78 results about "Spermatogeny" patented technology

The invention relates to Chinese herbal medicine feed additive for stud rams, which mainly solves the problem that the sperm quality of stud rams is reduced due to centralized and high-intensity sperm collection in the prior art. The Chinese herbal medicine feed additive is prepared by crashing and mixing epimedium, actinolite, Chinese dodder seed, raspberry, Chinese magnoliavine fruit, astragalus root and dangshen and can be added into feed for usage. The invention improves ram sexual appetite, prompts spermatogenesis, increases sperm motility, effectively improves reproductive performance, solves the problem that the sperm quality of stud rams is reduced due to centralized and high-intensity sperm collection in the prior art according to spermatogenesis principle.

The invention discloses a method for predicting whether sperms exist in the testis of an azoospermia patient or not according to seminal plasma mRNA. The method comprises the following steps of dividing seminal fluid specimens of the azoospermia patient into a testicular sperm group and a testicular azoospermia group by taking a testicular biopsy as a gold standard, determining the difference of exRNA expression profiles of two seminal fluid groups, carrying out comparison with an existing spermatogenic cell transcriptome database, and searching for differentially expressed exRNA capable of judging spermatogenic cell types, which is used for judging whether haploid sperm cells exist in the testis of the azoospermia patient or not. The clinical application of the scheme provided by the invention can greatly improve the sperm taking success rate of the azoospermia patient, and is also helpful for guiding the azoospermia patient to select an economical, painless and effective treatment method to solve the problem of infertility of the azoospermia patient.

Applications of cyanidin-3-O-glucoside in preparation of medicines treating and/or preventing diseases caused by 3-chloropropane-1,2-diol are provided. The cyanidin-3-O-glucoside (C3G) can improve rat ingestion decrease and growth retardation which are caused by 3-MCPD poisoning, can effectively resist rat antioxidant system damage caused by 3-MCPD poisoning, and can activate and adjust BTB structural functions of sustentacular cells through inhibiting an MAPK signaling pathway, thus relieving seminiferous epithelium injuries caused by the 3-MCPD, maintaining stability of a spermatogenic microenvironment, regulating spermatogenic cell proliferation and differentiation, and improving dyszoospermia. In addition, the C3G can improve sperm activity through adjusting sperm energy metabolism related enzymes. The C3G can regulate rat sex hormone secretion disorders caused by 3-MCPD poisoning and can maintain normal levels of sex hormone in serum and androgen in testis inner environments. The applications provide theoretical foundations for 3-MCPD reproduction toxicity nutritional intervention utilizing anthocyanin.

The invention relates to an application of seabuckthorn fruit oil and/or whole fruit oil in preparing medicine promoting the formation of male sperms, preventing the apoptosis of spermatogenic cells or promoting the formation of female ovarian follicles and the maturation of germ cells and treating male and female sterility. By taking the preparation, kidney deficiency and infirmity and various chronic diseases can be recovered to the health status, more effectively, the invention has the functions of promoting the formation of the male sperms and protecting the quantity of the spermatogenic cells, and preventing the apoptosis of spermatogenic cells, and can obviously increase the quantity and the motor ability of the sperms, and increase the weight of spermary, and simultaneously has the functions of promoting the formation of the female ovarian follicles and the maturation of the germ cells, thereby being used as the medicine for the clinical treatment of male and female sterility. Compared with other medicines, the medicine has simple ingredients, belongs to target organ excitant, and has the reversible automatic regulation function of a second messenger, thereby being the medicine with high curative effect, security, and simplification.

The invention discloses novel application of 12-HEPE or pharmaceutically acceptable fatty acid thereof, and comprises the application of the 12-HEPE or the pharmaceutically acceptable fatty acid thereof in preparation of a medicine for treating NOA. The 12-HEPE or the pharmaceutically acceptable fatty acid thereof can play a therapeutic role by promoting proliferation and differentiation of the endogenous spermatogonium of a mouse damaged by busulfan, and experiments prove that recovery of the thickness and the integrity of the spermatogenic epithelium of the testis and the recovery of the sperm concentration of the tail of epididymiscan be observed through intragastric administration of the 12-HEPE into the NOA mouse induced by the busulfan, meanwhile, proliferation and differentiation of spermatogonium are promoted, expression of the paracrine factors of cells is supported, so that the 12-HEPE can be used as a new target for alleviating the spermatogenesis dysfunction of the testis of the NOA mouse, and a new method is provided for treating the non-obstructive azoospermia.

