A kind of recombinant cell and its application in synthesizing α, β-unsaturated aldehydes
A technology for recombining cells and recombining carriers, which is applied in the field of α,β-unsaturated aldehyde synthesis to achieve the effects of improving catalytic efficiency, high selectivity and shortening reaction time
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Embodiment 1
[0038] Example 1 Acquisition of Enol Dehydrogenase Encoding Gene of Yorkella sp.WZY002
[0039] Using the published enol dehydrogenase (YsADH) encoding gene (GenBank accession number KF887947) from Yorkella sp. WZY002, after codon optimization, artificial synthesis (Suzhou Jinweizhi Biotechnology Co., Ltd. Provide gene synthesis service) The gene encoding enol dehydrogenase, the amino acid sequence and nucleotide sequence are respectively shown in SEQ ID NO.1 and SEQ ID NO.2.
Embodiment 2
[0040] Example 2 Acquisition of gene encoding NADPH oxidase from Thermococcus kodakaraensis
[0041] Using the published gene encoding NADPH oxidase (TkNOX) from Thermococcus kodakaraensis (GenBank accession number is BAD85488), after codon optimization, artificially synthesized (Gene synthesis service provided by Hangzhou Qingke Biotechnology Co., Ltd.) The NADPH oxidase (TkNOX) coding gene, the amino acid sequence and nucleotide sequence are respectively shown in SEQ ID NO.3 and SEQ ID NO.4.
Embodiment 3
[0042] Example 3 Acquisition of the gene encoding hemoglobin from Vitreoscilla stercoraria
[0043]Using the published gene encoding hemoglobin (VsHGB) from Vitreoscilla stercoraria (GenBank accession number is AAA27584), after codon optimization, artificially synthesized (Gene synthesis service provided by Hangzhou Qingke Biotechnology Co., Ltd.) Myoglobinase (VsHGB) encoding gene, the amino acid sequence and nucleotide sequence are respectively shown in SEQ ID NO.5 and SEQ ID NO.6.
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