79 results about "Spermatogeny" patented technology

The invention relates to Chinese herbal medicine feed additive for stud rams, which mainly solves the problem that the sperm quality of stud rams is reduced due to centralized and high-intensity sperm collection in the prior art. The Chinese herbal medicine feed additive is prepared by crashing and mixing epimedium, actinolite, Chinese dodder seed, raspberry, Chinese magnoliavine fruit, astragalus root and dangshen and can be added into feed for usage. The invention improves ram sexual appetite, prompts spermatogenesis, increases sperm motility, effectively improves reproductive performance, solves the problem that the sperm quality of stud rams is reduced due to centralized and high-intensity sperm collection in the prior art according to spermatogenesis principle.

The present invention provides a group of mouse spermatogenesis genes, which is an assembly of 89 genes in total, wherein the respective genes transcribe mRNAs from which cDNAs having the respective base sequences of SEQ ID NOs:1 to 89 are synthesized; an Scot-t gene mutation and a protamine-2 gene mutation which are human homologues of genes belonging to the group of mouse genes and associated with human male infertility. The invention also provides a group of mouse spermatogenesis genes and various molecular biological materials relating thereto; various molecular biological materials relating to male infertility-associated gene mutations; and various test methods and diagnostic methods using the same.

The invention discloses sperm piRNA (Piwi-interacting Ribonucleic Acid) marker combination related to male reproductive functional disturbance and application thereof. A marker comprises ten piRNAs (mainly comprising piR-1207 and piR-2107), and the sperm piRNA marker combination can be used for detection and prediction of sperm viability. In the invention, sperm can be relatively easily obtained, other tissues are not required, and the detection belongs to non-traumatic examination; sperm piRNA and MitoPLD (Phospholipase D) protein can be used for reflecting pathological and physiological status of a whole spermatogenic process at a molecular level, the accurate level of detection can be increased by taking the sperm piRNA and the MitoPLD protein as auxiliary detection indexes, and a potential target spot is provided for treatment of the male reproductive functional disturbance.

The invention discloses a method for predicting whether sperms exist in the testis of an azoospermia patient or not according to seminal plasma mRNA. The method comprises the following steps of dividing seminal fluid specimens of the azoospermia patient into a testicular sperm group and a testicular azoospermia group by taking a testicular biopsy as a gold standard, determining the difference of exRNA expression profiles of two seminal fluid groups, carrying out comparison with an existing spermatogenic cell transcriptome database, and searching for differentially expressed exRNA capable of judging spermatogenic cell types, which is used for judging whether haploid sperm cells exist in the testis of the azoospermia patient or not. The clinical application of the scheme provided by the invention can greatly improve the sperm taking success rate of the azoospermia patient, and is also helpful for guiding the azoospermia patient to select an economical, painless and effective treatment method to solve the problem of infertility of the azoospermia patient.

Applications of cyanidin-3-O-glucoside in preparation of medicines treating and / or preventing diseases caused by 3-chloropropane-1,2-diol are provided. The cyanidin-3-O-glucoside (C3G) can improve rat ingestion decrease and growth retardation which are caused by 3-MCPD poisoning, can effectively resist rat antioxidant system damage caused by 3-MCPD poisoning, and can activate and adjust BTB structural functions of sustentacular cells through inhibiting an MAPK signaling pathway, thus relieving seminiferous epithelium injuries caused by the 3-MCPD, maintaining stability of a spermatogenic microenvironment, regulating spermatogenic cell proliferation and differentiation, and improving dyszoospermia. In addition, the C3G can improve sperm activity through adjusting sperm energy metabolism related enzymes. The C3G can regulate rat sex hormone secretion disorders caused by 3-MCPD poisoning and can maintain normal levels of sex hormone in serum and androgen in testis inner environments. The applications provide theoretical foundations for 3-MCPD reproduction toxicity nutritional intervention utilizing anthocyanin.

