The invention discloses a construction method of deeply buried tunnel chamber excavation based on analysis of partition fracture evolution of surrounding rock. The construction method comprises the steps that 1, the tunnel chamber excavation is performed; 2, basic mechanical parameters of the surrounding rock is determined; 3, the analysis of the partition fracture evolution of the surrounding rock is performed, specifically, the fracture evolution analysis of the surrounding rock is carried out for an existing constructed tunnel, based on the analysis results, the number of fracture zones M and the thickness of each fracture zone on the surrounding rock of the existing constructed tunnel are determined after excavation; and 4, radial grouting reinforcement is performed, specifically, theradial grouting reinforcement of the surrounding rock of the existing constructed tunnel is carried out for multiple times along the longitudinal extension direction from back to front, every time theradial grouting reinforcement of the surrounding rock is carried out, grouting pipes are used for conducting the grouting reinforcement of the M fractured zones separately. The construction method ofthe deeply buried tunnel chamber excavation based on the analysis of partition fracture evolution of the surrounding rock is simple in steps, reasonable in design, convenient to realize and good in using effect, can perform the radial grouting reinforcement of the surrounding rock purposefully according to the analysis results of the partition fracture evolution of the surrounding rock, and can effectively ensure the stability of the surrounding rock of the tunnel chamber and the safety of tunnel chamber excavation construction.