Screening crop anti reversibility strain

A technology of strains and crops, applied in botany equipment and methods, plant genetic improvement, application, etc., can solve problems such as high cost, long screening cycle, complicated operation, etc., and achieve the effect of reliable data, reduced planting cost, and simple operation

Inactive Publication Date: 2009-03-18
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a method for screening crop stress-resistant strains in order to solve the problems of long screening period, high cost, complicated operation and various uncertain factors in the existing methods for screening and cultivating stress-resistant strains

Method used

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  • Screening crop anti reversibility strain
  • Screening crop anti reversibility strain
  • Screening crop anti reversibility strain

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Experimental program
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specific Embodiment approach 1

[0010] Specific Embodiment 1: The method for screening crop stress-resistant strains in this embodiment is realized by the following steps: 1. Dissolve NaCl or PEG in water in different proportions to make solutions with different mass concentrations; Carry out routine germination test in the solution of different mass concentrations in the step 1; 3, after germination 3~4 days, select the solution that the seed germination rate is 4%~6% as the solution for screening stress-resistant strains; 4, soak the seeds in screening stress-resistant strain A conventional germination test is carried out in the solution, and the germinated seeds obtained are stress-resistant strains.

[0011] Because of the difference in plant species or varieties, the screening stress-resistant strain solution of its seeds is also different (the concentration of solute in the screening stress-resistant strain solution is different), so in the process of screening stress-resistant strains, it is necessary ...

specific Embodiment approach 2

[0016] Embodiment 2: This embodiment differs from Embodiment 1 in that the PEG in step 1 is PEG6000. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0017] Embodiment 3: This embodiment is different from Embodiment 1 in that the seeds in step 2 are field crop seeds, garden crop seeds or pasture seeds. Other steps and parameters are the same as those in Embodiment 1.

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Abstract

The present invention relates to a method for filtering crop stress-resistant strain and relates to a method for filtering crop strain. The method solves the problems of the existing method for filtering and cultivating the stress-resistant strain of long filtering period, high cost, complex operation and various uncertain factors. In the method, seeds are dipped in aqueous solution which uses NaCl or PEG as a solute and has different mass concentrations; the seeds germinate generally; then the solution concentration with the rate of emergency being 4 percent to 6 percent is selected as the concentration for filtering the stress-resistant strain; a seed young seedling is prepared firstly; the seed young seedling is dipped in the aqueous solution which uses NaCl or PEG as the solute and has different mass concentrations for stress treatment and cleaned; then the seed young seedling is dipped in distilled water to be cultivated; and the solution mass concentration with the seed young seedling root tip regenerating rate being 4 percent to 6 percent is selected as the concentration for filtering the stress-resistant strain. The height and the stem diameter of a plant obtained by the method are larger than the height and the stem diameter of unfiltered plants, and the method reduces the growth period greatly, has simple operation and reliable data and reduces the planting cost by 20 percent to 50 percent.

Description

technical field [0001] The present invention relates to methods of screening crop lines. Background technique [0002] In recent years, with the warming of the global climate, the degree of aridity and salinization of cultivated land is increasing and strengthening, which makes agricultural production face extremely severe tests. At present, in order to deal with the threat of drought and salinity to agriculture, there are three relatively effective countermeasures: 1. Use the existing germplasm resources to carry out recurrent breeding in the field, but this method is time-consuming and laborious due to the complex field environmental conditions , so that the results lack reliability; second, the use of hybridization methods requires a long cycle and high cost; third, the use of genetic engineering, this method also has the problem of a long cycle; All of them have the problems of long screening period, high cost, complicated operation and various uncertain factors. Cont...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/04
Inventor 周道玮田雨肖模昕管博
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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