The invention relates to an application of seabuckthorn fruit oil and / or whole fruit oil in preparing medicine promoting the formation of male sperms, preventing the apoptosis of spermatogenic cells or promoting the formation of female ovarian follicles and the maturation of germ cells and treating male and female sterility. By taking the preparation, kidney deficiency and infirmity and various chronic diseases can be recovered to the health status, more effectively, the invention has the functions of promoting the formation of the male sperms and protecting the quantity of the spermatogenic cells, and preventing the apoptosis of spermatogenic cells, and can obviously increase the quantity and the motor ability of the sperms, and increase the weight of spermary, and simultaneously has the functions of promoting the formation of the female ovarian follicles and the maturation of the germ cells, thereby being used as the medicine for the clinical treatment of male and female sterility. Compared with other medicines, the medicine has simple ingredients, belongs to target organ excitant, and has the reversible automatic regulation function of a second messenger, thereby being the medicine with high curative effect, security, and simplification.

The present invention provides a method of producing a non-human vertebrate that harbours germ cells having a desired gene transferred thereto, comprising injecting the desired gene to the testis of a non-human vertebrate wherein no tight junction exists between Sertoli cells to transfer the desired gene to germ cells, so as to obtain a non-human vertebrate that harbours the germ cells having the desired gene transferred thereto. Using the method of the present invention, even in animal species and lines for which in vitro transduction has been difficult to date, it is possible to obtain an individual harbouring germ cells, particularly spermatogonial stem cells, having a desired gene transferred thereto, at extremely high efficiency. Also, the fertility of the male to receive an injection of the gene is retained, compared to in vitro transduction of germ cells, because gene transfer is achieved without reducing the number of spermatogonial stem cells in the testis and transgenic sperms and transgenic animals can easily be prepared.

The invention discloses novel application of 12-HEPE or pharmaceutically acceptable fatty acid thereof, and comprises the application of the 12-HEPE or the pharmaceutically acceptable fatty acid thereof in preparation of a medicine for treating NOA. The 12-HEPE or the pharmaceutically acceptable fatty acid thereof can play a therapeutic role by promoting proliferation and differentiation of the endogenous spermatogonium of a mouse damaged by busulfan, and experiments prove that recovery of the thickness and the integrity of the spermatogenic epithelium of the testis and the recovery of the sperm concentration of the tail of epididymiscan be observed through intragastric administration of the 12-HEPE into the NOA mouse induced by the busulfan, meanwhile, proliferation and differentiation of spermatogonium are promoted, expression of the paracrine factors of cells is supported, so that the 12-HEPE can be used as a new target for alleviating the spermatogenesis dysfunction of the testis of the NOA mouse, and a new method is provided for treating the non-obstructive azoospermia.

The invention provides a Ddx5 gene deleted spermatogenesis disorder mouse model and a construction method thereof, and the inventor finds that a relationship exists between the Ddx5 gene and spermatogenic disorder or fertility dysfunction in research, the testicle volume of a male mouse with Ddx5 gene deletion is obviously reduced compared with that of a wild mouse. Moreover, the testicle of the mouse is free of germ cells, the epididymis is free of sperms, and any offspring cannot be obtained after the mouse is mated with a female mouse. Therefore, the Ddx5 gene deleted male mouse has the advantages of stability and thorough spermatogenic function inhibition, and is a good research model for male fertility disorders.

The invention provides application of geniposide in preparation of a medicine for treating asthenospermia. Experimental results show that the geniposide can significantly improve the sperm quantity and activity and reduce the sperm malformation rate, can significantly inhibit endoplasmic reticulum stress inside testis, increase mitochondrial membrane potential, regulate expression of related proteins in testis tissues, and improve excessive apoptosis of spermatogenic cells.

The invention discloses an application of bioactive polypeptide PACAP in preparation of drugs for improving fertility of obese men, and belongs to the field of damaged fertility diseases of the obesemen. The bioactive polypeptide PACAP drugs provided by the invention can significantly prevent and relieve obesity-induced male fertility damage (mainly by improving blood lipid and sex hormone levels, reducing a testicular tissue oxidative stress level, improving obesity-induced testicular spermatogenic cell damage, inhibiting high lipid-induced apoptosis of spermatogonia GC-1, and improving sperm function and early embryo quality after in-vitro fertilization), and repair pathological change and reproductive system function of fertility damage of the obese men, the relationship between obesity and male infertility can be initially understood, the protective effect of the PACAP on obesity-induced male reproductive damage can be clarified, and a theoretical basis, an experimental basis anda targeted treatment strategy are provided for clinical diagnosis and treatment of obese male infertility.

