54results about How to "Easy to operate and promote" patented technology

The invention relates to a pH and oxidation-reduction dual-sensitive layer cross-linking nanoparticle as well as a preparation method and an application thereof. A hydrophilic layer which at least contains PCB (Polycarboxylate Betaine) or PEG (Polyethylene Glycol) is positioned on the surface of the nanoparticle, a hydrophobic nuclei which at least contains a pH-sensitive polymer unit is positioned in the nanoparticle, and an S-S (Disulfide Bond) cross-linking layer which is formed through cross-linking reaction between PDS (Polymethacrylamide Ehtylpyridine Disulfide) units is positioned between the hydrophilic layer and the hydrophobic nuclei. A tumor targeted group can be modified on the hydrophilic layer positioned on the surface of the pH and oxidation-reduction dual-sensitive layer cross-linking nanoparticle, and the gathering of the nanoparticle on a tumor tissue and the endocytosis of the nanoparticle on the tumor cell are accelerated through the specific targeted effect of the nanoparticle on a tumor cell. The pH and oxidation-reduction dual-sensitive layer cross-linking nanoparticle disclosed by the invention has the advantages of good biocompatibility and low toxicity. According to the pH and oxidation-reduction dual-sensitive layer cross-linking nanoparticle, a cross-linked structure is dissociated under the action of glutathione inside the tumor cell, so that the release of a medicine is promoted. The preparation method disclosed by the invention is simple, convenient, good in stability and conveniently operated and popularized.

The invention discloses a rich media direct broadcasting business system and method. The system comprises a direct broadcasting client, a direct broadcasting management server and a media distribution system, wherein the direct broadcasting client is used for applying for a media distribution service capability from the direct broadcasting management server to obtain rich media direct broadcasting channel addresses, obtaining rich media resources in real time and transmitting the rich media resources to the media distribution system in a rich media data stream manner; the direct broadcasting management server is used for distributing the media distribution service capability and the rich media direct broadcasting channel addresses to the direct broadcasting client; and the media distribution system is used for receiving the rich media data stream from the direct broadcasting client, and transmitting the corresponding rich media data stream to a rich media player according to the requested rich media direct broadcasting channel address. By utilizing the rich media direct broadcasting business system and method disclosed by the invention, the media resources obtained by terminals, such as a mobile terminal/PC (Personal Computer)/STB (Set Top Box) and the like, can be directly broadcasted on the internet so that users can conveniently watch direct broadcasting content from a direct broadcaster anytime and anywhere.

The invention relates to a nanoparticle and a preparation method thereof. Poly(lactide-glycolide acid) (PLGA) is in the center to serve as a core, phospholipid surrounds the surface of the PLGA core in monolayer and distearoyl phosphatidylethanolamine-polyethyleneglycol-carboxyl (DSPE-PEG-COOH) penetrates the monolayer phospholipid to serve as the shell. The phospholipid, the DSPE-PEG-COOH and the PLGA have good biocompatibility, can entrap hydrophobic drugs and control the drugs to release slowly in the human bodies. The phospholipid surrounds the surface of the PLGA, thus ensuring that the particle can avoid immune system recognition so that the circulating half life of the particle is lengthened. The PEG shell ensures the particle to have spatial stability, static stability, long circulation and other characteristics and to be not easily agglomerated. Meanwhile, carboxyl is easily crosslinked with such ligands as antibodies, peptide, probes and the like, thus ensuring the particle to have targeting characteristic. The preparation method of the nanoparticle is simple, convenient practical and is easy to operate and popularize.

The embodiment of the invention provides an intelligent amphiphilic polymer nano micelle which comprises components of targeted peptide-polyethylene glycol-b-polyhistidine-b-polycysteine-phenylpropionic acid, wherein targeted peptide is a polypeptide sequence capable of mediating an active targeting transferring function, a polyethylene glycol chain segment is amino-polyethylene glycol-carboxyl, a carboxyl terminal of the polyethylene glycol chain semgnet is connected with the targeted peptide, an amino terminal of the polyethylene glycol is connected with a polyhistidine chain segment, the other terminal of the polyhistidine chain segment is connected with a polycysteine chain segment, and the phenylpropionic acid as a terminal capping reagent is connected with amino at the tail terminal of the polycysteine chain segment. The intelligent amphiphilic polymer nano micelle has better biocompatibility, can be biologically degraded and metabolized, has a pH sensitive response and an active targeting transferring function, can be used as a hydrophobic drug carrier, and can also load gold nanoparticles to realize photo-thermal treatment of tumors. The embodiment of the invention further provides a preparation method and application of the intelligent amphiphilic polymer nano micelle.