The invention provides a Ddx5 gene deleted spermatogenesis disorder mouse model and a construction method thereof, and the inventor finds that a relationship exists between the Ddx5 gene and spermatogenic disorder or fertility dysfunction in research, the testicle volume of a male mouse with Ddx5 gene deletion is obviously reduced compared with that of a wild mouse. Moreover, the testicle of the mouse is free of germ cells, the epididymis is free of sperms, and any offspring cannot be obtained after the mouse is mated with a female mouse. Therefore, the Ddx5 gene deleted male mouse has the advantages of stability and thorough spermatogenic function inhibition, and is a good research model for male fertility disorders.

The invention provides application of geniposide in preparation of a medicine for treating asthenospermia. Experimental results show that the geniposide can significantly improve the sperm quantity and activity and reduce the sperm malformation rate, can significantly inhibit endoplasmic reticulum stress inside testis, increase mitochondrial membrane potential, regulate expression of related proteins in testis tissues, and improve excessive apoptosis of spermatogenic cells.

The invention provides a dissociation and separation method for shellfish spermary spermatogenic cells, and belongs to the technical field of the separation of ocean shellfish cells. The dissociationand separation method comprises the following steps of: the spermary of a shellfish during a proliferating phase is broken and is digested by a collagenase IV solution of which the mass concentrationis 0.1% and a trypsin solution of which the mass concentration is 0.25% in sequence, and spermary dissociation is carried out to obtain a single-cell suspension; and Percoll solutions of which the volume concentration is independently 10%, 22% and 35% is prepared, and the Percoll solutions are loaded in the same centrifuge tube in a descending order, the single-cell suspension of the shellfish spermatogenic cells is added into the top layer of the centrifuge tube, and centrifugation is carried out to independently collect three layers of cells. By use of a two-step enzymolysis method, spermatogenic cells in spermary tissues can be successfully dissociated into the single-cell suspension, a cell survival rate can be as high as 96.62%, and the cells can be normally subjected to in vitro normal culture. Meanwhile, high-purity spermatogonia and spermatocytes can be separated, an important material is provided for researching a shellfish gamete generating and breeding mechanism, and the dissociation and separation method has a high guidance meaning and a good application value.

The invention belongs to the technical field of biology, relates to a detection method for detecting reproduction toxicity of BBP (Butyl Benzyl Phthalate), in particular relates to a detection method for detecting the reproduction toxicity of BBP by utilizing model organism zebra fishes and specifically relates to a method for rapidly detecting the male sperm production capability and female egg production capability of BBP by utilizing adult zebra fishes. According to the method disclosed by the invention, the experimental result displays that the detection period is only 8 days and is shorter than that of other methods reported in the existing literature; and moreover, the lowest concentration of the detected reproduction toxicity of BBP is 0.6mg.L<-1> and is superior to the detected concentration in other methods.

The invention discloses application of a P substance in preparation of a drug for treating male infertility. Treatment of an azoospermia mouse model by the P substance finds that the P substance can effectively improve spermatogenic functions of the azoospermia mouse model caused by busulfan and further proves that the P substance improves the spermatogenic functions by promoting proliferation ofspermatogenic cells. The invention provides a new thought and theoretical support for treatment of male infertility.

The invention provides an antibody combination, a method and a detection kit for rapidly evaluating a testicular spermatogenic function. The antibody combination comprises (1) an FITC (fluorescein isothiocyanate) fluorescently-labeled rabbit antihuman GFRA1 antibody and a PE (phycoerythrin) fluorescently-labeled mouse antihuman c-KIT antibody, and (2) an FITC fluorescently-labeled rabbit antihuman SCP3 antibody and a PE fluorescently-labeled mouse antihuman PNA antibody. The method comprises the steps of taking a testiculus spermatogenic tubule as a detection sample, detecting expression conditions of GFRA1, c-KIT, SCP3 and PNA of the sample by immunohistochemical fluorescent staining, judging whether all spermatogenic cells exist, and then evaluating the spermatogenic function of a patient. According to the antibody combination, the method and the detection kit, immunofluorescent chemical staining of a germ cell surface marker is used for rapidly evaluating the spermatogenic function of the patient during/after an operation for the first time; the detection speed is high; the sensitivity is very high; the specificity is reliable; the spermatogenic cells in each stage can be distinguished; and a value for evaluation and subsequent treatment of the patient is important.

The invention relates to a testicular tissue cryopreservation method based on single seminiferous tubule perfusion, which comprises the following steps: making testicular tissue into the single seminiferous tubule, soaking a single seminiferous tubule in a cryopreservation protective agent 1 for 10 minutes, and soaking and perfusing the seminiferous tubule by using a cryopreservation protective agent 2, so as to maximally reduce the permeation damage of the protective agent while achieve vitrification cooling, quickly transferring the poured spermatogenic tubule to a Cryo-genic single sperm cryopreservation slice, after sleeving of the Cryo-genic tubule, directly conducting immersing in liquid nitrogen to quickly achieve cooling, and quickly conducting soaking in a recovery solution aftercooling. Compared with a traditional testicular cell suspension cryopreservation method, the method disclosed by the invention has the advantages that (1) the complete structure of the seminiferous tubule is reserved; (2) the oxidative stress level of testicular cells is effectively reduced; (3) the death of a large number of spermatogonial cells and supportive cells after cryopreservation is effectively reduced; and (4) the recovery rate and activity of sperms in the spermatogenic tubules are high.

