Preparation method of yak bone collagen peptide for increasing bone mineral density and yak bone collagen peptide

Through the synergistic action of complex bacteria and complex enzymes, yak bones are efficiently degraded and small molecular weight collagen peptides are extracted, which solves the problems of poor extraction efficiency and quality in the prior art, and improves bone density and bone calcium.

CN119753072AInactive Publication Date: 2025-04-04DEZHOU LANLI BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202510266371.2
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-03-07
Publication Date
2025-04-04
Estimated Expiration
Not applicable · inactive patent

AI Technical Summary

Technical Problem

It is difficult to efficiently extract high-quality yak bone collagen peptides, especially when the mineral content in yak bones is high. Common acid hydrolysis methods and enzyme methods have problems of degradation or deformation, resulting in poor extraction efficiency and quality.

Method used

The method of synergistic fermentation of complex bacteria (Lactobacillus plantarum and propionate) and synergistic lysis of complex enzymes (trypsin, alkaline protease and flavor protease) is adopted to efficiently degrade yak bones through the synergistic action of microbial metabolism, promote calcium absorption and utilization, and decompose collagen into small molecule peptides to improve the extraction rate and quality.

Benefits of technology

It has achieved efficient extraction of more small molecular weight collagen peptides, which has increased bone density and bone calcium content, and enhanced bone strength and density.

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Abstract

The invention discloses a preparation method of yak bone collagen peptide for increasing bone mineral density and the yak bone collagen peptide. The invention provides a preparation method of yak bone collagen peptide for increasing bone mineral density. The preparation method comprises the following steps: providing yak bone powder; mixing the yak bone meal with a phosphate buffer solution, adjusting the pH value to 5-5.5, mixing with compound bacteria, and carrying out demineralization treatment to obtain a demineralized product; mixing the demineralized product with a compound enzyme, and carrying out an enzymolysis reaction to obtain an enzymolysis product; heating, centrifugally filtering and freeze-drying the enzymolysis product to prepare yak bone collagen peptide; wherein the compound enzyme comprises trypsin, alkaline protease and flavourzyme, and the compound bacteria comprise lactobacillus plantarum and propionibacterium. Lactobacillus plantarum and propionibacterium in the compound bacteria are subjected to synergistic fermentation, and are combined with the enzymolysis reaction of the compound enzyme, so that collagen is decomposed into small molecule peptides more efficiently, the extraction rate of bone collagen peptide is increased, and the bone mineral density is increased.
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