The invention relates to a nano drug delivery system containing polymer and phospholipid. The system comprises polylactic-co-glycolic acid, phospholipid, drug and linear polymer of distearoyl phosphatidyl ethanolamine, polyethylene glycol and folic acid, wherein the drug is coated by the polylactic-co-glycolic acid to form a core; the phospholipid surrounds the surface of the polylactic-co-glycolic acid to form an intermediate layer; and the linear polymer of the distearoyl phosphatidyl ethanolamine, the polyethylene glycol and the folic acid uses the distearoyl phosphatidyl ethanolamine to interleave in the phospholipid intermediate layer to form an outer layer to provide a shell of the polyethylene glycol and a targeting ligand of the folic acid. The Nano drug delivery system containing polymer and phospholipid has comprehensive advantages of the polymer and a nano-liposome, has the advantages of good biocompatibility, controllable biodegradability and low toxicity of degradation products, capacities of easily realizing targeted controlled release and achieving surface functionalization, and the like. In addition, the invention further provides a preparation method of the nano drug delivery system containing the polymer and the phospholipids, which is simple and convenient to operate.

The invention discloses a targeted oxygen-carrying nano sonosensitizer and a preparation method thereof, and the targeted oxygen-carrying nano sonosensitizer is prepared by hybridizing human serum albumin (HSA) and oxygen-carrying hemoglobin (Hb) by an ultrasonic method and wrapping hydrophobic sonosensitizer manganese porphyrin (MnPP). The method is simple in preparation process, and the preparedtargeted oxygen-carrying nano sonosensitizer is stable in property, high in bioavailability and good in targeting property, and can efficiently deliver exogenous oxygen molecules and MnPP into tumorsin a targeting manner, so that the tumor anoxic microenvironment is improved, and the sonodynamic curative effect is improved.

The invention belongs to the technical field of aquaculture, and relates to the technology of culture of oyster parents of patinopecten yesoensis, in particular to a keep-alive dry transportation method for the oyster parents of patinopecten yesoensis. The method comprises the steps that healthy patinopecten yesoensis reaching the sexual maturity ages and carrying no pathogenic bacteria is selected as the oyster parents, and the oyster parents are loaded into a heat preservation box after impurities on the surfaces of the oyster parents are washed away and removed; the loading quantity is controlled according to the specification of the oyster parents; the loading quantity of the oyster parents accounts for 73% to 77% of the volume of the heat preservation box, and bottled freezing sea water, wet sea grass and cotton cloth or linen and other covering materials with good water absorption performance and the large superficial area are placed in the heat preservation box; the moisture absorption covering materials account for 12% to 18%, and air space accounting for 5% to 15% of the volume of the heat preservation box is additionally reserved; the heat preservation box is placed in a low-temperature environment at the temperature 2 DEG C to 5 DEG C to be transported after being sealed by adhesive tape, and the heat preservation box is placed in low-temperature water at the temperature of 4 DEG C for buffer recovery after reaching a destination. The method is simple in process, low in packaging and transporting cost and convenient to operate and popularize, the keep-alive transportation time reaches 30 hours, the survival rate reaches 99% or above, and long-distance keep-alive transportation of the oyster parents is achieved. The transportation problem of southward moving juvenile growing of shellfishes is effectively solved, and great significance is achieved on promoting upgrading and low-carbon, energy saving, healthy and sustainable development of the patinopecten yesoensis industry.

The invention discloses nanoparticles for tumor photothermal therapy and medical imaging, provides a preparation method of polyethylene glycol-IR-797-DC cell membrane nanoparticles (DC-NPs) capable of promoting enrichment, proliferation, activation and near-infrared light absorption of T cells at a tumor site and formed by coating particle skeletons formed by phospholipid polyethylene glycol amine (DSPE-PEG) and near-infrared light triggered IR-797 with a DC cell membrane loaded with an antigen, and aims to synergistically improve the low-temperature photothermal antitumor treatment effect by enhancing the immune response of the body.

The invention discloses an off-line cleaning method of filter press nylon filtration fabric based on iron ore concentrate slurry dehydration blocking invalidation, combining physical cleaning with chemical cleaning to lighten damage to filtration fabric nylon yarn and lower the filtration fabric consumption and product cost of iron ore mineral dressing. The off-line cleaning method specifically comprises the following steps: 1) physical cleaning: taking high-pressure water jet flow of 50-150 kg/cm2, which is provided by a plunger pump, to carry out double-face scanning-type cleaning to blocking filtration fabric; 2) chemical cleaning: dipping and reacting for 0.5-4 hours in chemical cleanout fluid composed of water, inorganic acid, accelerator, penetrant and corrosion inhibitor; and 3) surface reinforcement: carrying out surface reinforcement on the filtration fabric in diphenylamine or triethanolamine solution of which the concentration is 0.05-5%.

