38 results about "Sample stability" patented technology

The application discloses a hydrate sediment sample preparation device and method at different stratum depths, and a sample stability-maintaining transfer method. The device comprises a sample preparation kettle placed in a constant-temperature water bath box, an axial pressure loading assembly connected with the sample preparation kettle and used for applying axial pressure load to the sediment sample, and a pore pressure generating assembly connected with a cavity in the sample preparation kettle. The preparation process comprises the following steps: preparing the sediment in the sample container; transferring the sediment sample to a centrifuge; adding axial pressure consolidation; injecting high-pressure methane gas; preparing the sample under supergravity; stopping the centrifuge and moving to liquid nitrogen; sampling under normal gravity while maintaining stability; and transferring the sample while maintaining stability. The application can simulate the preparation of hydrate-containing sediment samples under effective stress levels at different stratum depths in a laboratory supergravity environment, and can transfer the samples to a scanning electron microscope or other microscopic observation device for testing while maintaining the stability of the hydrates.

The present disclosure provides icIEF sample matrices that enable icIEF analysis of PEGylated proteins in their real conjugated states. The sample matrices of the present disclosure can include glycine, which enables the separation of co-migrated PEGylated protein charge variants. The sample matrices can also include taurine, which further improves icIEF assay by depleting matrix induced baseline interferences. Accordingly, a sample matrix of the present disclosure including a combination of glycine and taurine enables icIEF separation of acidic and basic species from the main peak for PEGylated proteins, allowing the identification / separation, characterization and quantification of discrete PEGylated protein species. By using the sample matrices of the present disclosure to characterize PEGylated proteins by icIEF, repeatability, linearity, accuracy, sample stability, and method robustness are achieved.

The invention discloses a hot stamping film coating adhesive force testing device which comprises a testing table, a transverse sliding table module is arranged at one end of the upper portion of the testing table in the horizontal direction, and a height sliding table module is arranged at the position, close to one end of the transverse sliding table module, of the testing table in the vertical direction. The transverse sliding table module is slidably provided with a sample table for placing a sample, the height sliding table module is slidably provided with a sensor assembly for fixing a to-be-tested film layer on the sample, a chip processor is arranged in the test board, and the transverse sliding table module, the height sliding table module and the sensor assembly are electrically connected with the chip processor. By specially designing the sample table for fixing the hot stamping film sample, the stability of the sample is remarkably enhanced during testing, the measurement error is effectively reduced, flexible adjustment within the range of 90-180 degrees can be realized according to material characteristics and use requirements, the test condition is ensured to be consistent with the actual application environment, and the test efficiency is improved. And the accuracy and practicability of the test result are further improved.

The invention relates to the technical field of sample preparation, and discloses a sample preparation method for microscopic characterization of a sample profile. The method comprises the steps of sample pretreatment, specific position positioning, profile preparation, sample separation, carrier transfer and fixation and the like, cutting parameters are accurately controlled through a focused ion beam (FIB), and a silicon wafer carrier and Pt / C / W welding and fixing technology is combined. The problems that in an existing sample preparation method, sample preparation precision is low, conductivity is poor, fixation is unstable, and multi-characterization combination adaptability is insufficient are solved. Nondestructive preparation of a small-size micro-area profile at a specific position is realized, the sample stability is high, various characterization means such as nano infrared and nano SIMS are compatible, the accuracy and relevance of analysis data are ensured, and the method is suitable for microscopic characterization requirements in the fields of semiconductor integrated circuits, nano coatings, biomedical materials and the like.

The invention belongs to the technical field of ultra-deep and ultra-high pressure ground sample transfer, and relates to an ultra-deep and ultra-high pressure condensate gas well sampling and transferring device and a use method thereof. Comprising a sampler, the sampler is used for completing sampling from the ultra-deep ultrahigh pressure condensate gas well, and a sample transferring container is used for transferring a sample. The sample transfer container is filled with alcohol substances, and when a sample is transferred into the sample transfer container, the alcohol substances are synchronously discharged to the liquid collection container, so that the stability of the pressure in the sample transfer container is ensured, and the stability of the sample in the sample transfer process is ensured. A switch unit is arranged on the communicating pipeline and is used for controlling communication and disconnection between the sampler and the sample transfer container. And the first pressure measuring and regulating part is used for regulating the pressure in the sampler, so that stable sample transfer of the sample is facilitated. And the second pressure measuring and regulating part is used for regulating the pressure in the sample transferring container to ensure the stability of the sample transferring process. According to the invention, the sample transfer in the ultra-deep ultrahigh pressure condensate gas well sampler can be realized, and the sample transfer process is safe and reliable.

