Root tip detoxification and rapid propagation technology for purple potatoes

A purple potato and potato technology, which is applied in the field of root tip detoxification and tissue culture rapid propagation of purple potato, can solve the problems of high pollution rate of tissue culture seedlings, difficult and thorough disinfection, and complicated operation, and achieves thorough disinfection and test-tube seedling propagation. The effect of speed and mini-tuber yield improvement and potato yield improvement

Inactive Publication Date: 2010-10-06
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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Problems solved by technology

[0008] The purpose of the present invention is to propose a simple operation, high efficiency, easy and thorough disinfection method for the problems of cumbersome operation, low efficiency, difficult and thorough disinfection and high pollution rate of tissue cultured seedlings in the tr

Method used

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  • Root tip detoxification and rapid propagation technology for purple potatoes
  • Root tip detoxification and rapid propagation technology for purple potatoes
  • Root tip detoxification and rapid propagation technology for purple potatoes

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1: (a kind of purple potato root tip detoxification and rapid propagation technology 1)

[0048] (1) The preparation of the medium, including the basic medium and the components of the medium at each stage and the weight per liter are:

[0049] 1) Basic medium: Potato basic medium + NH 4 NO 3 400mg / L+KNO 3 50mg / L+KH 2 PO 4 500mg / L+sucrose 25g / L+agar 7g / L, pH 5.8;

[0050] 2) Root tip induction medium: potato basal medium + NH 4 NO 3 400mg / L+KNO 3 500mg / L+KH 2 PO 4 50mg / L+BA0.5mg / L+NAA0.7mg / L+hydrolyzed milk protein 1g / L+sucrose 20g / L;

[0051] 3) Proliferation medium: Potato basal medium + NH 4 NO 3 400mg / L+KNO 3 500mg / L+KH 2 PO 4 50mg / L+BA0.5mg / L+NAA0.2mg / L+hydrolyzed milk protein 1g / L+sucrose 20g / L;

[0052] 4) Subculture growth medium: Potato basal medium + NH 4 NO 3 400mg / L+KNO 3 500mg / L+KH 2 PO 4 50mg / 0.5L+BA0.2mg / L+NAA0.1mg / L+hydrolyzed milk protein 1g / L+sucrose 20g / L;

[0053] 5) Rooting medium: Potato basic medium + NH 4 NO 3 4...

Embodiment 2

[0071] Embodiment 2: (a kind of purple potato root tip detoxification and rapid propagation technology 2)

[0072] (1) The preparation of the medium, including the basic medium and the components of the medium at each stage and the weight per liter are:

[0073] 1) Basic medium: Potato basic medium + NH 4 NO 3 400mg / L+KNO 3 50mg / L KH 2 PO 4 500mg / L+ sucrose 25g / L+ agar 8g / L, pH 5.7;

[0074] 2) Root tip induction medium: potato basal medium + NH 4 NO 3 400mg / L+KNO 3 500mg / L+KH 2 PO 4 50mg / L+BA0.8mg / L+NAA0.5mg / L+hydrolyzed milk protein 1g / L+sucrose 20g / L;

[0075] 3) Proliferation medium: Potato basal medium + NH 4 NO 3 400mg / L+KNO 3 500mg / L+KH 2 PO 4 50mg / L+BA0.35mg / L+NAA0.35mg / L+hydrolyzed milk protein 1g / L+sucrose 20g / L;

[0076] 4) Subculture growth medium: Potato basal medium + NH 4 NO 3 400mg / L+KNO 3 500mg / L+KH 2 PO 4 50mg / 0.5L+BA0.35mg / L+NAA0.1mg / L+hydrolyzed milk protein 1g / L+sucrose 20g / L;

[0077] 5) Rooting medium: Potato basic medium + NH 4 NO...

Embodiment 3

[0081] Embodiment 3: (a kind of purple potato root tip detoxification and rapid propagation technology 3)

[0082] (1) The preparation of the medium, including the basic medium and the components of the medium at each stage and the weight per liter are:

[0083] 1) Basic medium: Potato basic medium + NH 4 NO 3 400mg / L+KNO 3 50mg / L KH 2 PO 4 500mg / L+sucrose 25g / L+agar 9g / L, pH 5.6;

[0084] 2) Root tip induction medium: potato basal medium + NH 4 NO 3 400mg / L+KNO 3 500mg / L+KH 2 PO 4 50mg / L+BA1.0mg / L+NAA0.3mg / L+hydrolyzed milk protein 1g / L+sucrose 20g / L;

[0085] 3) Proliferation medium: Potato basal medium + NH 4 NO 3 400mg / L+KNO 3 500mg / L+KH 2 PO 4 50mg / L+BA0.2mg / L+NAA0.5mg / L+hydrolyzed milk protein 1g / L+sucrose 20g / L;

[0086] 4) Subculture growth medium: Potato basal medium + NH 4 NO 3 400mg / L+KNO 3 500mg / L+KH 2 PO 4 50mg / 0.5L+BA0.5mg / L+NAA0.1mg / L+hydrolyzed milk protein 1g / L+sucrose 20g / L;

[0087] 5) Rooting medium: Potato basic medium + NH 4 NO 3 4...

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Abstract

The invention discloses root tip detoxification and rapid propagation technology for purple potatoes and belongs to the technical field of plant detoxification and tissue culture rapid propagation. The method comprises the following steps of: (1) preparing a culture medium; (2) performing root tip detoxification and rapid propagation; and (3) performing multi-reverse transcription-polymerase chain reaction (RT-PCR) synchronous and rapid detection on complex virus infection of a purple potato test tube plantlet or a test tube mini-tuber and the like. In the technology, each level of culture medium is adjusted according to the characteristic of the purple potato and conventional stem tip detoxification is changed into root tip detoxification so as to simplify operation process, avoid high pollution rate and improve working efficiency. Moreover, the technology has the advantages of good inoculant differentiation, high test tube plantlet propagation speed, complete detoxification, high mini-tuber growth vigor, monthly mini-tuber reproduction rate of 4 to 6 times and mini-tuber rooting rate and transplanting survival rate of over 98 percent. The technology can be popularized in regions which are suitable for the growth of purple potatoes.

Description

technical field [0001] The invention relates to the technical field of plant detoxification and tissue culture rapid propagation, in particular to a purple potato root tip detoxification and tissue culture rapid propagation technology. Background technique [0002] Purple potato is an annual crop of Solanaceae, native to Australia. Potato-shaped long oval, smooth skin, purple-black potato flesh, rich in anthocyanins, Vc, carotene, etc., with edible and medicinal functions. Anthocyanins are water-soluble pigments that exist in plants and belong to flavonoid compounds. They are the most direct, effective and safe free radical scavenger found in the scientific community to prevent and treat diseases and maintain human health. Its ability to scavenge free radicals It is 20 times of VC and 50 times of VE. Anthocyanins have a small molecular structure, and are the only substances that can clear free radicals and protect brain cells through the blood-brain barrier, and at the sam...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 严成其陈剑平余初浪王栩鸣杨勇蒋益虹羊健徐刚程晔王芳刘键朱红芬沈岚黄坚
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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