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Novel photosensitive agent with function of carrying oxygen

A photosensitizer and functional technology, applied in the field of biomedicine, to achieve the effect of enhancing the effect of photodynamic effect

Inactive Publication Date: 2008-02-27
许川山
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Decreased tissue oxygen limits photosensitivity and affects the killing effect of PDT

Method used

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  • Novel photosensitive agent with function of carrying oxygen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: Preparation of hematoporphyrin-loaded and perfluorocarbon microbubble compositions:

[0017] The photosensitizer uses hematoporphyrin 2mg, film-forming material distearyl phosphatidylcholine 5mg, dipalmitoylphosphatidylethanolamine 2mg, phosphatidic acid 1mg, poloxamer 0.5mg, glycerol 50μl and phosphate buffer 450μl and put them into a cylinder In a small tube, put it in a water bath at 37°C for 30 minutes, after cooling to room temperature, fill it with oxygen-carrying substance perfluorocarbon, and mechanically shake it for 50s to obtain hematoporphyrin and perfluorocarbon microbubble composition, -4°C Or store at -20°C airtight and protected from light. See Figure 1: uniformly dispersed hematoporphyrin and perfluorocarbon microbubbles can be seen in the figure.

Embodiment 2

[0018] Example 2: Preparation of Albumin Microbubble Composition Loaded with Hypocretin B and Perfluorocarbon

[0019] Mix 20% human serum albumin and 5% glucose at a ratio of 1:5, then vibrate for 80s with a vibrometer at 30% of the maximum output power, and at the same time slowly fill in 0.6ml of perfluorocarbon material through the three-way tube , adding hypocrellin B according to the mass / volume ratio (4:1), fully shaking or mixing, and freeze-drying to prepare the albumin microbubble composition loaded with hypocrellin B and perfluorocarbon.

Embodiment 3

[0020]Embodiment 3: Determination of encapsulation efficiency of hematoporphyrin and perfluorocarbon composition

[0021] Take the hematoporphyrin and perfluorocarbon composition prepared in Example 1, centrifuge at a speed of 500 rpm for 5 minutes, take the upper layer of microbubbles, fully dissolve composition 1 with 800 μl dimethyl sulfoxide and 3200 μl absolute alcohol Finally, it was diluted with phosphate buffer solution to an appropriate multiple, and then the absorbance was measured with a fluorescence spectrophotometer, using phosphate buffer solution as a reference solution. The measured drug encapsulation rate reaches 25%-57%.

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Abstract

The invention discloses a new typed photosensitizer with oxygen-carrying function in the biological medical domain, which is the composition of oxygen-carrying material and photosensitizer to improve oxygen content in the target area and reinforce the optical dynamic effect of photosensitizer. The invention can be photosensitizer in the optical dynamic treating course and booster in the low strength / high strength focusing / non-focusing hypersonic treating course, which is fit for skin beauty, obesity and benign, malignant proliferative disease.

Description

technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to a novel photosensitizer with an oxygen-carrying function comprising an oxygen-carrying functional substance. Background technique [0002] Photodynamic therapy (PDT) is a new method for the treatment of malignant tumors that has emerged with the development of fiber optic technology, laser medicine and endoscopic technology. Its basic principle is that malignant tumors can selectively absorb and retain photosensitizers , under the light excitation of a specific wavelength, a series of photochemical reactions are caused to cause irreversible damage to tumor cells to achieve the purpose of cancer treatment. Since Dougherty et al. reported photodynamic therapy for the first time, photodynamic therapy has been carried out in many tumor treatments. Photodynamic therapy makes full use of the dual selection effect of selective laser irradiation on lesions and selective accumulatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61P35/00A61P3/04
Inventor 许川山张勇王志刚
Owner 许川山
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