Astaxanthin nanoliposome and preparation method and application thereof

A technology of nano-liposome and astaxanthin, which is applied in application, climate change adaptation, and additional food elements, can solve the problems of low liposome encapsulation efficiency, poor dispersion stability, capture targeting, etc., to reduce The effect of high sensitivity, high stability and high bioavailability

Inactive Publication Date: 2019-12-13
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the liposomes prepared by this method have relatively low encapsulation efficiency (the encapsulation efficiency is only 65% ​​to 76%), poor dispersion stability (easy to agglomerate, precipitate), and are easily captured and targeted by the monocyte phagocytic system. lack of sex

Method used

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  • Astaxanthin nanoliposome and preparation method and application thereof
  • Astaxanthin nanoliposome and preparation method and application thereof
  • Astaxanthin nanoliposome and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1 A kind of astaxanthin nano liposome

[0048] 1, the preparation method of astaxanthin nano liposome, comprises the following steps:

[0049] (1) Accurately weigh 1.0 mg of astaxanthin, 210 mg of cholesterol and soybean lecithin (the mass ratio of the two is 1:6); completely dissolve it with a mixed solution of 5 mL of dichloromethane and chloroform at room temperature, and vortex until If there is no insoluble matter, use a rotary evaporator to remove the organic solvent in vacuum at low temperature until a uniform orange-red film is obtained on the wall of the round-bottomed flask of the rotary evaporator, and then place the round-bottomed flask in a vacuum drying oven for vacuum drying for 1 hour;

[0050] (2) Take 20 mL of pre-configured pH=7.5 phosphate buffer solution, slowly pour it into a round bottom flask, and rotate the round bottom flask at an appropriate speed, so that the substances on the inner wall of the flask are evenly dissolved in the buf...

Embodiment 2

[0081] The optimization experiment that embodiment 2 astaxanthin nano liposomes prepares

[0082] (1) Effect of Astaxanthin dosage on liposome encapsulation efficiency

[0083] On the basis of Example 1, the amount of astaxanthin added is 0.5, 1.0, 1.5, 2.0, and 2.5 mg respectively as a single factor variable, and the total amount of cholesterol and soybean lecithin is 210 mg (mass ratio is 1:6), The pH of the buffer solution was 7.5, and other conditions remained unchanged. Astaxanthin nanoliposomes were prepared according to the method of the above-mentioned Example 1, and the effect of the amount of astaxanthin added on the encapsulation efficiency of nanoliposomes was investigated.

[0084] Depend on Figure 9 It can be seen that when the amount of astaxanthin added is 1.0 mg, the encapsulation efficiency is the highest, reaching 94.12%; but when the amount of astaxanthin added is lower than 1.0 mg, the encapsulation efficiency is low; , the encapsulation efficiency show...

Embodiment 3

[0099] Effect of embodiment 3 astaxanthin nanoliposomes on the growth and immunity of Litopenaeus vannamei

[0100] (1) Test grouping and treatment

[0101] Select juvenile shrimp with normal appearance, strong constitution, and initial body weight of 0.3±0.01g, and randomly distribute them into 12 250L culture tanks, which are blank control group (group B), astaxanthin-added group (AST group), and blank control group (AST group). 4 treatment groups such as liposome group (BL group) and the astaxanthin nano-liposome group (ASTL group) of adding embodiment 1; 43d.

[0102] The prawns in group B were fed with basic feed, and the prawns in group AST, BL and ASTL were fed with basal feed containing astaxanthin, blank liposomes and astaxanthin nano-liposomes respectively; Plastids, astaxanthin and astaxanthin nano-liposomes were added to the basal feed respectively; the amount of astaxanthin added in the AST group and the ASTL group was 80 mg astaxanthin in 1 kg of feed, and the ...

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Abstract

The invention discloses an astaxanthin nanoliposome and a preparation method and application thereof. The preparation method comprises the following steps: S1, dissolving astaxanthin, cholesterol andphospholipid in a mixed solution of dichloromethane and trichloromethane, oscillating and fully mixing, decompressing and evaporating to remove an organic solvent so as to form a layer of uniform film, and then drying in vacuum; S2, adding a buffer solution into the film, eluting the film, and rotationally dissolving and uniformly dispersing to obtain an astaxanthin liposome suspension; and S3, sealing the suspension in a dark place at a temperature of 20-30 DEG C for 6-24 hours, and then carrying out membrane filtration by using a liposome extrusion instrument to obtain the astaxanthin nanoliposome. The astaxanthin nanoliposome has the characteristics of excellent characterization characteristic, high entrapment rate, uniform particle size, good dispersibility, good stability and strong antioxidant activity, greatly improves the specific growth rate and survival rate of prawns, the total antioxidant capacity and the immunity, and has the advantages of high synthesis rate, simple process and easy industrialization.

Description

technical field [0001] The invention belongs to the technical field of biomaterials. More specifically, it relates to an astaxanthin nanoliposome and its preparation method and application. Background technique [0002] Astaxanthin, the molecule is C 40 h 52 o 4 , with a melting point of about 224°C, insoluble in water, fat-soluble, and easily soluble in most organic solvents such as chloroform, acetone, ethanol, ether, etc. It belongs to the luteinoid β-carotene family and is widely distributed in nature. Natural sources of astaxanthin come primarily from organisms such as algae, yeast, salmon, trout, shrimp, lobster, and krill. Among them, Haematococcus pluvialis and Phaffia rhodozyma are the main sources of natural astaxanthin. Astaxanthin has physiological effects such as anti-oxidation, anti-aging, enhancing the body's disease resistance and coloring, and has broad application prospects in animal husbandry, aquatic products, food and drug industries. [0003] With...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K20/158A23K50/80A23K20/179
CPCA23K20/158A23K50/80A23K20/179Y02A40/818
Inventor 徐春厚刘影刘颖谢为天康恺
Owner GUANGDONG OCEAN UNIVERSITY
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