Detection method for obtaining material composition and total antioxidant activity of lonicera confusa
A technology of antioxidant activity and detection method, applied in the field of detection and analysis, can solve the problems of inability to meet the quality control of traditional Chinese medicine, lack of pharmacological activity research, unclear interaction, etc., and achieve the effects of easy promotion, simple and convenient operation, and high sensitivity
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Embodiment 1
[0044] A detection method for simultaneously obtaining material components (chlorogenic acid) and total antioxidant activity in Flos Japonica, comprising:
[0045] Preparation of the test solution: Accurately weigh 0.5g of the Jasmine Japonica sample into a 50mL Erlenmeyer flask, add 10mL of 60% ethanol, ultrasonicate at 65°C for 30min (power 300W), take 2.0mL of the supernatant in an EP tube, 10000r / Min centrifugation; precision pipette 1.0mL supernatant into a 25mL volumetric flask, constant volume, that is, the test solution.
[0046] Preparation of DPPH solution: Accurately weigh 2.0mg of DPPH standard substance, place it in a 50mL brown volumetric flask, add absolute ethanol to make up to the mark, shake well, store in dark place, and set aside.
[0047] Preparation of VC (vitamin C) standard solution: Accurately weigh 2.5mg of VC standard, place in a 50mL volumetric flask, add distilled water to the mark, shake well; accurately measure 2.5mL, 5.0mL, 7.5mL, 10.0mL standa...
experiment example 1
[0062] Orthogonal Test of Chlorogenic Acid Extraction Method in Experimental Example 1 Mountain Silver Flower
[0063] Taking extraction temperature (A), ethanol concentration (B), extraction time (C), and solid-liquid ratio (D) as independent variables, the experiment was designed as the response value of extraction rate of chlorogenic acid. The tests and results are shown in Table S1, Table S2 and Table S3.
[0064] Table S1 Orthogonal test factor levels for extraction process of chlorogenic acid from Jasmine Japonica
[0065]
[0066] Orthogonal test arrangement and results
[0067] Table S2 Experimental data
[0068]
[0069] Table S3 Analysis of variance
[0070]
[0071] From the results of the range analysis in Table S3, it can be seen that for the content of chlorogenic acid, the four factors affecting the extraction rate are in the order of C>B>A>D, and the optimal extraction condition is C2B2A2D1.
[0072] From the results of variance analysis in Table S...
experiment example 2
[0073] Experimental example 2 Determination of analysis conditions
[0074] 1) Preparation of DPPH ethanol solution:
[0075] Accurately weigh 2.0 mg DPPH, place it in a 50 mL brown volumetric flask, add absolute ethanol to the mark, shake well, and store in the dark to obtain a 0.04 mg / mL DPPH solution.
[0076] 2) Preparation of vitamin C series standard solutions:
[0077] Accurately weigh 2.5mg of VC standard product, place it in a 50mL volumetric flask, add distilled water to the mark, and shake well. Accurately measure 2.5mL, 5.0mL, 7.5mL, 10.0mL standard solutions, place them in 25mL volumetric flasks, add distilled water to dilute to the mark, and shake well for later use.
[0078] 3) Preparation of the test solution:
[0079] Accurately weigh 0.5g sample (passed through a No. 3 sieve) into a stoppered conical flask, add 10mL of 60% ethanol, ultrasonicate at 65°C for 30min, centrifuge at 10000r / min for 10min, take 1.0mL of the supernatant in a 25mL volumetric flask,...
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