The invention provides a Raman characterization method based on targeted modification of SHINERS and co-pool synchronous monitoring of heterogeneous Fenton-like reaction, and belongs to the technical field of cross of spectral analysis and environmental catalysis. Aiming at the defects of poor sample preparation stability, large measurement system error and only qualitative data analysis in the prior art, through innovative fusion of a targeted stable sample preparation technology and a dual-electrode common-pool synchronous monitoring system, a time-resolved in-situ Raman spectrum and a targeted modified shell layer isolated nanoparticle enhanced Raman spectrum are combined for use; dynamic in-situ characterization of transient intermediates, active species and reaction paths on the surface of the catalyst in a heterogeneous Fenton-like reaction system is realized. The method can adapt to various catalysts and oxidants, qualitative characterization and process detection of heterogeneous reaction are achieved, breakthrough technical support is provided for analysis of a Fenton-like reaction mechanism, and the problems that a traditional sample preparation and measurement method is poor in universality and insufficient in uniqueness are solved.

The invention relates to a sample treatment liquid for respiratory virus antigen detection and a preparation method thereof. The sample treatment liquid comprises a phosphate buffer solution, 0.05-0.5 g / L of sodium caseinate, 1-20 g / L of bovine serum albumin, 10-50 mL / L of a surfactant, 1-10 g / L of guanidine hydrochloride, 3.72-37.2 g / L of an ethylene diamine tetraacetic acid disodium salt diaqueous solution, 1-5 mL / L of a CL preservative, 1-5 mL / L of a BND bacteriostatic agent and 900-988 mL / L of sterile deionized water. The method has the advantage of good sample stability.

The utility model discloses an intertidal zone surface sediment sampler which comprises a supporting plate, a vertical pipe is fixedly connected to the top of the supporting plate, scale marks are arranged at the bottom of the front side of the vertical pipe, a handle rod is fixedly connected to the top end of the vertical pipe, and a rotary supporting assembly is fixedly connected to the bottom of the supporting plate. The two sides of the rotary supporting assembly are fixedly connected with triangular shovel boxes with the bottoms of conical structures, and the two triangular shovel boxes are symmetrically arranged. According to the utility model, a series of structures are arranged, so that the two triangular shovel boxes can be conveniently inserted into intertidal zone surface sediments in an electric driving extrusion closing manner, and then stable shovel holding sampling is carried out, the collected surface sediments can be stably limited and prevented from leaking, and the sampling stability is improved; the sampling depth is conveniently limited, the limiting position is flexibly and accurately adjusted according to surface sampling requirements of different depths, personnel can conveniently and accurately control the sampling depth of surface sediments, and the sampling accuracy is improved.

The invention discloses a preparation method of a drift correction sample for X fluorescence analysis of a free acid-containing product in copper smelting, and belongs to the technical field of drift correction of an X fluorescence analyzer. The method comprises the following steps: (1) selecting a free acid-containing product generated in a copper smelting process, and adding the free acid-containing product into high-purity water to prepare slurry; (2) adding a saturated solution of alkali metal hydroxide or alkaline earth metal hydroxide into the slurry until the pH value is 6.8-7.2 to obtain a liquid mixture; (3) filtering the liquid mixture to obtain a solid substance; (4) drying, crushing and uniformly mixing the solid substance to obtain uniform powder; and (5) pressing the uniform powder into a circular sheet to obtain the drift correction sample. According to the method, the free acid is neutralized, so that the negative effects of corrosion of the free acid on the X fluorescence analyzer, aggravation of fracture damage, chemical component change and the like of the drift correction sample are avoided, and the drift correction accuracy and the drift correction sample stability are improved.