The invention belongs to the technical field of aquaculture, relates to a cultivation technique of parent scallops of patinopecten yessoensis, and in particular relates to a water transportation method for ensuring living parent scallops of patinopecten yessoensis. The method comprises the following steps: selecting healthy patinopecten yessoensis at the age of sexual maturity and without pathogenic bacteria as parent scallops, washing for removing impurities on the surfaces of the parent scallops, emptying excrement in the parent scallops, putting the parent scallops into a water tank in an airtight heat-insulation cabin, transporting the parent scallops to a destination in a low-temperature environment at 2-5 DEG C, and putting the parent scallops in a fostering pond at the water temperature of 3-7 DEG C for buffering and temporary keeping. By virtue of the method, the living transportation time reaches 72 hours, and the survival rate of the parent scallops is up to 99% and above. According to the method, the process is simple, the transportation cost is low, the equipment can be reused, and the operation and popularization are facilitated. The method can be used for effectively solving the bottleneck problem of high death rate during long distance transportation in processes of cultivating of fry of patinopecten yessoensis, introduction of fine varieties from abroad and marketing, and thus has important significance in promoting upgrading and low-carbon and energy-saving healthy sustainable development of industry of patinopecten yessoensis.

The invention relates to a method for real-time monitoring on gene transfection. Based on polyethyleneimine marked by tetramethyl isothiocyanic acid rhodamine, polyethyleneimine is subjected to tetramethyl isothiocyanic acid rhodamine marking at a room temperature. The method has the advantages of simplicity of synthesis, moderate conditions, low cost and convenience for operation. Plasmid DNA (Deoxyribonucleic Acid) of a fluorescent protein is expressed by electrostatic adsorption of the polyethyleneimine marked by the tetramethyl isothiocyanic acid rhodamine, and the plasmid DNA is transfected into cells; a fluorescence imaging method is used for monitoring the distribution of the polyethyleneimine which is used as a carrier and a plasmid in the cells and the expression of the plasmid in the cells in real time; and the method is simple and easy to realize, is suitable for most of laboratories and is convenient for operation and popularization.

The invention provides a preparation method of a carbon-based metal catalyst, comprising: preparing a carbon-based carrier, and then using a chelating agent to achieve effective combination of the carrier and a metal catalyst so as to prepare the carbon-based metal catalyst. The invention also provides a carbon-based metal catalyst and application of the carbon-based metal catalyst to removing gaseous benzene pollutants. The preparation process of the invention is simple and is easy to operate and promote. The prepared carbon-based metal catalyst has high dispersity, high capacity, good stability and long service life, can highly selectively remove gaseous benzene pollutants at low temperature, and can be widely applied to air purifiers, air conditioners, gas masks and ventilators to improve the selective removal of gaseous benzene pollutants and reduce pollution and harm to the environment and human body.

The invention discloses a galvanized iron box equipped with magnet, belonging to the field of file box technique. The box overcomes shortcomings in current technique, and is prepared manually with galvanized iron sheet. The inner box and outer cover are equipped with lock device which is connected with movable hinge, the inner box of iron box is provided with four permanent magnets, one iron sheet cover board is located on the permanent magnet, the length of inner box is 30. 5 cm, width is 21. 5 cm and height is 4 cm. The patent file and letter from Patent Agency can be put into inner box, and can be separated according to department or overlapped when there are many file boxes. The permanent magnet can adsorb iron sheet cover and the box can not move. The iron box can be booked, and is characterized by simple production, long life length, low cost, easy for spread and good practicality.

The invention discloses a method for constructing a mixed sowing grassland by reseeding alfalfa in a slightly degraded leymus chinensis meadow, and relates to a method for constructing an artificial grassland in a degraded leymus chinensis meadow. The invention aims to improve the community structure of the leymus chinensis meadow and improve the yield and quality of forage grass. The method comprises the following steps that 1, in early July, the slightly degraded leymus chinensis meadow is cut, stubbles of 3-5 cm are reserved, and light raking operation is conducted; 2, after light raking operation, alfalfa seeds are sown, the sowing rate is 10 kg/hectare, and after sowing, a compacting machine is used for slight compacting; 3, in the current year of alfalfa sowing, the grassland is forbidden to be cut, the grassland is cut twice every year from the next year, the cutting time is selected at the initial flowering stage of alfalfa each time, and after the first cutting, the grassland is fertilized. According to the method, the yield of high-quality forage grass can be increased by 1.5-2 tons/hectare per hectare, the price of the forage grass is 1000 yuan/ton, the income can be increased by at least 1500 yuan/hectare every year, and the economic benefit in the later period is very considerable. The invention belongs to the field of degraded grassland recovery.

