Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of antioxidant sea-buckthorn polypeptide

A technology for oxidizing sea buckthorn and sea buckthorn seeds is applied in the field of preparation of anti-oxidative sea buckthorn polypeptides, which can solve the problems of less research and the like

Inactive Publication Date: 2017-01-11
安徽旺润生物科技有限公司
View PDF1 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Overseas studies on seabuckthorn mainly focus on the medicinal value of seabuckthorn, mainly seabuckthorn oil, seabuckthorn flavonoids, etc. There are few studies on seabuckthorn protein and seabuckthorn peptides

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Take 20g seabuckthorn seeds (protein content is 25%), wash, pour water, and soak for 5 hours, add 100ml distilled water at 40°C, break the plant cells for 10 minutes in a high-pressure homogenizer, and use 8.0mol / L HCl solution to control the pH value 2. Add 2g of pepsin, homogenize under high pressure for 10 minutes, cool to room temperature, centrifuge at 3000r / min for 5 minutes, inactivate the supernatant at 70°C for 50 minutes, concentrate under reduced pressure to obtain a concentrated peptide solution; add the centrifuged residue to 40°C 60ml of distilled water was used for 2 times of high-pressure homogenization, and the method was the same as the above steps; the combined polypeptide concentrate was spray-dried to obtain the sea-buckthorn seed polypeptide.

[0016] The above seabuckthorn seed polypeptide was formulated into a 20mg / ml solution, and the determination of the total antioxidant capacity (AOV) showed that when the polypeptide solution was at 20mg / mL, t...

Embodiment 2

[0018] Take 100g seabuckthorn seeds (protein content is 25%), wash, pour water, and soak for 5 hours, add 1000ml distilled water at 60°C, break the plant cells in a high-pressure homogenizer for 5 minutes, and use 8.0mol / L HCl solution to control the pH value 4. Add 20g of pepsin, homogenize under high pressure for 5 minutes, cool to room temperature, centrifuge at 3000r / min for 5 minutes, inactivate the supernatant at 90°C for 30 minutes, concentrate under reduced pressure to obtain a concentrated polypeptide solution; add the centrifuged residue to 60°C 500ml of distilled water was subjected to high-pressure homogenization twice, the method was the same as the above steps; the combined polypeptide concentrate was spray-dried to obtain seabuckthorn seed polypeptide.

[0019] The above seabuckthorn seed polypeptide was formulated into a 20mg / ml solution, and the determination of the total antioxidant capacity (AOV) showed that when the polypeptide solution was at 20mg / mL, the h...

Embodiment 3

[0021] Take 500g seabuckthorn seeds (protein content is 25%), wash, pour water, and soak for 6 hours, add 4000ml distilled water at 50°C, break the plant cells in a high-pressure homogenizer for 8 minutes, and use 8.0mol / L HCl solution to control the pH value 3. Add 75g of pepsin, homogenize under high pressure for 8 minutes, cool to room temperature, centrifuge at 3000r / min for 5 minutes, inactivate the supernatant at 80°C for 40 minutes, concentrate under reduced pressure to obtain a concentrated peptide solution; add the centrifuged residue to 50°C 2000ml of distilled water was used for 2 times of high-pressure homogenization, and the method was the same as the above steps; the combined polypeptide concentrate was spray-dried to obtain the seabuckthorn seed polypeptide.

[0022] The above seabuckthorn seed polypeptide was formulated into a 20mg / ml solution, and the determination of the total antioxidant capacity (AOV) showed that when the polypeptide solution was at 20mg / mL,...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method of antioxidant sea-buckthorn polypeptide. The preparation method comprises the following steps: performing cleaning and soaking treatment on sea-buckthorn seeds, and then performing enzymatic hydrolysis extraction for two times by using pepsase; subsequently decompressing, concentrating and drying to obtain the sea-buckthorn seed polypeptide. The determination of total antioxidant capacity (AOV) shows that the scavenging rate of hydroxy free radicals is as high as 91.35 percent, and the scavenging rate of superoxide anions is as high as 64.33 percent when the concentration of a polypeptide solution is 20mg / mL; the antioxidant sea-buckthorn polypeptide has high oxidation resistance and has a broad application prospect in aspects of antioxidant food and cosmetics.

Description

technical field [0001] The invention relates to the preparation of seabuckthorn polypeptides, in particular to a preparation method of antioxidative seabuckthorn polypeptides. Background technique [0002] Seabuckthorn is a precious crop with both medicinal and edible uses. Studies have shown that seabuckthorn contains vitamin C, carotene, vitamin E, vitamin B1, vitamin B2, folic acid and other vitamins, as well as rich flavonoids, proanthocyanidins and different rats. Li Su and other bioactive substances. In the past, the development of seabuckthorn resources was mainly concentrated on the extraction of fruit juice, seabuckthorn oil and total flavonoids, and the processed residues were generally discarded or treated as roughage. Although seabuckthorn seeds contain 25% to 28% protein, most of these proteins are wrapped by woody fibers, and the digestion and utilization rate of protein is very low. If these proteins can be effectively hydrolyzed and processed into small mol...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 宁发子张志强宁成勇
Owner 安徽旺润生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com