The utility model provides a coal detection sample transportation device, relates to the technical field of coal detection, and solves the technical problems that a coal detection sample transfer trolley is single in function and cannot shake up samples in the transfer process. The coal detection sample transporting device comprises a vehicle body, a driving device located on the vehicle body, a rotating shaft, a rotating arm and a tank, the driving device is fixed to the vehicle body, the driving device is in driving connection with the rotating shaft and used for driving the rotating shaft to rotate with the axis of the rotating shaft as the axis, the rotating shaft is hinged to the rotating arm, and the rotating arm is rotationally connected with the side wall of the tank; a containing plate is fixed in the tank body and used for fixing a sample bottle, and the tank body can be driven by the rotating arm to swing so as to shake the sample bottle; the driving device drives the rotating shaft to rotate to realize rotation of the rotating arm, so that the tank body swings to automatically shake up the sample bottle, the problems of low manual shaking-up efficiency and poor sample uniformity are solved, and the device has the advantages of improving the detection efficiency and the sample stability.

The invention provides a method for measuring the content of active ingredients in gardenia-rhubarb decoction. The method comprises the following operation steps: 1) preparing a reference solution; (2) preparing a test solution; and (3) detecting by adopting an HPLC (High Performance Liquid According to the method for determining the content of the active ingredients in the gardenia-rhubarb decoction, the reference sample of the rhubarb decoction is obtained on the basis of the original record of Jingui Litu and is used for constructing the method for detecting the active ingredients in the gardenia-rhubarb decoction; according to the method, high-resolution detection of seven key functional components including genipin gentiobioside, geniposide, narirutin, hesperidin, daidzein, emoditin-7-O-beta-D-glucoside and genistein is realized, the specificity is high, the repeatability is good, a reliable technical support is provided for quality standard improvement and clinical precise medication of the gardenia-rhubarb decoction, and the method has a wide application prospect. The method has important significance on guaranteeing the stability of the gardenia-rhubarb decoction reference sample.

This invention relates to the field of internal quality control technology for nucleic acid testing, and discloses a method for preparing an internal quality control sample for viral nucleic acid testing for blood screening. The method includes: mixing HCV RNA-positive plasma; mixing the HCV RNA-positive plasma with HCV RNA-negative plasma or newborn calf serum to obtain a first plasma mixture; mixing the plasma mixture with methylene blue solution to obtain a second plasma mixture; and irradiating the second plasma mixture with light. The method for preparing an internal quality control sample for viral nucleic acid testing for blood screening provided by this invention yields a sample with good stability and low cost.

The utility model application relates to the field of biological experiment equipment discloses a kind of living cell tissue perfusion collection systems.The system includes: liquid flow switching dosing module, constant temperature incubation module, sample automatic collection module and sample low-temperature maintenance module.Liquid flow switching dosing module switches different liquid according to experimental requirement anytime, average shunt pumps into constant temperature incubation module;Constant temperature incubation module provides closed, constant temperature incubation liquid flow environment for living cell tissue perfusion experiment;Sample automatic collection module receives the perfusion liquid sample flowing through constant temperature incubation module, and according to the specified time sequence, sample is collected into collection well plate;Sample low-temperature maintenance module keeps sample collection plate in relatively low temperature state by refrigeration temperature control mode, to delay sample volatilization and the denaturation of the substance to be tested in it, guarantee sample stability.Through the system, perfusion liquid sample can be accurately collected according to time sequence, improve experimental result accuracy, to obtain repeatable high-quality experimental result.

The invention provides an outer vesicle surface protein in-situ labeling method, and belongs to the technical field of outer vesicles, and the outer vesicle surface protein in-situ labeling method provided by the invention comprises the following steps: carrying out differential centrifugal separation on outer vesicles, carrying out quality evaluation by adopting nano-particle tracking analysis, and establishing a positive and negative correlation coupling equation set to optimize labeling conditions. The method comprises the following steps: fixing a sample by using glutaraldehyde, labeling antibodies in an optimized labeling buffer solution, removing free antibodies through ultrafiltration centrifugation, carrying out singular value decomposition calculation, adding a special protective agent, analyzing morphological characteristics by adopting a deep learning model, and ensuring the stability of the sample through filter membrane filtration and low-temperature preservation. In the whole process, a complete quality control system is established, the labeling targets of high specificity, high efficiency and high stability are achieved, and the technical problems of low labeling specificity and poor stability in the in-situ labeling process of the outer vesicle surface protein in the prior art are solved.

