Preparation method and application of silkworm pupa protein antioxidant peptides
A silkworm chrysalis protein and antioxidant peptide technology, which is applied in the application of silkworm chrysalis protein antioxidant peptides and the preparation of silkworm chrysalis protein antioxidant peptides, can solve the problems of direct utilization of silkworm chrysalis protein, poor water solubility, and the improvement of the added value of silkworm chrysalis
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[0025] The invention provides a preparation method of silkworm chrysalis protein antioxidant peptide, comprising the following steps:
[0026] 1) mixing silkworm chrysalis powder with petroleum ether, ultrasonic degreasing, solid-liquid separation, and drying the obtained solid phase to obtain degreased silkworm chrysalis powder;
[0027] 2) mixing the defatted silkworm chrysalis powder in the step 1), water and silkworm chrysalis proteolytic enzyme for enzymolysis to obtain silkworm chrysalis powder enzymatic hydrolysis;
[0028] 3) Inactivate the silkworm chrysalis powder enzymatic hydrolyzate in step 2), centrifuge, filter the obtained supernatant with a 0.40-0.50 μm filter membrane, and collect the filtrate;
[0029] 4) The filtrate in the step 3) is ultrafiltered with a 3000Da ultrafiltration membrane, and the permeate is collected to obtain an ultrafiltrate;
[0030] 5) The ultrafiltrate in the step 4) is ultrafiltered with a 200Da ultrafiltration membrane, and the rete...
Embodiment 1
[0050] Weigh 100g of silkworm chrysalis powder with a particle size of 140 mesh, wrap it with filter paper, put it into a glass container, add 600mL of petroleum ether with a boiling range of 60-90°C into the glass container, and ultrasonically degrease it 4 times at 100W and 45°C. 30 minutes each time, after ultrasonication, the silkworm chrysalis powder wrapped in filter paper was taken out, dried at 50°C for 60 minutes to obtain degreased silkworm chrysalis powder, and the obtained defatted silkworm chrysalis powder was crushed to 190 mesh again, and the defatted silkworm chrysalis powder, water and silkworm chrysalis protein were hydrolyzed The enzymes were mixed according to the mass ratio of 5:105:2.5, and the resulting mixture was enzymolyzed at 50°C for 6h (the pH value was controlled at 7.0 during the enzymolysis process (the pH of the solution was checked every 25min, and when there was a change, 1mol / L hydrochloric acid or sodium hydroxide to adjust) and then inacti...
Embodiment 2
[0060] Weigh 100g of silkworm chrysalis powder with a particle size of 160 mesh, wrap it with filter paper, put it into a glass container, add 1000mL of petroleum ether with a boiling range of 60-90°C into the glass container, and ultrasonically degrease it twice at 150W and 35°C. 50 minutes each time, after ultrasonication, the silkworm chrysalis powder wrapped in filter paper was taken out, dried at 60°C for 45 minutes to obtain defatted silkworm chrysalis powder, and the obtained defatted silkworm chrysalis powder was crushed to 210 mesh again, and the defatted silkworm chrysalis powder, water and silkworm chrysalis protein were hydrolyzed The enzymes were mixed according to the mass ratio of 8:95:4, and the resulting mixture was enzymolyzed at 60°C for 4 hours (the pH value was controlled at 8.0 during the enzymolysis process (the pH of the solution was checked every 35min, and when there was a change, 1mol / L of hydrochloric acid or sodium hydroxide to adjust) and then ina...
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