve the expression quantity of testis spermatogenesis related proteins. According to the application, 1, 3-propane diamine is applied to diabetic dyszoospermia for the first time, an ideal treatment effect is achieved, a new treatment choice is provided for clinical application of 1, 3-propane diamine to treatment of diabetic dyszoospermia, and the application has extremely high application value and social benefits.

The present invention discloses a sperm image recognition system for spermatogenesis disorders based on deep learning, which relates to the field of sperm image recognition technology, including an image recognition management center, wherein the image recognition management center is communicatively connected with an image acquisition module, a sperm positioning module, a feature extraction module, a motion feature analysis module, a sperm morphology classification module, a sperm state comprehensive recognition module, and a result display feedback module, wherein electrical signals are connected between each module. The present invention learns and extracts morphological and motion features from a large amount of sperm image data through deep learning technology, which not only improves the accuracy of sperm classification, but also significantly reduces the time and error of manual operation, and can accurately identify subtle differences in sperm, greatly improving diagnostic efficiency, enabling doctors to obtain more accurate diagnostic results in a shorter time, and provide patients with more timely treatment recommendations.

The application belongs to the technical field of reproductive health, and particularly relates to a new application of a testicular metabolite 2-n-propylthiazolidine-4-carboxylic acid (PTCA), and the 2-n-propylthiazolidine-4-carboxylic acid is used for preparing a drug for treating testicular spermatogenic disorder induced by AFB1. The PTCA can promote the secretion of testosterone of testicular interstitial cells, activate the PI3K / AKT / NRF2 antioxidant signaling pathway in testicular tissue, relieve testicular injury induced by AFB1, and reduce the ROS level in testicular tissue. The application discloses, for the first time, the important role of the PTCA as a new testicular metabolic marker and protective agent in the male reproductive system, provides a new insight into the reproductive toxicity mechanism of AFB1, and lays an experimental foundation for the application of metabolites to the intervention strategy of male spermatogenic disorder.

The application belongs to the technical field of biological medicine, and particularly relates to application of a reagent for inhibiting PPAR alpha expression in preparation of a medicine for treating male infertility diseases, wherein a PPAR alpha inhibitor (GW6471) or an active oxygen scavenger (NAC) is given to male mice exposed to cadmium water, it is found that the number of sperm in a Cd+GW6471 group is higher than that in a Cd+NAC group, the serum testosterone level in the Cd+GW6471 group is also higher than that in the Cd+NAC group, and the results of testicular tissue pathological staining also show that GW6471 can more significantly restore the decrease in the number of mature spermatogenic tubules caused by Cd, GW6471 restores the decrease in the number of SYCP3 positive spermatocytes caused by Cd, so that the sperm production efficiency is restored, and therefore the application of the reagent for inhibiting PPAR alpha expression in preparation of the medicine for treating male infertility diseases is proposed.

The invention relates to the technical field of medical detection, in particular to an antibody combination for detecting spermatogenic cells, a kit and application of the antibody combination. The antibody combination comprises an antibody of a zinc finger transcription factor 4, an antibody of a conjugation complex protein 3 and an antibody of zona pellucida binding protein. Specific recognition and semi-quantitative analysis of spermatogenic cells of all levels in samples are realized through standardized pretreatment, full-automatic immunohistochemical staining and digital image analysis for different types of samples; the method breaks through the technical bottlenecks that the traditional HE staining or Wright-Giemsa staining method is strong in subjectivity, difficult to distinguish cells with similar forms and greatly influenced by cell states and staining effects, and provides an efficient detection means for accurate pathological typing, spermatogenic function evaluation and dynamic curative effect monitoring of male infertility.