Oxygen self-supply type targeted nano photodynamic therapy system

A therapeutic system, nano-light technology, used in the field of biomedicine to solve problems such as limitations

Active Publication Date: 2019-09-06
SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the inherent hypoxic state of tumor tissue and the consumption of molecular oxygen in the tumor microenvironment by photodynamic therapy greatly limit the efficacy of highly oxygen-dependent PDT and become an important obstacle to the clinical application of PDT.

Method used

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  • Oxygen self-supply type targeted nano photodynamic therapy system
  • Oxygen self-supply type targeted nano photodynamic therapy system
  • Oxygen self-supply type targeted nano photodynamic therapy system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Construction and Characterization of Embodiment 1 Nanoparticles

[0054] 1 Reagents and instruments

[0055] 1.1 Instrument

[0056] BT224S electronic balance (Sartorius company); JY92-Ⅱ ultrasonic cell pulverizer (Ningbo Xinzhi Biotechnology Co., Ltd.); R-200 rotary evaporator (Buchi company); 81-2 constant temperature magnetic stirrer (Shanghai Sile Instrument Factory); AG 5810R low-temperature high-speed centrifuge (Eppendorf Company); Malvern Zetasizer Nano ZS laser particle size analyzer (Malvern Company); CM-120 transmission electron microscope (Philips Company); ultrapure water system (Millipore Company); Bed (Eppendorf); cell culture incubator (Thermo); DP50 upright microscope (Olympus); 1300 Series ultra-clean bench (Thermo Scientific); multi-functional microplate reader (Thermo Scientific); ice machine (IMI Cornelius Company); Leica TCS SP8 laser confocal microscope (Leica Company); HSS constant temperature water bath (Chongqing Star Company); inductively co...

Embodiment 2

[0096] Example 2 Pt@HLipo nanoparticles enhance photodynamic therapy by alleviating intratumoral hypoxia

[0097] 1 Instruments and materials

[0098] 1.1 Main Instruments

[0099] Tissue oxygen partial pressure monitoring system (UK / Oxford Optronix); BT224S electronic balance (Sartorius company); JY92-Ⅱ ultrasonic cell pulverizer (Ningbo Xinzhi Biotechnology Co., Ltd.); R-200 rotary evaporator (Buchi company) ; 81-2 type constant temperature magnetic stirrer (Shanghai Sile Instrument Factory); AG 5810R low temperature high-speed centrifuge (Eppendorf company); Malvern ZetasizerNano ZS laser particle size analyzer (Malvern company); CM-120 transmission electron microscope (Philips company); Pure water system (Millipore); Innova 40 desktop constant temperature shaker (Eppendorf); cell culture incubator (Thermo); DP50 upright microscope (Olympus); 1300 Series ultra-clean bench (Thermo Scientific); Instrument (Thermo Scientific Company); ice machine (IMI Cornelius Company); Lei...

Embodiment 3

[0123] Example 3 Study on the ability of Pt@HLipo to target tumor blood vessels in vivo

[0124] 1 Instruments and materials

[0125] 1.1 Main Instruments

[0126] BT224S electronic balance (Sartorius company); slide (ibidiμ-slide I 0.4 ); AG 5810R low-temperature high-speed centrifuge (Eppendorf); ultrapure water system (Millipore); cell culture incubator (Thermo); DP50 upright microscope (Olympus); 1300Series ultra-clean bench (Thermo Scientific); Functional microplate reader (Thermo Scientific Company); ice maker (IMI Cornelius Company); Leica TCS SP8 laser confocal microscope (Leica Company); HSS constant temperature water bath (Chongqing Star Company); peristaltic pump (Baoding Chuangrui Pump Industry Co., Ltd. company); IVISSpectrum-CT imaging system (PerkinElmer); HVE-50 autoclave (Hirayama Corporation).

[0127] 1.2 Materials and reagents

[0128] Suture needle with thread (Shanghai Pudong Jinhuan Medical Supplies Co., Ltd.); DPBS buffer solution (Yuanpei Biotechnolo...

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Abstract

The invention relates to an oxygen self-supply type targeted nano photodynamic therapy system. The oxygen self-supply type targeted nano photodynamic therapy system comprises a liposome loaded with Ptnanoparticles and a photosensitizer verteporfin simultaneously and a macrophage membrane, wherein the liposome and the macrophage membrane are hybridized to form a hybrid membrane. The invention alsorelates to a preparation method and application of the oxygen self-supply type targeted nano photodynamic therapy system, the liposome nanoparticles loaded with the Pt nanoparticles and the photosensitizing agent verteporfin at the same time are successfully synthesized through an improved reverse evaporation method, hybridization of the liposome and the macrophage membrane is realized through afreezing and thawing method, and the characteristics of tumor targeting of the liposome are endowed. The obtained nano liposome is regular in shape, uniform in particle size and high in encapsulationefficiency, and photodynamic therapy and chemotherapy are organically combined together, so that an effect of inhibiting tumor cells in combination with photochemotherapy is achieved.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to an oxygen self-sufficient targeted nanometer photodynamic therapy system. Background technique [0002] Malignant tumors are still a major disease that seriously threatens human health. In recent years, the number of new cancer cases and deaths worldwide has increased alarmingly. Traditional tumor treatment methods, such as radiotherapy and chemotherapy, are not very effective in treating cancer. With the rapid development of nanotechnology, nanomedicine provides broad prospects for the treatment of tumors. Nanotechnology can improve the pharmacokinetics and pharmacodynamics of traditional drugs, thereby improving the therapeutic effect of existing drugs. With the intersection of various disciplines, nanomedicine technology is also developing in a more diverse and comprehensive direction. [0003] Photodynamic therapy (PDT) is a new type of effective method for treating tu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K9/127A61K47/46A61K33/243A61P35/00
CPCA61K9/127A61K9/1277A61K41/0071A61K47/46A61P35/00A61K33/243A61K2300/00
Inventor 方超刘学良陆琴陈红专
Owner SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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