Preparation and application methods of penaeus monodon fermented feed

A technology for fermenting feed and white shrimp is applied in the field of preparation of fermented feed for Penaeus vannamei, achieving the effects of good water quality environment, promoting reproduction and growth, and improving survival rate

Inactive Publication Date: 2017-09-01
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to control the raging diseases, antibiotics have been used in large quantities. Although the effect was obvious at first, the long-term and uncontrolled abuse of antibiotics has brought a series of problems to animals, farmed products and the environment.

Method used

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  • Preparation and application methods of penaeus monodon fermented feed
  • Preparation and application methods of penaeus monodon fermented feed
  • Preparation and application methods of penaeus monodon fermented feed

Examples

Experimental program
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Effect test

Embodiment 1

[0023] A preparation method for fermented feed for Penaeus vannamei and a method for using the same, comprising the following steps:

[0024] (1) Bacillus licheniformis, Lactobacillus plantarum, and Candida utilis were respectively expanded and cultured, and then mixed according to the volume ratio of 1:1:1, brown sugar (2g / 1000mL) and tap water were added to the mixed bacteria solution, and the strain solution was mixed with The ratio of water is 1:80, mixed evenly, mixed with prawn compound feed, according to the ratio of 2:5, stirred evenly, plastic film sealed and fermented for 22 hours, and the fermentation temperature is 28°C to make fermented feed.

[0025] (2) Feeding the fermented feed made to Penaeus vannamei once a day, the feeding amount is 20-30% of the total daily feeding amount, and the survival rate of prawn cultivation is increased by 15.62%, as figure 1 shown.

Embodiment 2

[0027] A preparation method for fermented feed for Penaeus vannamei and a method for using the same, comprising the following steps:

[0028] (1) Bacillus licheniformis, Lactobacillus plantarum, and Candida utilis were respectively expanded and cultured, and then mixed according to the volume ratio of 1:1:1, brown sugar (2g / 1000mL) and tap water were added to the mixed bacterial solution, and the bacterial seed solution was mixed with The ratio of water is 1:80, mixed evenly, mixed with prawn compound feed, according to the ratio of 2:5, stirred evenly, plastic film sealed and fermented for 24 hours, and the fermentation temperature is 30°C to make fermented feed.

[0029] (2) Feeding the fermented feed made to Penaeus vannamei once a day, the feeding amount is 20-30% of the total daily feeding amount, and the survival rate of prawn cultivation is increased by 16.15%, as figure 2 shown.

Embodiment 3

[0031] A preparation method for fermented feed for Penaeus vannamei and a method for using the same, comprising the following steps:

[0032] (1) Bacillus licheniformis, Lactobacillus plantarum, and Candida utilis were respectively expanded and cultured, and then mixed according to the volume ratio of 1:1:1, brown sugar (2g / 1000mL) and tap water were added to the mixed bacterial solution, and the bacterial seed solution was mixed with The ratio of water is 1:80, mixed evenly, mixed with prawn compound feed, according to the ratio of 2:5, stirred evenly, plastic film sealed and fermented for 20 hours, and the fermentation temperature is 30°C to make fermented feed.

[0033] (2) Feeding the fermented feed made to Penaeus vannamei once a day, the feeding amount is 20-30% of the total daily feeding amount, and the survival rate of prawn cultivation is increased by 16.07%, as image 3 shown.

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Abstract

The invention relates to preparation and application methods of penaeus monodon fermented feed. The preparation method comprises the following steps: respectively carrying out enlarged culture on bacillus licheniformis, lactobacillus plantarum and candida utilis, then mixing in a volume ratio of 1:1:1, adding brown sugar (2g/1000mL) and running water into the mixed bacterial solution under the condition that the ratio of the culture solution to water is 1:80, uniformly mixing, uniformly stirring with penaeus monodon compound feed in a ratio of 2:5, sealing with a plastic film, and fermenting for 18-28 hours, so that the fermented feed is obtained. The invention also provides the application method of the fermented feed. The prepared fermented feed is used for feeding penaeus monodon once a day, and the feeding amount is 20-30% of the daily total feeding amount. The prepared fermented feed can obviously improve nitric oxide synthase activity, superoxide dismutase activity, catalase activity, phenoloxidase activity, glutathione peroxidase activity and total antioxidant capacity of penaeus monodon and obviously reduce the malondialdehyde content; and composition of florae in intestines of the penaeus monodon is obviously improved, and the survival rate of penaeus monodon culture is obviously increased.

Description

technical field [0001] The invention belongs to the technical field of feed processing, and in particular relates to the preparation and application method of fermented feed for Penaeus vannamei. Background technique [0002] Fermented feed is a biologically fermented feed that uses microorganisms as biological feed starter strains to convert feed materials into microbial cell protein, biologically active small peptide amino acids, and microbially active probiotics. [0003] It has been more than 30 years since the introduction of Litopenaeus vannamei to China in the late 1980s. With the advancement of science and technology, the breeding density and intensification are getting higher and higher. The output of the product has promoted the development of the aquaculture industry; on the other hand, it has also brought about a serious problem that cannot be ignored-the deterioration of the aquaculture environment, coupled with the degradation of the excellent germplasm of praw...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/80A23K10/18
Inventor 崔青曼吴佩佩孟阳袁春营
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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