Preparation method and application of silkworm pupa protein antioxidant peptides

A silkworm chrysalis protein and antioxidant peptide technology, which is applied in the application of silkworm chrysalis protein antioxidant peptides and the preparation of silkworm chrysalis protein antioxidant peptides, can solve the problems of direct utilization of silkworm chrysalis protein, poor water solubility, and the improvement of the added value of silkworm chrysalis

Inactive Publication Date: 2018-08-28
SOUTHWEST UNIV
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Problems solved by technology

[0004] Although silkworm chrysalis protein is a high-quality protein, its water solubility is poor due to its large molecular weight, which not only affects its application in the food industry, but also affects its digestion and absorption by the human body
Silkworm chrysalis protein also has macromolecular proteins of about 30kDa or more, which may cause allergic reactions in some people, which hinders the application of silkworm chrysalis protein in food, and also brings risks to the direct utilization of silkworm chrysalis protein
[0005] Moreover, since silkworm chrysalis silk reeling is usually carried out under high temperature and strong alkali, the silkworm chrysalis after silk reeling not only has a fishy smell that is difficult to get rid of, but also denatures protein and oxidizes fat to produce a serious rancid smell. It is difficult to accept these bad flavors. At present, silkworm chrysalis is mainly used as feed, and less of them are directly used as food raw materials, which affects the improvement of added value of silkworm chrysalis

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  • Preparation method and application of silkworm pupa protein antioxidant peptides
  • Preparation method and application of silkworm pupa protein antioxidant peptides
  • Preparation method and application of silkworm pupa protein antioxidant peptides

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[0025] The invention provides a preparation method of silkworm chrysalis protein antioxidant peptide, comprising the following steps:

[0026] 1) mixing silkworm chrysalis powder with petroleum ether, ultrasonic degreasing, solid-liquid separation, and drying the obtained solid phase to obtain degreased silkworm chrysalis powder;

[0027] 2) mixing the defatted silkworm chrysalis powder in the step 1), water and silkworm chrysalis proteolytic enzyme for enzymolysis to obtain silkworm chrysalis powder enzymatic hydrolysis;

[0028] 3) Inactivate the silkworm chrysalis powder enzymatic hydrolyzate in step 2), centrifuge, filter the obtained supernatant with a 0.40-0.50 μm filter membrane, and collect the filtrate;

[0029] 4) The filtrate in the step 3) is ultrafiltered with a 3000Da ultrafiltration membrane, and the permeate is collected to obtain an ultrafiltrate;

[0030] 5) The ultrafiltrate in the step 4) is ultrafiltered with a 200Da ultrafiltration membrane, and the rete...

Embodiment 1

[0050] Weigh 100g of silkworm chrysalis powder with a particle size of 140 mesh, wrap it with filter paper, put it into a glass container, add 600mL of petroleum ether with a boiling range of 60-90°C into the glass container, and ultrasonically degrease it 4 times at 100W and 45°C. 30 minutes each time, after ultrasonication, the silkworm chrysalis powder wrapped in filter paper was taken out, dried at 50°C for 60 minutes to obtain degreased silkworm chrysalis powder, and the obtained defatted silkworm chrysalis powder was crushed to 190 mesh again, and the defatted silkworm chrysalis powder, water and silkworm chrysalis protein were hydrolyzed The enzymes were mixed according to the mass ratio of 5:105:2.5, and the resulting mixture was enzymolyzed at 50°C for 6h (the pH value was controlled at 7.0 during the enzymolysis process (the pH of the solution was checked every 25min, and when there was a change, 1mol / L hydrochloric acid or sodium hydroxide to adjust) and then inacti...

Embodiment 2

[0060] Weigh 100g of silkworm chrysalis powder with a particle size of 160 mesh, wrap it with filter paper, put it into a glass container, add 1000mL of petroleum ether with a boiling range of 60-90°C into the glass container, and ultrasonically degrease it twice at 150W and 35°C. 50 minutes each time, after ultrasonication, the silkworm chrysalis powder wrapped in filter paper was taken out, dried at 60°C for 45 minutes to obtain defatted silkworm chrysalis powder, and the obtained defatted silkworm chrysalis powder was crushed to 210 mesh again, and the defatted silkworm chrysalis powder, water and silkworm chrysalis protein were hydrolyzed The enzymes were mixed according to the mass ratio of 8:95:4, and the resulting mixture was enzymolyzed at 60°C for 4 hours (the pH value was controlled at 8.0 during the enzymolysis process (the pH of the solution was checked every 35min, and when there was a change, 1mol / L of hydrochloric acid or sodium hydroxide to adjust) and then ina...

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Abstract

The invention provides a preparation method of silkworm pupa protein antioxidant peptides and belongs to the technical field of food processing. The preparation method comprises the following steps: 1) mixing a silkworm pupa powder and petroleum ether and performing ultrasonic treatment and solid-liquid separation; 2) mixing a degreased silkworm pupa powder, water and silkworm pupa proteolytic enzymes for enzymolysis; 3) performing enzyme deactivation on a silkworm pupa powder enzymatic hydrolysis solution, centrifuging, filtering the obtained liquid supernatant by adopting a filter membrane of 0.40-0.50 mum and collecting a filtrate; 4) performing ultra-filtration on the filtrate by adopting an ultra-filtration membrane of 3000 Da and collecting a permeate to obtain a ultra-filtrate; 5) performing ultra-filtration on the ultra-filtrate by adopting an ultra-filtration membrane of 200 Da and collecting an intercept object. In the silkworm pupa protein peptides prepared through the preparation method provided by the invention, the content of essential amino acids reaches about 43 percent, and the total antioxidant ability, the ability to scavenge superoxide anions (O2.), the abilityto scavenge .OH and the ability to scavenge DPPH are stronger.

Description

technical field [0001] The invention belongs to the technical field of food processing, and in particular relates to a preparation method of silkworm chrysalis protein antioxidant peptide and an application of silkworm chrysalis protein antioxidant peptide. Background technique [0002] At present, the cocoon production in China is about 600,000 tons, accounting for about 80% of the world. Silkworm chrysalis (Silkwormpupa) is the chrysalis of silkworm moth (Bombyx moilL.), silkworm chrysalis. The classic works have detailed records on the functions of silkworm chrysalis, and believe that silkworm chrysalis has the functions of promoting body fluid and quenching thirst, eliminating food and regulating qi, calming convulsions, strengthening yang, strengthening spleen and stomach, dispelling rheumatism, and long muscle antipyretic. [0003] The protein content in silkworm chrysalis is as high as 45% to 55%, mostly globulin, rich in 18 kinds of amino acids, of which the content...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34A23L33/18A61K38/01A61P39/06
CPCA23L33/18A23V2002/00A61K38/00C12P21/06A23V2250/55A23V2200/30
Inventor 黄先智丁晓雯沈以红
Owner SOUTHWEST UNIV
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