The invention provides nanometer medicine-loaded micelle, a nanometer anticancer medicine, methods for preparing the nanometer medicine-loaded micelle and the nanometer anticancer medicine and application of the nanometer medicine-loaded micelle and the nanometer anticancer medicine. The nanometer medicine-loaded micelle comprises hyaluronic acid and dye IR808. The dye IR808 is connected onto the hyaluronic acid by adipic dihydrazide groups. The hyaluronic acid is used as a hydrophilic shell for the nanometer medicine-loaded micelle, and the dye IR808 is used as a hydrophobic core for the nanometer medicine-loaded micelle, so that a shell-core structure can be formed. The nanometer medicine-loaded micelle, the nanometer anticancer medicine, the methods and the application have the advantages that tumor cell targeting, imaging and photothermal therapy effects can be realized by the nanometer medicine-loaded micelle; the nanometer medicine-loaded micelle can be used as a medicine carrier and can wrap hydrophobic anticancer medicines, so that the nanometer anticancer medicine can be formed, joint therapy effects of chemical medicine therapy and photothermal therapy can be realized, and curative effects can be improved for tumor; the methods for preparing the nanometer medicine-loaded micelle and the nanometer anticancer medicine are simple, and accordingly operation and popularization can be facilitated.

The invention discloses an anti-UV and antibacterial extinction chinlon chip and a preparation method thereof, and belongs to the technical field of textile, the anti-UV and antibacterial extinction chinlon chip comprises a titanium dioxide matting agent, nano zinc oxide composite particles and caprolactam; and the nano zinc oxide composite particles comprise nano-zinc oxide particles, and the peripheries of the nano-zinc oxide particles are coated with organic silicon TEOS-28. The preparation method comprises the following steps: preparing a blending solution from a titanium dioxide delustering agent and nano-zinc oxide composite particles, mixing the blending solution with acid, amine and caprolactam, and carrying out pre-polymerization, final polymerization, filtration, granulation, extraction, drying and cooling to obtain a finished product. The preparation method is low in cost input and easy to control, the used raw materials are not easy to agglomerate and disperse, and the effects of the raw materials can be fully exerted; and the anti-UV, antibacterial and matting chinlon chip is used for preparing fibers or further used for weaving, and can endow the fibers and cloth with good anti-UV, antibacterial and matting properties.

The invention discloses a liquid feed for growing and fattening pigs. The liquid feed is prepared by fermenting Xinhui citrus pulp and bean dregs through a compound bacterium-enzyme preparation solution, The liquid feed raw material comprises the following components in parts by mass: 20-50 parts of a mixture of fermented citrus juice and bean dregs, 10-35 parts of corn flour, 5-10 parts of barley, 8-15 parts of bran coat, 0.35-0.7 part of edible salt, 1-3 parts of bone meal, 5-10 parts of soybean oil and 1-5 parts of a premix for growing and fattening pigs. According to the liquid feed, agricultural processing byproducts of the Xinhui citrus pulp and the bean dregs are utilized, digestive enzymes and active bacteria are added for fermentation, nutrient substances of the raw materials areexerted, the liquid feed has the functions of remarkably increasing the average daily gain and average daily feed intake of growing and fattening pigs and reducing the morbidity of the growing and fattening pigs, and the utilization rate of the Xinhui citrus pulp and the bean dregs is increased.

The invention discloses a targeting PLGA (poly(lactic-co-glycolic acid)) fluorescence probe coated with methylene blue, wherein, the targeting PLGA fluorescence probe uses methylene blue as a core and PLGA as a shell membrane, and PLGA is cross-linked with folic acid molecules through 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimides; the targeting PLGA fluorescence probe is targeting PLGA nanoparticles coated with methylene blue; and PLGA has good biocompatibility and biodegradability and can be discharged out of body though normal physiological pathways. Light stability of methylene blue is enhanced after methylene is coated on PLGA; at the same time, the particles crosslinked with folic acid can recognize tumors in vivo, has low preparation cost and is convenient in operation and popularization.