Quality assurance and identification method for high-quality strawberries

A technique for cultivar identification and strawberry application in biochemical equipment and methods, microbial determination/inspection, material separation, etc.

Active Publication Date: 2015-12-30
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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AI Technical Summary

Problems solved by technology

[0005] Technical Problem The purpose of this invention is to provide a quality assurance and variety identification method for high-quality strawberries, to solve the problem that existing inspectors usually use the morphological identification method and sensory evaluation commonly used in traditional agricultural production to determine the authenticity of varieties

Method used

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Embodiment Construction

[0030] The present invention will be further described below in conjunction with specific embodiment:

[0031] Xuzhou Xinxiang Strawberry Professional Cooperative Association provided 50 samples of Jiangsu's main strawberry varieties. Using HPLC-MS and GC-MS to determine the phenolic active components and volatile flavor substances of various strawberry varieties, and establish chemical fingerprints. The total antioxidant activity of each variety of strawberry was determined by ORAC. Extract DNA, design corresponding primers, measure RFLP, RAPD, SSR and PAL, CHS, F3H, DFR, ANS genes, and establish DNA fingerprints. Analyze the chemical fingerprints of different strawberry varieties and the spectrum-effect relationship of biological activity information, as well as the relationship between strawberry genetic basis and functional quality traits, establish a strawberry quality assurance evaluation system, and identify high-quality strawberry varieties.

[0032] Quality assuranc...

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Abstract

The invention relates to a quality assurance and identification method for high-quality strawberries, belonging to the technical field of biology. The quality assurance and identification method comprises the following steps: determining phenols and flavor substances of different varieties of strawberries by virtue of HPLC-MS and GC-MS, and establishing a chemical fingerprint chromatography database; determining the total antioxidant activity of the various strawberries by virtue of an ORAC method; analyzing blastogenesis diversity of the strawberries by utilizing RFLP, RAPD and SSR methods and combining key enzyme genes in synthetic routes of anthocyanin and polyphenol; establishing a spectrum-effect relationship between chemical fingerprint chromatography and biological activity information of the different varieties of strawberries, and analyzing the relation between hereditary basis and functional quality character of the strawberries. The high-quality variety of the strawberries is selected by combining chemical fingerprint chromatography, the effectiveness evaluation, genetic diversity analysis and the spectrum-effect relationship and is subjected to authenticity identification, so that a scientific, reliable, uniform and standard strawberry quality evaluation system is established, and technical support and scientific basis are provided for the quality control of the strawberries.

Description

technical field [0001] The invention relates to a method for quality assurance and variety identification of high-quality strawberries, belonging to the field of biotechnology. Background technique [0002] Strawberry (Fragaria ananassa Duch) is popular all over the world with its unique flavor and nutritional and health care value, and is one of the seven health-care fruits in the world. In recent years, China's strawberry production has developed rapidly, and it has become the world's largest producer of strawberries. The country's planting area has reached 91,200 hectares, and its output has reached 2.33 million tons, ranking first in the world. Jiangsu Province is an important strawberry producing province in my country, with a strawberry planting area of ​​about 8,300 hectares and an output of 206,000 tons. [0003] Strawberries are superfruits. Strawberries are rich in active functional ingredients such as phenolic acids, polyphenols, anthocyanins, and flavonoids. Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N21/64C12Q1/68
Inventor 黄午阳闫征李春阳王兴娜王帆
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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