Targeting PLGA (poly(lactic-co-glycolic acid)) fluorescence probe coated with methylene blue and the preparation method thereof

Abstract

The invention discloses a targeting PLGA (poly(lactic-co-glycolic acid)) fluorescence probe coated with methylene blue, wherein, the targeting PLGA fluorescence probe uses methylene blue as a core and PLGA as a shell membrane, and PLGA is cross-linked with folic acid molecules through 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimides; the targeting PLGA fluorescence probe is targeting PLGA nanoparticles coated with methylene blue; and PLGA has good biocompatibility and biodegradability and can be discharged out of body though normal physiological pathways. Light stability of methylene blue is enhanced after methylene is coated on PLGA; at the same time, the particles crosslinked with folic acid can recognize tumors in vivo, has low preparation cost and is convenient in operation and popularization.

Description

technical field [0001] The invention belongs to the field of nanomedicine, and relates to a preparation method of methylene blue nanoparticles, in particular to a targeting PLGA fluorescent probe wrapped with methylene blue and a preparation method thereof. Background technique [0002] Based on the threat of cancer in human health and the advantages of using in vivo fluorescence imaging to diagnose tumor tissues, the development of effective imaging agents has become an important aspect of current scientific research and clinical research. Most of the imaging probes commonly used in in vivo fluorescence imaging are traditional organic dye molecules, such as methylene blue, rhodamine, FITC, and Cy3, and these organic dye molecules have some shortcomings of their own, such as poor photostability and sensitivity. Low, strong hydrophobicity and poor targeting, thus limiting its further application. The development of fluorescent nanoprobes that can solve the above problems is ...

AI Technical Summary

Problems solved by technology

Most of the imaging probes commonly used in in vivo fluorescence imaging are traditional organic dye molecules, such as methylene blue, rhodamine, FITC, and Cy3, and these organic dye molecules have some shortcomings of their own, such as poor photostability and sensitivity. low, strong hydrophobicity and poor targeting, thus limiting its further application

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