Preparation and application of nano material for specifically activating immune system

A nanoparticle and dye technology, which is applied to preparations for in vivo experiments, medical preparations with inactive ingredients, and medical preparations containing active ingredients, etc., to achieve easy operation and promotion, promote photothermal treatment effects, and simple preparation methods. easy effect

Inactive Publication Date: 2021-05-07
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the prior art, there are few studies on the application of the new organic photothermal material IR-797 in photothermal therapy. Compared with the traditional photothermal material indocyanine green, IR-797 has a more excellent photothermal therapy effect , the characteristics of high contrast and strong tissue penetrating biological near-infrared fluorescence imaging and photoacoustic imaging can be used for the diagnosis of tumors before treatment, the tracking during treatment and the evaluation of curative effect after treatment

Method used

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  • Preparation and application of nano material for specifically activating immune system
  • Preparation and application of nano material for specifically activating immune system
  • Preparation and application of nano material for specifically activating immune system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Preparation of antigen-loaded DC membrane-wrapped IR-797 nanoparticles (DC-NPs)

[0041] Using phospholipid polyethylene glycol (DSPE-PEG) as the nanoparticle skeleton to prepare membrane-wrapped nanoparticles, and at the same time encapsulate IR-797 for NIR-triggered photothermal therapy and imaging, the preparation flow chart is attached figure 1 As shown, it specifically includes the following steps:

[0042] (1) Dissolve 2mg DSPE-PEG and 2mg IR-797 in 2ml chloroform, pipette and mix well, transfer the chloroform mixture to the bottom of a beaker filled with 15ml distilled water, feed nitrogen into the bottom of the beaker, blow off the chloroform, and make the IR- Due to the hydrophobic interaction, the lipophilic groups in DSPE-PEG are combined to form nanoparticles, and the resulting solution is DSPE-PEG / IR-797 solution.

[0043] (2) Lyse the DC cells with lysate, sonicate for 10 minutes, then centrifuge at 12,000g for 20 minutes to remove insoluble mat...

Embodiment 2

[0046] Characterization of Example 2DC-NPs

[0047] The morphology and structure of DC-NPs nanoparticles were observed by transmission electron microscopy. The zeta potential instrument analyzed the charge change of nanoparticles before and after DC cell membranes were encapsulated, and the particle size analyzer analyzed the particle size distribution of nanoparticles before and after DC cell membranes were encapsulated. The results are as follows Figure 2-3 As shown, the DC cell membrane was successfully wrapped on the surface of the nanoparticles, and the hydrated particle size of DC-NPs was 86±4nm. Fluorescence spectrometer was used to detect the fluorescence spectrum of DC-NPs nanoparticles to determine the imaging conditions, and UV-Vis absorption spectrometer was used to detect the UV-Vis absorption spectrum of nanoparticles to determine the laser wavelength for particle photothermal treatment. The results were as follows: Figure 4-5 As shown, the ultraviolet-visible ...

Embodiment 3

[0048] The photothermal treatment of embodiment 3DC-NPs

[0049] Female BALB / c (4-6 weeks, body weight 15-20g) subcutaneously injected 1×10 6 4T1 breast cancer cells. The formula for calculating tumor volume is: tumor length × (tumor width) 2 / 2. When the tumor volume reaches 100-200mm 3 When left or right, 200 μL of DC-NPs (IR-797 concentration of 100 μg / mL) was injected intravenously, and 0.3 W / cm 2 The power density of 808nm near-infrared laser was irradiated for 30 minutes, and the temperature of the irradiated part was maintained at 42°C.

[0050] 0.3W / cm 2 The power density of the 808nm near-infrared laser treated the tumor-bearing mice in the non-injected DC-NPs group for 15 days, and the tumor grew to about 1350mm 3 , while DC-NPs were treated with laser irradiation for 20 days, and the tumors were basically eliminated.

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Abstract

The invention discloses nanoparticles for tumor photothermal therapy and medical imaging, provides a preparation method of polyethylene glycol-IR-797-DC cell membrane nanoparticles (DC-NPs) capable of promoting enrichment, proliferation, activation and near-infrared light absorption of T cells at a tumor site and formed by coating particle skeletons formed by phospholipid polyethylene glycol amine (DSPE-PEG) and near-infrared light triggered IR-797 with a DC cell membrane loaded with an antigen, and aims to synergistically improve the low-temperature photothermal antitumor treatment effect by enhancing the immune response of the body.

Description

technical field [0001] The invention relates to the field of biological nanomaterials, in particular to an antigen-loaded DC cell membrane-wrapped nanoparticle and a nanometer delivery carrier containing the nanoparticle. Background technique [0002] Cancer, namely malignant tumor, has become one of the important diseases threatening human health all over the world. The existing mainstream cancer treatment methods include surgical resection, chemotherapeutic drugs (chemotherapy), and radiotherapy, but these treatments have their own limitations. Although surgical resection has a good therapeutic effect on early solid tumors, it has a The therapeutic effect of metastatic tumors is limited. In addition, in order to ensure the curative effect, a large number of normal tissues adjacent to the tumor are often removed during the operation, which causes obvious damage to the patient's body; chemotherapy is a way of administering drugs through the system It is a commonly used clin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K9/50A61K35/15A61K39/00A61K47/24A61K47/46A61K49/00A61K49/22A61P35/00
CPCA61K9/5068A61K35/15A61K39/0011A61K41/0052A61K47/24A61K49/0032A61K49/0097A61K49/225A61K2039/5154A61K2039/585A61P35/00A61K2300/00A61K9/16A61K9/50A61K39/00A61K41/00A61K47/46A61K49/00A61K49/22
Inventor 蔡林涛孙枝红邓冠军龚萍
Owner SHENZHEN INST OF ADVANCED TECH
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