The invention relates to the technical field of production of grid samples for electron microscopes, and discloses a grid sample production device for electron microscopes, which comprises a main body assembly and a frame body, a first air cylinder is fixed on the frame body, a tweezers adapter is fixed at the output end of the first air cylinder, and tweezers are arranged below the tweezers adapter. Grids are clamped by the tweezers, and the tweezers adapter is connected with the tweezers through a connecting piece. And the freezing assembly is arranged in the frame body and comprises a freezing piece. The device has the beneficial effects that the connecting piece cooperates with multiple springs through a limiting block and a swing rod, quick disassembly and assembly of tweezers and an adapter are achieved, the direction of the tweezers can be positioned, grid angle deviation is avoided, the operation efficiency and the sample stability are improved, a moving piece, a limiting piece and a screening piece in the freezing assembly are linked, an empty storage barrel is automatically searched, freezing grids are stored in a sealed mode, and the freezing efficiency is improved. A low-temperature environment is guaranteed and storage efficiency and sample quality are improved.

1. The name of this design product: Low-speed refrigerated positioning centrifuge. 2. The use of this design product: For the low-temperature high-speed separation and extraction of biological and chemical samples to maintain sample stability. 3. The design key points of this design product: Lies in the shape. 4. The picture or photo that best shows the design key points: Perspective view.

The embodiment of the invention discloses a seawater pH quality control sample and a preparation method thereof, and belongs to the technical field of analysis and detection. The method comprises the following steps: dissolving a buffer reagent in simulated seawater, and adjusting the pH value to a target value by using hydrochloric acid or sodium hydroxide to prepare a seawater pH quality control sample with the pH range of 2-10. The quality control sample with the pH range of 2-10 can be obtained, the wide range from strong acidity to weak alkalinity is covered, the pH measurement requirements of seawater samples in different sea areas and different depths are met, the pH quality control sample is good in stability, the validity period is as long as 2 years, the pH quality control sample can be widely applied to the fields of marine scientific research, environmental monitoring, water quality analysis and the like, and the application prospect is wide. And reliable technical support is provided for accurate measurement and quality control of the pH value of seawater.

This application discloses an excitation device belonging to the field of oil detection technology. The excitation device is configured with a sample feeding unit, a sample delivery unit, a rotation unit, and a sampling unit. The second driving component in the sample delivery unit ensures high accuracy in positioning of the rod electrode and can stably generate an electric arc with the disk electrode. The third driving component in the sampling unit ensures that the disk electrode can be stably immersed in the sample oil, guaranteeing sampling stability. After one sampling excitation is completed, the first driving component in the sample delivery unit maintains the distance between the rod electrode and the disk electrode at a preset distance, eliminating the need to replace the rod electrode or manually adjust the distance between them. Driven by the first driving component, the next sampling excitation can be performed quickly, improving excitation efficiency and thus enhancing the detection efficiency of the arc direct-reading atomic oil spectrometer. Moreover, the entire process requires no manual operation, has a high degree of automation, and ensures safety during use.

The invention discloses a high performance liquid chromatography detection method for naphthalene in medium / long-chain fat emulsion injection (C8-24Ve). According to the method, the naphthalene in the medium / long-chain fat emulsion injection (C8-24Ve) can be detected in a short time by utilizing a high performance liquid chromatograph. The method is simple, convenient to operate, efficient and rapid, the most common chromatographic column and detector are adopted, naphthalene detection in the medium / long-chain fat emulsion injection (C8-24Ve) is achieved within 35 min, the selected mobile phase cannot damage an instrument, the detection time is short, and the method is an efficient and reasonable analysis method. The sample stability is good, the product quality is ensured, the production and daily detection of enterprises are facilitated, the efficiency is improved, and the detection cost is greatly reduced.

The application relates to a sample processing liquid for respiratory virus antigen detection and a preparation method thereof, which comprises a phosphate buffer, 0.05-0.5 g / L sodium caseinate, 1-20 g / L bovine serum albumin, 10-50 mL / L surfactant, 1-10 g / L guanidine hydrochloride, 3.72-37.2 g / L ethylenediaminetetraacetic acid disodium salt dihydrate solution, 1-5 mL / L CL preservative, 1-5 mL / L BND bacteriostatic agent and 900-988 mL / L sterile deionized water; and the application has the advantages of good sample stability.