The invention provides a dissociation and separation method for shellfish spermary spermatogenic cells, and belongs to the technical field of the separation of ocean shellfish cells. The dissociationand separation method comprises the following steps of: the spermary of a shellfish during a proliferating phase is broken and is digested by a collagenase IV solution of which the mass concentrationis 0.1% and a trypsin solution of which the mass concentration is 0.25% in sequence, and spermary dissociation is carried out to obtain a single-cell suspension; and Percoll solutions of which the volume concentration is independently 10%, 22% and 35% is prepared, and the Percoll solutions are loaded in the same centrifuge tube in a descending order, the single-cell suspension of the shellfish spermatogenic cells is added into the top layer of the centrifuge tube, and centrifugation is carried out to independently collect three layers of cells. By use of a two-step enzymolysis method, spermatogenic cells in spermary tissues can be successfully dissociated into the single-cell suspension, a cell survival rate can be as high as 96.62%, and the cells can be normally subjected to in vitro normal culture. Meanwhile, high-purity spermatogonia and spermatocytes can be separated, an important material is provided for researching a shellfish gamete generating and breeding mechanism, and the dissociation and separation method has a high guidance meaning and a good application value.

The present invention provides a method of producing a non-human vertebrate that harbours germ cells having a desired gene transferred thereto, comprising injecting the desired gene to the testis of a non-human vertebrate wherein no tight junction exists between Sertoli cells to transfer the desired gene to germ cells, so as to obtain a non-human vertebrate that harbours the germ cells having the desired gene transferred thereto. Using the method of the present invention, even in animal species and lines for which in vitro transduction has been difficult to date, it is possible to obtain an individual harbouring germ cells, particularly spermatogonial stem cells, having a desired gene transferred thereto, at extremely high efficiency. Also, the fertility of the male to receive an injection of the gene is retained, compared to in vitro transduction of germ cells, because gene transfer is achieved without reducing the number of spermatogonial stem cells in the testis and transgenic sperms and transgenic animals can easily be prepared.

The invention belongs to the technical field of biology, relates to a detection method for detecting reproduction toxicity of BBP (Butyl Benzyl Phthalate), in particular relates to a detection method for detecting the reproduction toxicity of BBP by utilizing model organism zebra fishes and specifically relates to a method for rapidly detecting the male sperm production capability and female egg production capability of BBP by utilizing adult zebra fishes. According to the method disclosed by the invention, the experimental result displays that the detection period is only 8 days and is shorter than that of other methods reported in the existing literature; and moreover, the lowest concentration of the detected reproduction toxicity of BBP is 0.6mg.L<-1> and is superior to the detected concentration in other methods.

The invention relates to a pharmaceutical composition with a spermatogenic effect. The pharmaceutical composition is composed of effective components and medically acceptable auxiliary materials, andis characterized in that the effective components are a polysaccharide extract with a molecular weight of 3500 Daltons, wherein the polysaccharide extract is prepared from the following raw material medicines in percentage by weight: 37-55% of dried oyster meat, 23-35% of morinda roots, 14-22% of desertliving cistanche and 6-8% of apostichopus japonicus. The pharmaceutical composition disclosed bythe invention has the advantages of simple medicines, special effect and remarkable effect.

The invention discloses sperm piRNA (Piwi-interacting Ribonucleic Acid) marker combination related to male reproductive functional disturbance and application thereof. A marker comprises ten piRNAs (mainly comprising piR-1207 and piR-2107), and the sperm piRNA marker combination can be used for detection and prediction of sperm viability. In the invention, sperm can be relatively easily obtained, other tissues are not required, and the detection belongs to non-traumatic examination; sperm piRNA and MitoPLD (Phospholipase D) protein can be used for reflecting pathological and physiological status of a whole spermatogenic process at a molecular level, the accurate level of detection can be increased by taking the sperm piRNA and the MitoPLD protein as auxiliary detection indexes, and a potential target spot is provided for treatment of the male reproductive functional disturbance.

