HPCE fingerprint identification method for origin ginseng protection
A fingerprint, origin technology, applied in the field of HPCE fingerprint identification, can solve the problems of complex determination method, easy contamination of chromatographic column, not easy to clean, etc., and achieves low cost, abundant chemical information, instrument precision and sample stability. and good reproducibility
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[0046] The examples enumerated in the present invention are intended to further clarify the specific operation and application direction of the HPCE fingerprint identification method for the protection of ginseng in the original place, without any limitation to the scope of the present invention.
[0047] 1. Establish the fingerprint of ginseng in the place of origin
[0048] A. The HPCE fingerprints were made with the commercially available sun-dried and transplanted wild ginseng from Changbai Mountain as the standard product
[0049] Ginseng pretreatment: dry the ginseng medicinal material in an oven at 60°C for 4 hours, pulverize and sieve to collect fine powder below 80 mesh, weigh 3.0g, add 80% ethanol solution and heat reflux to extract twice, each time for 3 hours, combine the filtrate and naturally Filtrate, and after rotary evaporation, add water twice to make up to 10mL, place in a cool place for 12h, take the supernatant, filter with a 0.22μm cellulose membrane, and...
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