Preparation method of biological mimetic tissue adhesive

A tissue adhesive and bioadhesive technology, applied in the field of medical biomaterials, can solve the problems of cumbersome preparation process and poor biocompatibility, and achieve the effect of simple purification process, good adhesion performance and mild reaction conditions

A tissue adhesive and bioadhesive technology, applied in the field of medical biomaterials, can solve the problems of cumbersome preparation process and poor biocompatibility, and achieve the effect of simple purification process, good adhesion performance and mild reaction conditions

CN106390185AInactive Publication Date: 2017-02-15SHANGHAI QISHENG BIOLOGICAL PREPARATION CO LTD
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  • Preparation method of biological mimetic tissue adhesive
  • Preparation method of biological mimetic tissue adhesive
  • Preparation method of biological mimetic tissue adhesive

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Dissolve 0.5g of sodium hyaluronate dry powder (molecular weight: 2,000,000DA) in 25mL of water, adjust the pH value to 5, then add 0.25g of dopamine hydrochloride and 0.5g of DMTMM, fill with nitrogen to deoxygenate and stir for 24 hours in the dark. . Weigh 0.034g of polylysine, and add 0.0986g of DMTMM into the above mixed system, and react for 6h under the condition of protecting from light by filling with nitrogen gas for deoxygenation. Dialyzed against PBS buffer solution with pH=5 for 2 days (molecular weight cut-off 10000), then dialyzed with PBS buffer solution with pH=7.2 for 2 days (molecular weight cut-off 10000), freeze-dried to obtain sample A, ready for use.

[0028] Dissolve 0.1 g of sample A in 5 mL of PBS solution. After the dissolution is complete, add 50 uL of 3% sodium periodate solution, mix quickly, and obtain a bioadhesive at room temperature.

Embodiment 2

[0030] Dissolve 0.5g of sodium hyaluronate dry powder (molecular weight: 310,000DA) in 25mL of water, adjust the pH to 5, then add 0.5g of dopamine hydrochloride and 0.5g of DMTMM, fill with nitrogen to deoxygenate and stir for 48 hours in the dark. . Weigh 0.034g of polylysine, and add 0.0986g of DMTMM into the above mixed system, and react for 6h under the condition of protecting from light by filling with nitrogen gas for deoxygenation. Dialyzed against PBS buffer with pH=5 for 2 days (molecular weight cut-off 10000), then dialyzed against PBS buffer with pH=7.2 for 2 days (molecular weight cut-off 10000), freeze-dried to obtain sample A, ready for use.

[0031] Dissolve 0.1 g of sample A in 1 mL of PBS solution. After the dissolution is complete, add 60 uL of 5% sodium periodate solution, mix quickly, and obtain a bioadhesive at room temperature.

Embodiment 3

[0033]Dissolve 0.6g of sodium hyaluronate dry powder (molecular weight: 710,000DA) in 25mL of water, adjust the pH to 5, then add 0.5g of dopamine hydrochloride and 1g of DMTMM, inflate with nitrogen to deoxygenate and stir for 24 hours in the dark. Weigh 0.034g of polylysine, and add 0.0986g of DMTMM into the above mixed system, and react for 6h under the condition of protecting from light by filling with nitrogen gas for deoxygenation. Dialyzed against PBS buffer with pH=5 for 2 days (molecular weight cut-off 10000), then dialyzed with PBS buffer with pH=7.2 for 2 days (molecular weight cut-off 10000), freeze-dried to obtain sample A, ready for use.

[0034] Dissolve 0.1 g of sample A in 2.5 mL of PBS solution. After the dissolution is complete, add 80 uL of 3% sodium periodate solution, mix quickly, and obtain a bioadhesive at room temperature.

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Abstract

The invention relates to a preparation method of a biological mimetic and structural bionic tissue adhesive in order to overcome disadvantages of present technological technologies for preparing mussel mucoprotein bionic biological tissue adhesives and improve the viscosity, the biocompatibility and the structural bionic property of a material. The preparation method comprises the following steps: grafting a natural polymer material with dopamine or other catechol group-containing derivatives through an amidation reaction by adopting DMTMM as a carboxyl group activator; and further modifying the grafted natural polymer material with lysine or polylysine by adopting the same technology to obtain the novel biological mimetic and structural bionic tissue adhesive containing dopamine analog and polylysine analog. The tissue adhesive plays a full role in tissue adhesion in wet and physiologic environment, has good tissue adherence and high biological safety, and can be slowly degraded in the tissue healing process until complete degradation. The preparation method of the biological adhesive has the advantages of simplicity, mild reaction conditions, increase of the reaction efficiency, improvement of the operability of the enterprise production process, and facilitation of amplified production of enterprises.

Description

technical field [0001] The invention relates to the technical field of medical biomaterials, in particular to a biomimetic adhesive and a preparation method thereof. It has potential application value in the bonding of traumatic skin tears and surgery-free suturing. Background technique [0002] Over the years, due to the advantages of strong sutures and difficult wound dehiscence, suturing wounds with sutures, rivets and other materials or instruments is the most commonly used and most effective wound bonding method in clinical practice. However, with the rapid development of modern medicine, the clinical requirements for surgical methods and auxiliary materials are getting higher and higher. In order to reduce the complexity of surgery and trauma to patients, medical adhesives have been developed rapidly and widely used. . [0003] Medical adhesives refer to preparations, materials or substances that can be adhered to surfaces or cause adhesion between surfaces in medica...

Claims

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Application Information

Patent Timeline
15 Feb 2017
Publication
CN106390185A
IPC
A61L24/08; A61L24/00
CPC
A61L24/08; A61L24/001; A61L24/0031; C08L5/08
Inventors
魏长征; 钱锵