The multi-channel modular animal inhalation exposure box comprises a test box, a side door is hinged to a side opening of the test box, a mist inlet, a detection port, an air inlet and an air outlet are formed in the test box, and experimental animals are placed in the test box. The multi-channel modularized animal inhalation exposure box is not only suitable for contamination experiment research with controllable dosage and condition in a laboratory, but also suitable for outdoor exposure research in an actual environment, that is, experimental animals can be exposed to atomized aerosol particles, simulated contamination gas, tail gas, flue gas and the like, and can also be exposed to an external actual atmospheric environment; the animal exposure system is simple in structure and convenient to use, simultaneous exposure research on multiple groups of animals, different target pollutants and different contamination doses can be achieved through modular combination, the exposure system can achieve the purpose of actual environment exposure or specific environment exposure through natural respiration intake of the animals, and convenience and diversity of research are achieved.