The invention belongs to the technical field of biomedicines. The invention particularly relates to application of canagliflozin and analogs thereof in preparation of a medicine for preventing and treating male reproductive dysfunction. Research results show that spermatogenic cells at all levels in seminiferous tubules of db / db mice treated by the canagliflozin reappear and are regularly arranged, meanwhile, a small amount of sperms exist in the tubules, and the canagliflozin treatment can be used for significantly improving abnormal sperm quality, abnormal sperm number and abnormal sperm movement of the db / db mice. The canagliflozin and analogs thereof are used for preventing or treating the male reproductive dysfunction for the first time, an ideal treatment effect is achieved, a new prevention and treatment choice is provided for clinical application and treatment of male sterility, especially male sterility caused by diabetes or hyperglycemia, and extremely high application values and social benefits are achieved.

The invention discloses application of a P substance in preparation of a drug for treating male infertility. Treatment of an azoospermia mouse model by the P substance finds that the P substance can effectively improve spermatogenic functions of the azoospermia mouse model caused by busulfan and further proves that the P substance improves the spermatogenic functions by promoting proliferation ofspermatogenic cells. The invention provides a new thought and theoretical support for treatment of male infertility.

The invention provides an antibody combination, a method and a detection kit for rapidly evaluating a testicular spermatogenic function. The antibody combination comprises (1) an FITC (fluorescein isothiocyanate) fluorescently-labeled rabbit antihuman GFRA1 antibody and a PE (phycoerythrin) fluorescently-labeled mouse antihuman c-KIT antibody, and (2) an FITC fluorescently-labeled rabbit antihuman SCP3 antibody and a PE fluorescently-labeled mouse antihuman PNA antibody. The method comprises the steps of taking a testiculus spermatogenic tubule as a detection sample, detecting expression conditions of GFRA1, c-KIT, SCP3 and PNA of the sample by immunohistochemical fluorescent staining, judging whether all spermatogenic cells exist, and then evaluating the spermatogenic function of a patient. According to the antibody combination, the method and the detection kit, immunofluorescent chemical staining of a germ cell surface marker is used for rapidly evaluating the spermatogenic function of the patient during / after an operation for the first time; the detection speed is high; the sensitivity is very high; the specificity is reliable; the spermatogenic cells in each stage can be distinguished; and a value for evaluation and subsequent treatment of the patient is important.

The invention discloses a radix morindae officinalis processed product oligosaccharide composition, and a preparation method and application thereof. The invention carries out separation on a radix morindae officinalis processed product to obtain a radix morindae officinalis processed product oligosaccharide composition.The oligosaccharide composition consists of Inulo type oligosaccharide and Inulin type oligosaccharide. According to the radix morindae officinalis processed product salt radix morindae officinalis oligosaccharide, the content of MDA can be remarkably reduced, and the activity of SOD, GSH-Px and CAT is enhanced to protect TM4 oxidative stress damage induced by H2O2. According to the radix morindae officinalis processed product radix morindae officinalis oligosaccharide, the content of MDA can be remarkably reduced, the activity of GSH-Px and CAT is enhanced, mouse germ cell oxidative damage caused by cyclophosphamide is protected, and reduction of the sperm malformation rate of male mice with spermatogenesis disorder caused by cyclophosphamide and improvement of sperm generation and sperm quality can be remarkably promoted. The radix morindae officinalis processed product oligosaccharide composition can be used for preparing health-care products or medicines for preventing or treating male reproductive system diseases.