The invention discloses use of cyanidin-3-O-glucoside (C3G) and/or protocatechuic acid (PCA) in improving heat tolerance of testis. The cyanidin-3-O-glucoside and/or protocatechuic acid can relieve phenomena of atrophy of testis seminiferous tubules, vacuolation of spermatogenic epithelium, reduction of diameter and thickness of testis lumens and the like. The C3G and/or the metabolite PCA thereof can reduce a phosphorylation level of eIF2alpha and an expression of stress particles by reducing an external stress pressure suffered by the testis so as to increase heat tolerance of testis, improve an antioxidant system of the testis and regulate an IRE1alpha-XBP1 pathway so as to relieve oxidative stress and endoplasmic reticulum stress-mediated apoptosis and relieve spermatogenesis disorder and testis injury induced by heat stress. The C3G and/or the metabolite PCA thereof provides a reliable theoretical basis and hasimportant significance for relieving a male reproductive injury induced by the testis in a heat stress state due to bad living habits, occupational factors and pathological reasons in the future.

The invention discloses an in-vitro separation method for primary spermatogenic cells of rats. The method comprises the following steps: 1) collecting the testis on two sides of a fresh in-vitro rat, and stripping envelopes and blood vessels to obtain testis tissues; 2) digesting the testicular tissue to prepare a single-cell suspension; 3) culturing the single-cell suspension until non-spermatogenic cells are adhered to the wall, sucking out non-adhered suspension cells, and separating the suspension cells by adopting a Percoll method to obtain the spermatogenic cells. According to the method, a foundation is laid for in-vitro research on the reproductive function of the rat testis, in-vitro separation and culture of the rat testis spermatogenic cells are achieved, and it is proved that the spermatogenic cells separated and cultured through the method are high in in-vitro survival rate and long in survival time. Normal rat testicular spermatogenic cells can be obtained by using the method, the cost is low, the operation is simple, the repeatability is high, various experimental requirements can be met, and the method is worthy of large-scale popularization.

The invention discloses a CCL8 protein serving as a biomarker of non-obstructive azoospermia and application of the CCL8 protein. The biomarker comprises a CCL8 protein. The invention finds that the concentration of the CCL8 protein in the seminal fluid of the NOA patient is obviously lower than that of a normal male for the first time, and proposes that the CCL8 protein can be used as a potential early warning molecule for evaluating the spermatogenic dysfunction of the NOA patient, so that expansion and diagnosis of NOA indexes are facilitated, the diagnosis range is expanded, and the CCL8 protein has important significance in the field of NOA diagnosis.

The invention relates to application of a c-Src/PI3K signal channel in reproductive injury protection, and relates to the technical field of biotechnology and medicine. The invention provides an application of a c-Src/PI3K signal channel as a target spot in preparation of drugs for treating reproductive injury, and provides a new action target spot for developing natural spermatogenic drugs.

The invention provides an antisense RNA sequence combination and method for inhibiting gonad development of pelteobagrus fulvidraco and application, and belongs to the technical field of molecular biology and breeding biology. The antisense RNA sequence combination for inhibiting gonad development of the pelteobagrus fulvidraco provided by the invention comprises antisense RNA for inhibiting an FSH receptor. The antisense RNA sequence combination can inhibit mRNA transcription of the FSH receptor of the pelteobagrus fulvidraco, thereby interfering with the generation and development of oocytesand spermatogenic cells, inhibiting gonad development of the pelteobagrus fulvidraco, and greatly shortening the marketing period of the pelteobagrus fulvidraco; and the antisense RNA sequence combination provided by the invention can also realize effective and accurate regulation and control of expression of a target gene, has extremely small damage to ova, is high in success rate and stable inbred offspring phenotype, and has an extremely good application prospect.

The invention relates to the field of animal models, in particular to a construction method and application of an azoospermia mouse model. According to the construction method of the azoospermia mouse model, a Pus7 gene on a mouse genome is knocked out. The important role of the Pus7 (Pseudouridine synthase 7) gene in the spermatogenesis process of mice is disclosed for the first time, male mice knocked out by the Pus7 have testicular dysplasia and azoospermia, and a mechanism of regulating testicular development by the Pus7 is expected to provide a new thought for research of male infertility mechanisms.