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Application of inonotus obliquus polysaccharide used as feed additive for litopenaeus vannamei

A technology of Inonotus obliquus polysaccharide and Inonotus obliquus, which is applied in the application, animal feed, animal feed and other directions, can solve problems such as the inability to improve immunity, and achieve the goal of promoting sustainable development, broadening application scope and reducing feed coefficient. Effect

Inactive Publication Date: 2013-09-11
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the prawn diet can meet the nutritional requirements of Litopenaeus vannamei for growth, it cannot improve its immunity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Weigh 100 kg of Litopenaeus vannamei basal feed (commercially available, Hessant Litopenaeus vannamei compound feed produced by Qingdao Changsheng Zhongke Aquatic Feed Co., Ltd.), add 50 g of Inonotus obliquus polysaccharide (commercially available, Zhejiang Fangge Pharmaceutical Co., Ltd. Co., Ltd.), after mixing evenly, use a SLX-80 twin-screw extruder to make pellet feed with a diameter of 1.0 mm, and dry it at 55 °C.

Embodiment 2

[0040] Weigh 100kg of Litopenaeus vannamei basal feed (commercially available, Hessente vannamei compound feed produced by Qingdao Changsheng Zhongke Aquatic Feed Co., Ltd.), add 150g of Inonotus obliquus polysaccharide (commercially available, Zhejiang Fangge Pharmaceutical Co., Ltd. Co., Ltd.), after mixing evenly, use a SLX-80 twin-screw extruder to make pellet feed with a diameter of 1.0 mm, and dry it at 55 °C.

Embodiment 3

[0042] Weigh 100kg of basic feed for Litopenaeus vannamei (commercially available, Hessian Litopenaeus vannamei compound feed produced by Qingdao Changsheng Zhongke Aquatic Feed Co., Ltd.), add 80g of Inonotus obliquus polysaccharide, mix well and use SLX-80 The feed pellets with a diameter of 1.0 mm were made by a twin-screw extruder and dried at 55 °C.

[0043] The preparation method of Inonotus obliquus polysaccharide is as follows:

[0044] (1) Strain activation: use Inonotus obliquus (commercially available, purchased from Xuzhou Normal University) as the strain, inoculate it on the slant strain medium at 27°C, and cultivate it for 250 hours to obtain the activated strain; The formulation of the seed medium is: 40 g / 100mL of malt extract, 4 g / 100mL of peptone, 10 g / 100mL of agar, and the pH value is 5.4-5.6.

[0045] (2) Liquid strain culture: transfer the activated strain into the liquid seed medium at 27°C, cultivate on a shaker for 4.5 days until the logarithmic phase...

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Abstract

The invention discloses an application of inonotus obliquus polysaccharide used as a feed additive for litopenaeus vannamei, and the addition amount of the inonotus obliquus polysaccharide in a litopenaeus vannamei basal feed is 500-1500 mg / kg. The fact that the inonotus obliquus polysaccharide is used as the additive for the litopenaeus vannamei feed can substantially raise the weight gain rate and the specific growth rate of the litopenaeus vannamei, substantially reduce the feed coefficient, meanwhile substantially increase lysozyme and alkaline phosphatase in serum of the litopenaeus vannamei and raise total antioxidant capacity of the litopenaeus vannamei, increase nitric oxide synthetase and superoxide dismutase in the serum, and reduce the content of malonaldehyde in the serum. The inonotus obliquus polysaccharide has an equivalent action effect to beta glucan, and is a new-type immunopotentiator, and has a broad application prospect in the aquaculture field of the litopenaeus vannamei.

Description

technical field [0001] The invention relates to the field of aquaculture, in particular to the application of an Inonotus obliquus polysaccharide as a feed additive for Litopenaeus vannamei. Background technique [0002] Litopenaeus vannamei, commonly known as Penaeus vannamei, is one of the three largest shrimp species in the world with the highest production of farmed shrimp. Because of its fast growth rate, low nutritional requirements, large shrimp meat content, delicious meat, and rich nutrition, it is favored by consumers. In recent years, with the rapid development of large-scale and intensive farming of Litopenaeus vannamei, the outbreak of breeding diseases has become more and more frequent. In order to control the occurrence of diseases, chemical drugs and antibiotics have been used in large quantities, resulting in drug residues, resistance Problems such as drug properties and environmental pollution seriously affect the health of consumers. Therefore, it is par...

Claims

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Application Information

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IPC IPC(8): A23K1/18A23K1/16
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