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Sonosensitizer with oxygen-carrying function

A sound sensitizer and functional technology, applied in the field of biomedicine, to achieve the effect of enhancing the effect of sound dynamics

Inactive Publication Date: 2008-02-20
许川山
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Decreased tissue oxygen limits sonosensitivity and affects the killing effect of SDT

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1: Preparation of hematoporphyrin-loaded and perfluorocarbon microbubble compositions:

[0016] The sound sensitizer is hematoporphyrin 2 mg, film-forming material distearyl phosphatidylcholine 5 mg, dipalmitoyl phosphatidylethanolamine 2 mg, phosphatidic acid 1 mg, poloxamer 0.5 mg, glycerol 50 μl and phosphate buffer 450 μl Put the small cylindrical tube in a water bath at 37°C for 30 minutes, cool to room temperature, fill it with perfluorocarbon as an oxygen-carrying substance, and shake it mechanically for 50 seconds to obtain a hematoporphyrin-loaded and perfluorocarbon microbubble composition at -4°C Or store at -20°C airtight and avoid sound.

Embodiment 2

[0017] Example 2: Preparation of albumin microbubble composition loaded with piroxicam and perfluorocarbon

[0018] Mix 20% human serum albumin and 5% glucose in a ratio of 1:5, then vibrate for 80s with a vibrometer at 30% of the maximum output power, and at the same time slowly fill in 0.6ml of perfluorocarbon material through the three-way tube , adding piroxicam according to the mass / volume ratio (4:1), fully shaking or mixing, and freeze-drying to prepare the albumin microbubble composition loaded with piroxicam and perfluorocarbon.

Embodiment 3

[0019] Embodiment 3: Determination of encapsulation efficiency of hematoporphyrin and perfluorocarbon composition

[0020] Take the hematoporphyrin and perfluorocarbon composition prepared in Example 1, centrifuge at a speed of 500 rpm for 5 minutes, take the upper layer of microbubbles, fully dissolve composition 1 with 800 μl dimethyl sulfoxide and 3200 μl absolute alcohol Finally, dilute it with phosphate buffer to an appropriate multiple, and then measure its sound absorption value with a fluorescent sound spectrometer, using phosphate buffer as a reference solution. The measured drug encapsulation rate reaches 25%-57%.

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PUM

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Abstract

The present invention pertains to the biomedical field, more particularly, the present invention relates to a sound-sensitive agent which has the oxygen carrying function. The sound-sensitive agent is a composition which is composed of the substances with the oxygen carrying function and the sound-sensitive substances. The present invention makes use of the substances with the oxygen carrying function to improve the oxygen content of the target area, strengthen the sonodynamic effect of the sound-sensitive substances and develop the safe and effective sound-sensitive agent. The sound-sensitive agent which has the oxygen carrying function can be used as the sound-sensitive agent during the sonodynamic treatment process and can be used as the synergistic agent during the low-intensity / high-intensity focused / non-focused ultrasound treatment process, so the present invention is applicable to the skin care, obesity, benign and malignant proliferative diseases.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a sound sensitizer with an oxygen-carrying function comprising an oxygen-carrying functional substance. Background technique [0002] Sonodynamic therapy (SDT) is a new method for the treatment of malignant tumors that has emerged with the continuous development of photodynamic therapy. Under the acoustic excitation of the wavelength, a series of sonochemical reactions are caused to cause irreversible damage to tumor cells to achieve the purpose of cancer treatment. Ultrasound, as a mechanical wave, has strong penetrating power to biological tissues, and focused ultrasound can concentrate sound energy in deep tissues without trauma. Sonodynamic therapy is expected to develop into a promising new method for tumor treatment. [0003] During the practice of photodynamic therapy, people found that photodynamic therapy is to achieve photodynamic reaction on the target tissue by...

Claims

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Application Information

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IPC IPC(8): A61K45/00A61P35/00
Inventor 许川山张勇王志刚
Owner 许川山
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