Dendrobium officinale rapid breeding method through root tip tissue culture

A technology of dendrobium candidum and root tip is applied in the field of rapid propagation of dendrobium candidum root tip tissue culture to achieve the effects of short culture time, good resistance and maintaining growth characteristics

Active Publication Date: 2015-08-12
广西正能农林健康产业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Modern artificially planted seedlings come from the sexual reproduction of Dendrobium officinale seeds. Due to the natural breeding conditions, the offspring of seed reproduction have more variation, individual plant differences are large, the characters are unstable, the production cost is high, and the yield is low. Therefore, the fine varieties Seed saving and multiplication has become an urgent need

Method used

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Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0018] Select robust Dendrobium officinale seedlings, take about 1.5cm root tip and wash it with running water for about 1 hour, then disinfect it with 75% ethanol solution for 40 seconds, then rinse it twice with sterile water, then put the material into a beaker and wash it with 0.1% ethanol solution. Soak in mercuric chloride for 8 minutes, then rinse with sterile water 4-5 times, take 0.3-0.5mm root tips and inoculate them into differentiation induction medium: MS medium (100 parts) + NAA0.4mg / L (3.5 parts ) + banana juice 10% (6.4 parts) + agar 5.7g / L (3.7 parts) + sucrose 30g / L (28 parts), at a temperature of 25°C, a light intensity of 1800lx, and a light time of 12 hours, cultivate two moon. Post-transplant proliferation medium: MS medium (100 parts) + NAA1.0mg / L (1.5 parts) + 6-BA0.2 mg / L (1.2 parts) + potato juice 10% (7.3 parts) + agar 5.7 g / L (1.8 parts) + 30 g / L sucrose (14.3 parts), cultivated for one and a half months at a temperature of 25°C, a light intensity ...

Embodiment 2

[0020] Disinfection of root tips: Select robust Dendrobium officinale seedlings, take 1~1.5cm root tips, wash them with running water for 1 hour, sterilize with 75% ethanol solution for 40 seconds, rinse them with sterile water twice, put the root tips into the container, and use Soak in 0.1% mercuric chloride for 8 minutes, then rinse with sterile water 4-5 times to obtain the root tip to be inoculated;

[0021] Root tip differentiation: Cut out 0.3-0.5 mm of the root tip to be inoculated, insert it into the induction differentiation medium, and cultivate it for 45 days under the conditions of temperature 25°C, light intensity 1800 lx, and daily light time 12 hours to obtain differentiation root tip; the induction differentiation medium is 100 parts of MS medium, 5.5 parts of 0.4mg / L NAA, mass fraction is 1-10 parts of 10% banana juice, 0.5-5 parts of 5.7g / L agar, 30g / L A mixture of 18 parts of sucrose, 12.5 parts of pine pollen, and 6 parts of diatom powder.

[0022] Proli...

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PUM

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Abstract

The invention discloses a dendrobium officinale rapid breeding method through root tip tissue culture. The breeding method comprises the following steps: subjecting the root tip to induced differentiation, culturing the root tip, performing proliferation culture, carrying out rooting culture to obtain rooted seedlings, and finally performing acclimatization and transplanting to obtain finished seedlings. The cultured dendrobium officinale seedlings inherit the excellent characters of artificially-selected plants, the resistance is good, the yield is stable, and thus the seedlings are worthy to promote.

Description

[0001] Technical field: [0002] The invention relates to a seedling raising method for root tip tissue culture rapid propagation of dendrobium officinale, belonging to the technical field of bioengineering. [0003] Background technique: [0004] Wild Dendrobium candidum is listed as an endangered species by the state due to its harsh growth conditions and extremely rare output. Modern artificially planted seedlings come from the sexual reproduction of Dendrobium officinale seeds. Due to the natural breeding conditions, the offspring of seed reproduction have more variation, individual plant differences are large, the characters are unstable, the production cost is high, and the yield is low. Therefore, the fine varieties Seed conservation and multiplication has become an urgent need. The invention adopts manual selection of excellent plants, and through the rapid propagation technology of virus-free seedling tissue culture, the offspring of the seedlings inherit the e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 刘开红
Owner 广西正能农林健康产业有限公司
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