The invention relates to a testicular tissue cryopreservation method based on single seminiferous tubule perfusion, which comprises the following steps: making testicular tissue into the single seminiferous tubule, soaking a single seminiferous tubule in a cryopreservation protective agent 1 for 10 minutes, and soaking and perfusing the seminiferous tubule by using a cryopreservation protective agent 2, so as to maximally reduce the permeation damage of the protective agent while achieve vitrification cooling, quickly transferring the poured spermatogenic tubule to a Cryo-genic single sperm cryopreservation slice, after sleeving of the Cryo-genic tubule, directly conducting immersing in liquid nitrogen to quickly achieve cooling, and quickly conducting soaking in a recovery solution aftercooling. Compared with a traditional testicular cell suspension cryopreservation method, the method disclosed by the invention has the advantages that (1) the complete structure of the seminiferous tubule is reserved; (2) the oxidative stress level of testicular cells is effectively reduced; (3) the death of a large number of spermatogonial cells and supportive cells after cryopreservation is effectively reduced; and (4) the recovery rate and activity of sperms in the spermatogenic tubules are high.

The invention discloses use of cyanidin-3-O-glucoside (C3G) and / or protocatechuic acid (PCA) in improving heat tolerance of testis. The cyanidin-3-O-glucoside and / or protocatechuic acid can relieve phenomena of atrophy of testis seminiferous tubules, vacuolation of spermatogenic epithelium, reduction of diameter and thickness of testis lumens and the like. The C3G and / or the metabolite PCA thereof can reduce a phosphorylation level of eIF2alpha and an expression of stress particles by reducing an external stress pressure suffered by the testis so as to increase heat tolerance of testis, improve an antioxidant system of the testis and regulate an IRE1alpha-XBP1 pathway so as to relieve oxidative stress and endoplasmic reticulum stress-mediated apoptosis and relieve spermatogenesis disorder and testis injury induced by heat stress. The C3G and / or the metabolite PCA thereof provides a reliable theoretical basis and hasimportant significance for relieving a male reproductive injury induced by the testis in a heat stress state due to bad living habits, occupational factors and pathological reasons in the future.

The invention provides an application of C3G in preparation of an antagonist for resisting oxidation system damage caused by 3-MCPD contamination. The invention proves that C3G improves the conditions of reduced feeding and slow growth of rats caused by 3-MCPD contamination; the C3G can effectively antagonize rat anti-oxidation system damage caused by 3-MCPD contamination and activate and regulate BTB structure functions of supporting cells by inhibiting an MAPK signal channel, so that spermatogenic epithelium damage caused by 3-MCPD is relieved, the stability of a spermatogenic microenvironment is maintained, division and differentiation of spermatogenic cells are regulated, and spermatogenic disorder is improved. Meanwhile, C3G can improve sperm activity by adjusting sperm energy metabolism related enzymes. Rat sex hormone secretion disorder caused by 3-MCPD contamination is regulated, and the normal level of sex hormone in serum and androgen in testis internal environment is maintained. The application of C3G in preparation of the antagonist for resisting oxidation system damage caused by the 3-MCPD contamination provides a theoretical basis for nutrition intervention of anthocyanin on 3-MCPD reproductive toxicity in the future.

The invention discloses an in-vitro separation method for primary spermatogenic cells of rats. The method comprises the following steps: 1) collecting the testis on two sides of a fresh in-vitro rat, and stripping envelopes and blood vessels to obtain testis tissues; 2) digesting the testicular tissue to prepare a single-cell suspension; 3) culturing the single-cell suspension until non-spermatogenic cells are adhered to the wall, sucking out non-adhered suspension cells, and separating the suspension cells by adopting a Percoll method to obtain the spermatogenic cells. According to the method, a foundation is laid for in-vitro research on the reproductive function of the rat testis, in-vitro separation and culture of the rat testis spermatogenic cells are achieved, and it is proved that the spermatogenic cells separated and cultured through the method are high in in-vitro survival rate and long in survival time. Normal rat testicular spermatogenic cells can be obtained by using the method, the cost is low, the operation is simple, the repeatability is high, various experimental requirements can be met, and the method is worthy of large-scale popularization.