The invention relates to the technical field of traditional Chinese medicine analysis, in particular to a construction method of a Yuquan capsule characteristic spectrum. Comprising the following steps: 1, preparing a reference substance solution; 2, preparing a test solution; 3, determining conditions; and 4, generating a contrast characteristic spectrum. According to the invention, methodological investigation is carried out on the constructed Yuquan capsule characteristic spectrum method, and the methodological investigation comprises an instrument precision experiment, a method repeatability experiment and a sample stability experiment. In each experiment result, the RSD value of the relative retention time of each peak is less than or equal to 1.0%, and the RSD value of the relative peak area is less than or equal to 10.0%. By means of the method, large types of chemical components in the Yuquan capsules can be reflected, and the overall quality of the Yuquan capsules can be controlled more efficiently and rapidly.

The invention relates to a method for determining eight components, namely gastrodin, chlorogenic acid, genipin gentiobioside, geniposide, albiflorin, paeoniflorin, 2, 3, 4, 5-tetrahydroxy stilbene-2-O-B-D-glucoside and baicalin, in a paeonia lactiflora and ephedra spasm relieving granule extract. The methodology verifies that the precision, repeatability, sample stability and sample recovery rate of the method disclosed by the invention are good, the result meets the content determination requirements, and the method can be used for effectively determining the content of gastrodin, chlorogenic acid, genipin gentiobioside, geniposide, albiflorin, paeoniflorin, 2, 3, 4, 5, 6-tetramethyl-7-oxo-7-oxo-7-oxo-7-oxo-7-oxo-7-oxo-7-oxo-7-oxo-7-oxo-7- The composition is prepared from eight components including 1, 2, 3, 4, 5-tetrahydroxy stilbene-2-O-B-D-glucoside and baicalin.

The invention provides a proficiency testing liquid sample stability testing method and related equipment, and relates to the technical field of radionuclide analys.The method comprises the steps that a preset number of to-be-treated liquid samples are randomly extracted from liquid samples, stability testing parameters are determined, the to-be-treated liquid samples are placed in a corresponding testing environment to be treated, and the to-be-treated liquid samples are obtained; the stability test parameters at least comprise temperature, analyzing according to a standard analysis method to obtain a measured average value of the radionuclides, correcting the measured average value to a preset test date according to the half-life period of the radionuclides to obtain a corrected average value, and calculating the stability of the radionuclides according to the corrected average value. And obtaining a stability conclusion of the liquid sample under the stability test parameters. According to the technical scheme, factors influencing the stability of the proficiency testing liquid sample can be tested, the influence of the factors is avoided in the process of analyzing the activity concentration of the radionuclide, and the reliability of the analysis result of the activity concentration of the radionuclide is improved.

The invention provides a method for detecting oxalic acid in urine by adopting an HPLC-ESI-MS / MS. The method comprises the following steps: S1, taking a collected sample, adding oxalic acid-1, 2-13C as an internal standard of oxalic acid, mixing an internal standard solution and the collected sample according to a ratio of 2: 1, performing vortex for 5 minutes, and performing centrifugation for 5 minutes at 4000 rpm to obtain a urine sample; s2, detecting the sample by using a liquid chromatography tandem mass spectrometer; s3, adopting stable isotope labeled oxalic acid-1, 2-13C as an internal standard of oxalic acid, preparing a series of standard substances with different concentrations, making a standard curve, taking the concentration of an oxalic acid working standard solution as an X axis, taking a peak area ratio of the standard to the internal standard of a read result as a Y axis, carrying out linear regression analysis, and obtaining a regression equation through '1 / x2' weight; and substituting the area ratio of the oxalic acid in the sample to the internal standard peak into the standard curve equation, and calculating the concentration of the oxalic acid in the urine sample. According to the method, derivatization, solid-phase extraction, acidification and other operations are not needed, the analysis technology is greatly simplified, the detection time is shortened, and the sample stability is improved.

