Oudemansiella canarii and application thereof

A technology of Ordinia ultifolia and Ordinia ultifolia flavonoids, which is applied in the direction of medical preparations containing active ingredients, plant raw materials, biochemical equipment and methods, etc.

Active Publication Date: 2016-07-20
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The fungus has the advantages of fast mycelial growth, short fruiting period,

Method used

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  • Oudemansiella canarii and application thereof
  • Oudemansiella canarii and application thereof
  • Oudemansiella canarii and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1. Isolation, cultivation and identification of Oddya palae JZB2115055

[0047] 1. Isolation and cultivation of Oddya palae JZB2115055

[0048] The fungus was collected from dead wood in Dadugang, Yunnan, and a comprehensive PDA medium was used (each liter was made of the following raw materials: 200g of potato, 20g of agar, 20g of glucose, 5g of soybean peptone, KH 2 PO 4 3g, MgSO 4 1.5g, vitamin B 1 10mg, the balance of water. 121 DEG C autoclaving for 30 minutes) tissue separation to obtain the pure culture (hyphae) of the bacterial strain number JZB2115055, and insert the pure culture of JZB2115055 into the enriched optimized medium (made by the following raw materials per liter: 200 g of potatoes, Glucose 20g, soybean peptone 4g, KH2 PO 4 1g, MgSO 4 0.5g, vitamin C10mg agar powder 16g, the rest of water, pH8.5) incubate at 28°C for 7-10 days at a constant temperature, transfer to the secondary seed medium, and cultivate in the dark at 24°C-26°C for 14...

Embodiment 2

[0058] Example 2. Preparation and Antioxidant Activity of Oddya palae JZB2115055 Extract

[0059] 1. Preparation of flavonoid extract from Oddya pheasantii JZB2115055

[0060] 1.1. Preparation of the flavonoid extract of Oddya halva JZB2115055

[0061] The fruiting bodies of JZB2115055 in Example 1 were dried in the air at 20-25°C to obtain dried fruiting bodies of JZB2115055. The fruiting bodies of the dried JZB2115055 are put into a high-speed universal crusher. Repeat crushing 4 times, each time for 20 seconds, to make 100 mesh uniform fruit body powder of JZB2115055. Take 10gJZB2115055 fruit body powder in a beaker, prepare 450mL of ethanol aqueous solution with a volume percentage concentration of 50% ethanol with deionized water and absolute ethanol, pour into the beaker, stir well, put it into a microwave oven, and carry out microwave intermittent radiation extraction. Intermittent microwave radiation is 3 times of microwave radiation intermittently, the first microw...

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Abstract

The invention discloses Oudemansiella canarii and application thereof. The strain number of Oudemansiella canarii is JZB2115055, and the accession serial number of Oudemansiella canarii in the China General Microbiological Culture Collection Center is CGMCC No.6171. The IC50 of the scavenging rate of Oudemansiella canarii flavone extract on DPPH free radicals is 1.73 micrograms/ml, the IC50 of the scavenging rate of Oudemansiella canarii polysaccharide extract on DPPH free radicals is 593.66 micrograms/ml, the IC50 of the total antioxidant capacity of Oudemansiella canarii flavone extract is 24.74 micrograms/ml, and the IC50 of the total antioxidant capacity of Oudemansiella canarii polysaccharide extract is 240.18 micrograms/ml. Oudemansiella canarii flavone extract and Oudemansiella canarii polysaccharide extract can be developed into an antioxidant or a free radical scavenger.

Description

technical field [0001] The invention relates to a plant of Oddya phalaenopsis and its application in the field of biology. Background technique [0002] Free radicals produced by the human body and their induced oxidation reactions are closely related to various diseases and can accelerate human aging. Antioxidants (antioxidants) can effectively scavenge free radicals, delay human aging and prevent diseases. At present, synthetic antioxidants are widely used in medical and food fields, such as BHT (di-tert-butylhydroxycresol), BHA (tert-butylhydroxyanisole), TBHQ (tert-butylhydroquinone), etc. . Studies have shown that these synthetic antioxidants have safety problems and many side effects, such as adverse effects on the human liver, spleen, and lungs, and can induce malignant tumors. Therefore, the development and utilization of biological antioxidants has become the development trend of today's food science. Most of the natural antioxidants that have been developed and ...

Claims

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Application Information

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IPC IPC(8): A61K36/07A61P39/06C12N1/14C12R1/645
CPCA61K36/07A61K2236/333A61K2236/53C12N1/145C12R2001/645
Inventor 王守现陈杰牛玉蓉赵爽刘宇许峰荣成博宋爽
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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