Preparation methods of natural biomass quantum dot and biomass quantum dot-copper nanocomposite and application of natural biomass quantum dot

Abstract

The invention relates to preparation methods of a natural biomass quantum dot and a biomass quantum dot-copper nanocomposite and application of the natural biomass quantum dot. The preparation methodof the biomass quantum dot comprises the following steps: S1, grinding spinach leaves into paste; S2, adding a mixed solution of ethanol and acetone, uniformly mixing and filtering; S3, adding oleic acid, polyethylene glycol diamine and a magnetic stir bar into a three-neck flask, pumping air out after sealing, introducing argon, stirring, heating to 170 to 190 DEG C, and stopping heating when thesolution is in orange-yellow color; S4, cooling the solution to room temperature, adding a chlorophyll crude extracting solution, heating the solution to 170 to 190 DEG C under the protection of theargon and magnetic stirring, and keeping the temperature for 170 to 190 minutes; S5, naturally cooling the solution to room temperature, and adding concentrated HCl till the solution is in strong acidity; S6, standing for layering, and collecting subnatant; S7, adjusting the pH value of the solution to neutral with a saturated NaOH solution, filtering larger particles, and performing dialysis to obtain a biomass quantum dot solution. The biomass quantum dot solution has the characteristics of high fluorescence intensity, high light stability and high biocompatibility.

Description

technical field [0001] The present invention relates to the technical field of biological cell detection, in particular to the detection of cell lesions and photodynamic therapy agent technology, specifically to the preparation method and application of natural biomass quantum dots and biomass quantum dot-copper nanocomposites. Background technique [0002] Photodynamic therapy (PDT) is a new laser medical technology for the treatment of local lesions in the human body, especially malignant tumors. It utilizes the selective aggregation characteristics of photosensitizers in the human body. When a series of photochemical reactions occur, reactive oxygen species (ROS) are generated to kill tumors or other pathological tissues and achieve the purpose of treatment. However, in the process of tumor treatment, the ROS generated by photosensitizers must first face the resistance of the overexpressed reducing biothiol compounds in cancer cells, which makes the PDT effect significant...

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to solve the technical problems in the prior art that the imaging of biological thiol compounds in vivo and efficient photodynamic therapy of tumors cannot be performed simultaneously, and provides a rich Preparation method of near-infrared natural biological quantum dots of chlorophyll and preparation method and application of biological quantum dots-copper nanocomposites

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