The utility model relates to the technical field of food detection, and discloses a sample preservation device, which comprises a preservation box and a sample tube, the inner part of the preservation box is fixedly connected with a limiting column I, the outer wall of the limiting column I is connected with a limiting round block in a sliding manner, and the outer wall of the limiting round block is rotatably connected with a sample disc; a sample groove is formed in the top end of the sample disc, the outer wall of the sample tube is slidably connected to the inner wall of the sample groove, a through groove is formed in the outer wall of the storage box, and a fixing shell is fixedly connected to the outer wall of the storage box. According to the sample taking device, the sample disc, the sample tubes, the fixed column, the gear, the rack I, the connecting block, the rack II and the reset assembly are matched with one another, so that the sample tubes can be taken out one by one in a sample taking process, and all the sample tubes do not need to be exposed outside, so that the interference of environmental change on undetected samples can be reduced to the greatest extent; the stability of the sample can be maintained, and meanwhile, the orderly detection of the sample can be facilitated.

The application provides a capability verification liquid sample stability test method and related equipment, and relates to the technical field of radionuclide analysis.The method comprises the following steps: randomly drawing a preset number of to-be-processed liquid samples from a liquid sample, determining a stability test parameter, placing the to-be-processed liquid samples into corresponding test environments for processing, obtaining to-be-tested liquid samples, and the stability test parameter at least comprising a temperature.An analysis is performed according to a standard analysis method to obtain a measurement average value of a radionuclide, the measurement average value is corrected to a preset test date according to a half-life of the radionuclide to obtain a corrected average value, and a stability conclusion of the liquid sample under the stability test parameter is obtained according to the corrected average value.The method can test factors affecting the stability of the capability verification liquid sample, avoid the influence of these factors during the analysis of the activity concentration of the radionuclide, and improve the reliability of the analysis result of the activity concentration of the radionuclide.

The utility model provides a test sample table and detection equipment, and relates to the technical field of sample detection equipment, the test sample table provided by the utility model is suitable for being installed in a detection area of a scanning electron microscope, and the test sample table comprises a base and a bearing table; the base is provided with a connecting part which is detachably connected with the scanning electron microscope; the bearing table is hinged to the base around the axis parallel to the horizontal plane and used for adjusting the included angle between the bearing face of the bearing table and the scanning electron microscope lens, then the shooting angle is adjusted, the bearing table and the base are relatively locked through a locking piece or frictional resistance, the sample stability is ensured, and the shooting efficiency is improved. And the stable detection effect of the scanning electron microscope can be ensured.

The utility model belongs to the technical field of cashmere detection, particularly relates to a cashmere fineness detection device, and provides the following scheme aiming at the problems that an existing device cannot carry out accurate detection well and cannot keep the stability of a sample in the transfer process: the cashmere fineness detection device comprises a chopping assembly, a sample containing assembly, a sliding rail and a detection microscope, according to the cashmere cutting device, the cutting assembly and the detection microscope are combined together through the arrangement of the sliding track, the sample containing assembly horizontally and linearly moves along the sliding track, shaking of the sample containing assembly is not prone to being caused, and then the cut cashmere samples cannot be displaced in the sample containing assembly; according to the cashmere sample collecting device, the cashmere sample collecting assembly is arranged to ensure that chopped cashmere samples can be always kept uniformly distributed, the sample containing assembly seals and stores the collected chopped cashmere samples, the chopped cashmere samples cannot be blown up due to external air flow, and the stability of the samples is ensured to be kept in the moving process.

The invention relates to the field of biological detection and analysis, and particularly discloses a method for detecting trace peripheral blood volatile fatty acids and fatty aldehydes based on dried blood sampling and in-situ labeling. According to the method, through synchronous dry blood sampling and in-situ labeling reaction, chemical derivatization of a target compound is completed in the sampling process, the target compound is converted into a non-volatile and chemically stable product, and the loss of the target compound is effectively avoided. Meanwhile, the adopted derivatization reagent can introduce chromophores or easy-to-ionize groups on target molecules, so that the chromatographic retention capability and the detection sensitivity of the target molecules are remarkably enhanced. According to the method, additional sample pretreatment is not needed, sampling and marking are performed synchronously, experimental errors are reduced, and sample stability and detection precision are improved. The method can be widely applied to clinical diagnosis, metabonomics research and biomedical analysis, and a novel and convenient technical scheme is provided for efficient analysis of volatile metabolites in trace blood samples.