Method for unconventionally breeding improved cows through embryo biotechnology

A biotechnology and dairy cow technology, applied in the field of bioengineering, can solve the problems that restrict the development speed of dairy cows, and achieve the effects of ultra-rapid breeding, lower production costs, and control birth rate

Inactive Publication Date: 2014-09-17
NANTONG HUIRAN BIOLOGICAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Especially in superovulation, in vitro fertilization, embryo collection, gender identification, embryo segmentation, embryo cloning, embryo cryopreservation and embryo transfer, there have been great breakthroughs and progress, which has accelerated the reproduction and development of dairy cows to a certain extent. Quality improvement, but in terms of controlling the birth sex of dairy cows, there is no breakthrough, which will seriously restrict the development speed of dairy cows' super-breeding

Method used

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Embodiment Construction

[0024] A method for super-conventional breeding of fine-bred dairy cows using embryonic biotechnology, selecting excellent and suitable cows as donors, said method comprising the following steps: a, superovulation: in the first 4 days of estrus of the selected cows, i.e. the estrus cycle On the 16th or 17th day, inject PMSG1500-3000IU intramuscularly or subcutaneously; b. Artificial insemination: After the superovulation-treated cows are in estrus, select the semen of good bulls and perform artificial insemination at an appropriate time; c. Embryo flushing: use the two-way catheter egg flushing device to inject the flushing liquid into the uterine horn through the inner tube, then export the flushing liquid, and obtain the required embryo flushing liquid; d. Embryo inspection: wash the collected embryos in step c liquid, let it stand for 10min, after the embryos sink, remove the supernatant, and put them under a microscope to check the quantity and development; e, embryo freezi...

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Abstract

The invention relates to a method for unconventionally breeding improved cows through an embryo biotechnology. Excellent and suitable female cows are selected as donors. The method comprises the following steps of (1) superovulation, (2) artificial insemination, (3) embryo washing, (4) embryo checking, (5) embryo freezing, (6) embryo unfreezing, (7) embryo separation, and (8) embryo transplantation. By the adoption of the method, the birth rate of female calves can be effectively controlled, production cost is reduced, quality of the cows and milk is improved, and the purpose of unconventionally and rapidly breeding the cows is achieved.

Description

technical field [0001] The invention relates to a bioengineering technology, in particular to a method for superconventional breeding of fine-bred dairy cows using embryonic biotechnology. Background technique [0002] As one of the most comprehensive and complete foods with nutrition, milk has always been valued by people. Especially with the improvement of people's living standards and the continuous improvement of dietary structure, the social demand for milk continues to increase. According to the conventional breeding and development speed of dairy cows, it is difficult to meet people's growing demand for milk. How to utilize high and new technology to breed dairy cows is an important and urgent task before the vast number of scientific and technological workers, especially animal husbandry workers. [0003] my country began to study embryo bioengineering technology in the 1970s, and the research and development speed accelerated after the 1980s. In recent years, grati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D19/00
Inventor 不公告发明人
Owner NANTONG HUIRAN BIOLOGICAL SCI & TECH
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