Technique for extracting Grifola frondosa water-soluble polysaccharide

A technology of water-soluble polysaccharides and grifola frondosa, applied in the field of polysaccharide extraction technology, can solve the problems of large-scale industrial application, large amount of organic solvent, and long extraction time, etc., to maintain polysaccharide activity, mild treatment conditions, and small dosage Effect

Inactive Publication Date: 2010-06-16
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chinese patent CN1322141C discloses a method for enzymatic hydrolysis of polysaccharides from Grifola frondosa, adding cellulase, β-glucanase, pectinase and neutral protease to the fermentation broth, and after enzymolysis, deenzyme, filtration, Grifola frondosa polysaccharides were obtained through steps such as concentration, alcohol precipitation and separation, but this method has high professional requirements and is not suitable for large-scale industrial applications
"Separation, Purification and Properties of Grifola frondosa Polysaccharides by Alkaline Extraction" published by Shi Baojun, used water extraction for several times, followed by alkali extraction, acid neutralization and centrifugation for several times, alcohol precipitation, and the precipitate was washed with absolute ethanol, acetone, and ether in sequence. h 2 o 2 Pigment removal, protein removal by Sevag method, dialysis for 3 days, alcohol precipitation and drying, long extraction time, large amount of organic solvents, and chloroform used in protein removal is carcinogenic and toxic, so it is not suitable for food, medicine and health care products and other fields

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Grifola frondosa fruiting bodies were crushed, 100 g was added to 1.5 L of water, soaked dynamically at room temperature for 3 hours, then ultrasonically extracted for 20 minutes (ultrasonic power was 260 W), extracted 3 times, combined to obtain 4 L of extracts, added 5% hydrochloric acid to adjust the pH to 5.5, Add 48g of papain, keep it warm at 55°C, stir at 50 rpm for 2 hours, heat up to 90°C to inactivate the enzyme for 5 minutes after the reaction, add 53g of activated carbon for decolorization for 1 hour, and pass the decolorization solution through the hollow with a molecular weight cut-off of 3000 The fiber ultrafiltration membrane system removes macromolecular impurities, and then concentrates with a nanofiltration membrane system with a molecular weight cut-off of 100. Add 4 times the amount of 95% ethanol to the concentrated solution, and start centrifuging after precipitation, discard the supernatant, and then wash with absolute ethanol Precipitate 3 times,...

Embodiment 2

[0022] Grifola frondosa fruiting bodies were crushed, 200g was added to 3.6L of water, soaked dynamically at room temperature for 2 hours, then ultrasonically extracted for 38 minutes (ultrasonic power 360W), extracted twice, combined to obtain 7L extract, added acetic acid to adjust pH to 5, added 84g papaya Protease, keep warm at 60°C, stir at 40 rpm for 1.5 hours, heat up to 100°C to inactivate the enzyme for 5 minutes after the reaction, add 90g of activated carbon to decolorize for 2 hours, and pass the decolorized solution through a hollow fiber ultrafiltration with a molecular weight cut-off of 2000 The membrane system removes macromolecular impurities, and then concentrates with a nanofiltration membrane system with a molecular weight cut-off of 100. The concentrated solution is added with 3 times the amount of 95% ethanol. After the precipitate is precipitated, it is centrifuged, the supernatant is discarded, and the precipitate is washed twice with absolute ethanol. ,...

Embodiment 3

[0024] Grifola frondosa fruiting bodies were crushed, 500 g was added to 10 L of water, soaked dynamically at room temperature for 2.5 hours, then ultrasonically extracted for 40 minutes (ultrasonic power 450 W), extracted 3 times, combined to obtain 28 L of extracts, added 1% hydrochloric acid to adjust the pH to 6, and added 336 g Papain, keep warm at 60°C, stir at 35 rpm for 3 hours, heat up to 90°C to inactivate the enzyme for 5 minutes after the reaction, add 360g of activated carbon for decolorization for 1.5 hours, pass the decolorization solution through a hollow fiber ultra-thin with a molecular weight cut-off of 1000 The filter membrane system removes macromolecular impurities, and then concentrates with a nanofiltration membrane system with a molecular weight cut-off of 100. The concentrated solution is added with 5 times the amount of 95% ethanol. The second time, the ethanol was evaporated to dryness, and freeze-dried to finally obtain 83 g of polysaccharides with ...

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Abstract

The invention relates to a technique for extracting Grifola frondosa water-soluble polysaccharide. The technique is implemented by soaking raw material sporocarp of Grifola frondosa in water for 2-3h, ultrasonic extracting 2-4 times, regulating pH, depriving protein by papain enzymolysis process, adding activated carbon for decoloring, filtering decoloring solution by membranes for retaining and concentrating, precipitating with ethanol, and freeze drying to obtain the finished product. By adopting the technique to produce polysaccharide, cost is low, energy consumption is low, purity is high, and activity of Grifola frondosa polysaccharide is retained.

Description

Technical field: [0001] The invention relates to a polysaccharide extraction process, in particular to a method for extracting polysaccharides from Grifola frondosa fruit bodies. Background technique: [0002] Grifola frondosa (Fructificatio Polypori Frondosi), also known as chestnut mushroom, Buddha fungus and lotus fungus, is a large fungus belonging to the family Polyporaceae of the Basidiomycota subphylum Phylomycetes. The fruiting body is imbricate and clustered, the cap is fan-shaped or spoon-shaped, the surface is taupe, smoother, the surface of the hole is white to light yellow, dense and extended fungal tubes, the tube mouth is nearly round to polygonal. Light body, brittle, off-white section, uneven. Fishy smell, slightly sweet taste. The nutritional value is extremely rich, rich in a large amount of protein, fat, crude fiber, carbohydrates and a variety of beneficial minerals and vitamins. It is known as the "king of fungi and a wonderful anti-cancer flower". G...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C08B37/00
Inventor 刘东锋郭琴杨成东
Owner NANJING ZELANG MEDICAL TECH
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