The invention discloses a CCL8 protein serving as a biomarker of non-obstructive azoospermia and application of the CCL8 protein. The biomarker comprises a CCL8 protein. The invention finds that the concentration of the CCL8 protein in the seminal fluid of the NOA patient is obviously lower than that of a normal male for the first time, and proposes that the CCL8 protein can be used as a potential early warning molecule for evaluating the spermatogenic dysfunction of the NOA patient, so that expansion and diagnosis of NOA indexes are facilitated, the diagnosis range is expanded, and the CCL8 protein has important significance in the field of NOA diagnosis.

A traditional Chinese medicine preparation for treating male infertility, which is characterized in that it is composed of the following Chinese medicinal materials in the following weight ratio: 15-25 parts of Atractylodes Rhizome, 10-20 parts of Radix Glycyrrhizae, 10-20 parts of Poria cocos, and 25-35 parts of Cuscuta , 25‑35 parts of minced meat, 15‑25 parts of antler glue, 15‑25 parts of tortoise shell, 15‑25 parts of Polygonatum, 5‑9 parts of leech, 10‑20 parts of beehive dew, 15 parts of purple river car, 20‑20 parts of oyster 35 copies; the beneficial effects are: short course of treatment, no toxic and side effects, each course of treatment is one month, one course of treatment for mild patients, and three courses of treatment for severe patients can restore spermatogenic ability. The scope of application is: male infertility patients with kidney essence deficiency and essence cold infertility.

The invention relates to application of a c-Src / PI3K signal channel in reproductive injury protection, and relates to the technical field of biotechnology and medicine. The invention provides an application of a c-Src / PI3K signal channel as a target spot in preparation of drugs for treating reproductive injury, and provides a new action target spot for developing natural spermatogenic drugs.

The invention provides an antisense RNA sequence combination and method for inhibiting gonad development of pelteobagrus fulvidraco and application, and belongs to the technical field of molecular biology and breeding biology. The antisense RNA sequence combination for inhibiting gonad development of the pelteobagrus fulvidraco provided by the invention comprises antisense RNA for inhibiting an FSH receptor. The antisense RNA sequence combination can inhibit mRNA transcription of the FSH receptor of the pelteobagrus fulvidraco, thereby interfering with the generation and development of oocytesand spermatogenic cells, inhibiting gonad development of the pelteobagrus fulvidraco, and greatly shortening the marketing period of the pelteobagrus fulvidraco; and the antisense RNA sequence combination provided by the invention can also realize effective and accurate regulation and control of expression of a target gene, has extremely small damage to ova, is high in success rate and stable inbred offspring phenotype, and has an extremely good application prospect.

The invention belongs to the field of biochemical pharmacy and provides application of a swim bladder polypeptide to medicines for inhibiting oxidative damage and cell apoptosis in human bodies or animal bodies. The oxidative damage refer to testicle oxidative damage, the cell apoptosis refers to testicular spermatogenic cell apoptosis, the oxidative damage and the cell apoptosis are caused by antitumor drugs including busulfan, cyclophosphamide and thiotepa, and busulfan is used as the antitumor drug in an embodiment. The swim bladder polypeptide is capable of raising the expression level ofa transcription factor Nrf2, reducing an apoptosis promoting factor Bax and raising the expression level of an anti-apoptosis factor Bcl2; the swim bladder polypeptide is capable of relieving busulfaninduced germ cell vacuolization, spermatogenic cell apoptosis, seminiferous tubule degradation and sperm amount and viability reduction. By adoption of the swim bladder polypeptide for preparation ofthe medicines for inhibiting oxidative damage and cell apoptosis, medicines for recovering fertility of men or male animals can be prepared, and a direction is provided for treatment of fertility ofmen or male animals.

The invention relates to the field of animal models, in particular to a construction method and application of an azoospermia mouse model. According to the construction method of the azoospermia mouse model, a Pus7 gene on a mouse genome is knocked out. The important role of the Pus7 (Pseudouridine synthase 7) gene in the spermatogenesis process of mice is disclosed for the first time, male mice knocked out by the Pus7 have testicular dysplasia and azoospermia, and a mechanism of regulating testicular development by the Pus7 is expected to provide a new thought for research of male infertility mechanisms.