ICG-encapsulated polymer-phospholipid nano-particle and preparation method thereof

A nanoparticle and polymer technology, which can be applied to medical preparations without active ingredients, medical preparations containing active ingredients, and drug combinations, etc., can solve the problems of small preparation dose, achieve stable properties, and simple and easy preparation methods. , the effect of simple operation

Active Publication Date: 2015-05-06
ZHUHAI INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The polymer-phospholipid nanoparticles loaded with ICG in the present invention are prepared by a one-step high-pressure homogenization method, the preparation method is simple, and a large dose of polymer-phospholipid nanoparticles can be prepared, which solves the problem of the preparation dosage of polymer-phospholipid nanoparticles in the prior art minor problem

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  • ICG-encapsulated polymer-phospholipid nano-particle and preparation method thereof

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Embodiment 1

[0029] A preparation method of polymer-phospholipid nanoparticles loaded with ICG, comprising the following steps:

[0030] (1) Dissolving soybean lecithin and ICG in 4% ethanol aqueous solution to obtain a mixed solution, the concentration of soybean lecithin in the mixed solution is 0.15mg / mL, the concentration of ICG is 0.2mg / mL, and the total volume is 40mL ; Keep the mixed solution at a temperature of 90° C., adjust the pressure to 500 bar, and initially emulsify for 2 minutes to obtain an emulsion;

[0031] (2) Dissolve polymer PLGA with a molecular weight of 5000 in acetocyanide to obtain a PLGA solution with a concentration of 1 mg / mL, with a total volume of 10 mL; add the PLGA solution dropwise to the emulsion, keep the temperature at 90°C, and keep the pressure at 500 bar, The emulsification was continued for 4 minutes to form a 50 mL solution containing ICG-loaded PLGA-phospholipid nanoparticles to obtain ICG-loaded PLGA-phospholipid nanoparticles.

[0032] figure...

Embodiment 2

[0035] A preparation method of polymer-phospholipid nanoparticles loaded with ICG, comprising the following steps:

[0036] (1) soybean lecithin and ICG are dissolved in the ethanol aqueous solution of 4% to obtain mixed solution in mass concentration, the concentration range of soybean lecithin in the mixed solution is 0.75mg / mL, ICG concentration is 1.5mg / mL, and total volume is 40mL; keep the mixed solution at a temperature of 60°C, adjust the pressure to 1400bar, and emulsify initially for 2 minutes to obtain an emulsion;

[0037] (2) Dissolve polymer PLGA with a molecular weight of 15,000 in acetocyanide to obtain a PLGA solution with a concentration of 5 mg / mL, with a total volume of 10 mL; add the PLGA solution dropwise to the emulsion, keep the temperature at 60 °C, and the pressure at 1400 bar, The emulsification was continued for 6 minutes to form a 50 mL solution containing ICG-loaded PLGA-phospholipid nanoparticles to obtain ICG-loaded PLGA-phospholipid nanoparticl...

Embodiment 3

[0039] A preparation method of polymer-phospholipid nanoparticles loaded with ICG, comprising the following steps:

[0040] (1) Dissolving soybean lecithin and ICG in 4% ethanol aqueous solution to obtain a mixed solution, the concentration of soybean lecithin in the mixed solution is 0.15mg / mL, the concentration of ICG is 0.2mg / mL, and the total volume is 800mL ; Keep the mixed solution at a temperature of 80°C, adjust the pressure to 500bar, and initially emulsify for 2 minutes to obtain an emulsion;

[0041] (2) Dissolve polymer PLGA with a molecular weight of 10,000 in acetonitrile to obtain a PLGA solution with a concentration of 1 mg / mL, with a total volume of 200 mL; add the PLGA solution dropwise to the emulsion, keep the temperature at 80°C, and the pressure at 500 bar, The emulsification was continued for 4 minutes to form a 1000 mL solution containing ICG-loaded PLGA-phospholipid nanoparticles to obtain ICG-loaded PLGA-phospholipid nanoparticles. The particle size ...

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Abstract

The invention provides an ICG-encapsulated polymer-phospholipid nano-particle. The ICG-encapsulated polymer-phospholipid nano-particle comprises a kernel and a shell, wherein the kernel is formed by coating the surface of ICG with a polymer, the shell is phospholipid coating the surface of the polymer, and the polymer is polyglycollide lactide or polylactic acid. The ICG-encapsulated polymer-phospholipid nano-particles are uniform in particle size and stable in performance. The invention further provides a preparation method of the ICG-encapsulated polymer-phospholipid nano-particle. According to the preparation method, the ICG-encapsulated polymer-phospholipid nano-particle is prepared by virtue of a one-step high pressure homogenation method. The preparation method is simple and can be utilized for preparing a large dose of the ICG-encapsulated polymer-phospholipid nano-particles.

Description

technical field [0001] The invention relates to the field of drug carriers, in particular to a polymer-phospholipid nanoparticle carrying ICG and a preparation method thereof. Background technique [0002] The two mainstream nanocarriers represented by polymer nanoparticles and nanoliposomes can efficiently package and transport drugs or genes, etc., and have become research hotspots of scientists from all over the world. Polymer nanoparticles have the advantages of strong drug encapsulation ability, high cell endocytosis efficiency, and long circulation time in vivo. Nanoliposomes have the characteristics of excellent biological safety, high transport capacity, and simple preparation process. Polymer-phospholipid nanoparticles prepared by combining the respective advantages of polymers and phospholipids have been widely used in drug delivery. However, the existing preparation methods of polymer-phospholipid nanoparticles are mainly ultrasonic method and nano-precipitation...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K9/14A61K47/34A61K47/24A61P35/00
CPCA61K9/14A61K47/24A61K47/34
Inventor 蔡林涛郑明彬陈泽罗震宇赵鹏飞龚萍
Owner ZHUHAI INST OF ADVANCED TECH
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