Method for separating nano-particles at water-phase density gradient centrifugation rate

A nanoparticle and gradient centrifugation technology, which is applied in solid separation, wet separation, chemical instruments and methods, etc. The effect of wide size range, good separation effect and simple process

Inactive Publication Date: 2009-10-21
BEIJING UNIV OF CHEM TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for separating nanoparticles using aqueous phase density gradient centrifugation rate, thereby overcoming some current separation techniques that require a long time, high cost, are greatly affected by sample stability and purity, and have complicated methods defects and deficiencies

Method used

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  • Method for separating nano-particles at water-phase density gradient centrifugation rate
  • Method for separating nano-particles at water-phase density gradient centrifugation rate
  • Method for separating nano-particles at water-phase density gradient centrifugation rate

Examples

Experimental program
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Effect test

Embodiment 1

[0045] (1) First, 0.1 mg of Au nanoparticle suspension containing 5nm, 10nm and 20nm was added to mercapto-polyethylene glycol 5000 aqueous solution, ultrasonically dispersed, and then concentrated to reduce its volume to 0.2ml.

[0046](2) Make a density gradient: dilute with 60% iodixanol (density 1.32 g / ml) solution by mass percentage to prepare 30%, 40%, and 50% iodixanol solutions respectively. Take 0.75ml each to prepare a density gradient solution of 30%+40%+50%+60%.

[0047] (3) Slowly add 0.2 ml of mixed Au colloidal nanoparticle solution onto the density gradient solution.

[0048] (4) High-speed centrifugation at 15°C, 50,000 rpm, and 15 minutes.

[0049] (5) Take out the centrifuged colloidal nano-dispersion from the top in equal volumes in batches (100ul / time, marked as fn in turn). Finally, Au nanoparticles with different sizes were obtained.

[0050] For separation results, see figure 2 , 3. Similar effects can be achieved by using sucrose, albumin, glycer...

Embodiment 2

[0052] (1) First, about 0.5 mg of 4 nm FeCo@C nanoparticles were ultrasonically dispersed in 5 ml of phospholipid-methylpolyethylene glycol 5000 surfactant solution with a concentration of 1 mg / ml to form 0.1 mg / ml FeCo@C colloids Nanoparticle solution.

[0053] (2) Make density gradient: dilute with 60% iodixanol (density 1.32g / ml) solution by mass percentage to prepare 10%, 20%, 30%, 40% iodixanol solutions respectively. Take 0.75ml each to prepare a density gradient solution of 10%+20%+30%+40%.

[0054] (3) Slowly add 0.2ml FeCo@C colloidal nanoparticle solution to the density gradient solution.

[0055] (4) High-speed centrifugation at 10°C, 45,000 rpm, and 210 minutes.

[0056] (5) Take out the centrifuged colloidal nano-dispersion from the top in equal volumes in batches (100ul / time, marked as fn in turn). Finally, FeCo@C nanoparticles with different sizes were obtained.

[0057] For separation results, see Figure 4 . Similar effects can be achieved by using album...

Embodiment 3

[0059] (1) First, graphite oxide is ultrasonicated in water to prepare a solution of about 2 mg / ml graphene oxide colloidal nanoparticles.

[0060] (2) Make a density gradient: first make a sucrose solution with a mass percentage of 70% (density of 1.34g / ml), then dilute the 70% sucrose solution to prepare 20%, 30%, 40%, 50%, 60% % sucrose solution. Take 0.5ml each to prepare a density gradient solution of 20%+30%+40%+50%+60%+70%.

[0061] (3) Slowly add 0.2ml graphene oxide colloidal nanoparticle solution to the density gradient solution.

[0062] (4) High-speed centrifugation at 15°C, 50,000 rpm, and 15 minutes.

[0063] (5) Take out the centrifuged colloidal nano-dispersion from the top in equal volumes in batches (100ul / time, marked as fn in turn). Finally, graphene oxide with different sizes is obtained.

[0064] For separation results, see Figure 5 . Similar effects can be achieved by using albumin, glycerol, sorbitol, meglumine, and iodixanol solutions with the s...

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Abstract

The invention relates to a method for separating nano-particles at a water-phase density gradient centrifugation rate, which mainly comprises the following steps: 1) preparing the nano-particles into a homogeneous transparent colloid nano-particle solution by means of ultrasound, stirring and the like; 2) preparing density gradient solutions with different mass percentage concentrations respectively; 3) adding certain amount of density gradient medium solutions with different concentrations into a centrifuge tube in turn to prepare a step-like or linear density gradient solution; and 4) adding the solution containing colloid nano-particles to the liquid surface of the density gradient solution slowly, and performing centrifugalization under certain condition. Because the colloid nano-particles with different sizes have different sedimentation rates in the density gradient solution and can be detained at different positions in the density gradient solution so as to achieve the effect of separation. The method has the advantages of simple method, quick separation, low cost, small influence on the stability and the purity of a sample, and the separation effect of certain section can be enhanced selectively by adjusting and controlling centrifugal parameters.

Description

technical field [0001] The invention relates to a method for separating nanoparticles, in particular to a method for separating nanoparticles in a liquid phase according to different particle sedimentation rates according to the size and properties of nanoparticles, and belongs to the technical field of inorganic advanced material separation technology. Background technique [0002] Nanotechnology developed since the 1980s has led to revolutionary advances in the fields of electronics, mechanics, physics, materials, chemistry, and biology. A series of previously unimagined excellent properties have been gradually discovered and recognized in nanomaterials. For example, carbon nanotubes (Nature, 1991, 354, 56-58) and graphene (Science, 2004, 306.666-669) have super strong mechanical properties and excellent electrical conductivity, which have aroused great interest in electronics, physics, materials and other disciplines. It is widely concerned and is considered to lead to a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B03B5/32
Inventor 孙晓明罗大超刘军枫
Owner BEIJING UNIV OF CHEM TECH
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