Kit for detecting total antioxidant capacity

Abstract

The invention relates to a kit for detecting total antioxidant capacity. The kit comprises an R1 reagent and an R2 reagent with a volume ratio of 11:1, wherein the R1 reagent comprises a liquid A and a liquid B with a volume ratio of 10:1; the liquid A comprises acetate and the balance of double distilled water; the liquid B comprises a complex TPTZ (tripyridyl-triazine), acid and the balance of double distilled water; and the R2 reagent comprises an oxidizing agent FeCl3 and the balance of double distilled water. According to the invention, by adopting an FRAP (Ferric Reducing Antioxidant Power) method, the total antioxidant capacity of a sample to be detected is detected, the total antioxidant capacity of a reaction organism can be reflected, and the antioxidant state can be well reflected compared with that of the single antioxidant measurement. By using a spectrophotometer, a microplate reader or fully automatic biochemical analyser, the kit is high in automation and is beneficial to the popularization of the daily inspection work. A measurement value is high in dependency degree with in vivo test data. The kit is low in manufacture cost.

Description

technical field [0001] The invention relates to a kit, in particular to a kit for detecting total antioxidant capacity. Background technique [0002] Free radicals, also called "free radicals" in chemistry, are atomic groups containing one unpaired electron. With the development of free radical theory and biomedicine, the relationship between free radicals and diseases has received extensive attention. Studies have shown that free radicals induce oxidative stress, which is involved in the pathological process of many diseases. Excessive free radicals in the body will damage proteins and cell membranes, and promote DNA mutations in cell tissues, thereby inducing or accelerating the occurrence and deterioration of various diseases in the human body. In many cases such as radiation injury, inflammation and emergency response, tumor lesion, reperfusion injury, aging, etc., it is often accompanied by abnormal and sharp increase of free radicals. A large number of studies have ...

AI Technical Summary

Problems solved by technology

In vitro experiments often detect a single antioxidant component, which is difficult to accurately reflect the actual anti-oxidative stress ability of the organism, because the change of a single component cannot fully explain the occurrence and degree of oxidative damage

Assessing the body's true antioxidant capacity is difficult because the body inhibits different oxides with varying efficiencies