Method and process for extracting animal micro-molecule polypeptides and amino acids by utilizing complex enzyme

A small molecule polypeptide and compound enzyme technology, applied in the field of protein engineering, can solve the problems of low extraction efficiency, loss of biological activity, destruction of effective components, etc., and achieve the effect of reducing production cost and improving enzymatic hydrolysis efficiency.

Inactive Publication Date: 2013-08-21
WEIHAI KANGBOER BIOLOGICAL PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional animal products are mainly processed by physical methods (such as crushing, high temperature, etc.), which not only have low extraction efficiency, but also cause a large amount of active ingredients to be destroyed and lose their biological activi

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1: Enzymatic hydrolysis of sea cucumbers with complex enzymes.

[0017] (1) Take 1 kg of fresh sea cucumber and crush it to pass through an 80-mesh sieve.

[0018] (2) Add water to make a 10% animal tissue suspension, heat at 100°C for 30 minutes, and when the temperature drops to 35°C-40°C, adjust the pH value to 8.0 with alkali, add enterokinase, stir well, and then add 1.0% animal tissue equivalent % compound enzyme I, incubated at 37°C at constant temperature, 15kHz ultrasonic enzymatic hydrolysis for 1h, adjusted to pH 7.0, adding 1.5% compound enzyme II equivalent to animal tissue, controlled temperature at 45°C, 16.5kHz ultrasonic enzymatic hydrolysis for 2h.

[0019] (3) Increase the temperature to 100°C, quickly inactivate the enzyme for 30 minutes, and then cool down naturally.

[0020] (4) Filter the enzymolysis solution through a 0.22 μm microporous membrane.

[0021] (5) Concentrate the filtrate to a relative density of 1:1.0 to obtain a tan conce...

Embodiment 2

[0022] Example 2: Enzymatic hydrolysis of fresh oyster meat with complex enzymes.

[0023] (1) Take 1 kg of oyster meat and crush it so that it can pass through a 100-mesh sieve.

[0024] (2) Add water to make a 25% suspension, increase the temperature to 35°C-40°C, adjust the pH value to 8.5 with sodium carbonate, add compound pancreatin I equivalent to 0.8% of animal tissue after stirring, and keep the temperature at 40°C Incubation, 14.5kHz ultrasonic enzymatic hydrolysis for 1.5h, adjust the pH value to 7.5, add compound enzyme II equivalent to 1.0% of animal tissue, control the temperature at 40°C, and 15kHz ultrasonic enzymatic hydrolysis for 4h.

[0025] (3) Increase the temperature to 100°C, quickly inactivate the enzyme for 20 minutes, and then cool down naturally.

[0026] (4) Filter the enzymolysis solution through a 0.22 μm microporous membrane.

[0027] (5) Concentrate the filtrate to a relative density of 1:1.0 to obtain a concentrated solution.

Embodiment 3

[0028] Example 3: Enzymatic hydrolysis of abalone with complex enzymes.

[0029] (1) Take 1 kg of abalone meat, crush it so that it can pass through a 100-mesh sieve.

[0030] (2) Add water to make a 20% suspension, increase the temperature to 35°C to 40°C, adjust the pH value to 8.5 with sodium bicarbonate, add compound enzyme I equivalent to 1.1% of animal tissue after stirring, and heat at 35°C Incubate at constant temperature, 16kHz ultrasonic enzymatic hydrolysis for 1.0h, adjust pH to 6.5, add compound enzyme II equivalent to 1.2% of animal tissue, temperature control at 37°C, 17kHz ultrasonic enzymatic hydrolysis for 2h.

[0031] (3) Increase the temperature to 95°C, quickly inactivate the enzyme for 30 minutes, and then cool down naturally.

[0032] (4) Filter the enzymolysis solution through a 0.22 μm microporous membrane.

[0033] (5) Concentrate the filtrate to a relative density of 1:1.0 to obtain a concentrated solution.

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PUM

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Abstract

The invention discloses a method and process for extracting animal micro-molecule polypeptides and amino acids by utilizing a complex enzyme. The method comprises the following steps: (1) crushing animal tissues so that the animal tissues can pass through a sieve of 80-200 meshes, and adding water to prepare a suspension with the concentration of 8-30 percent; (2) raising the temperature to be 35-40 DEG C, regulating the pH value to be 8.0-9.0 by using sodium carbonate, adding a complex enzyme I accounting for 0.5-2.0 percent of the animal tissues, performing ultrasonic enzymolysis at 14-16 kHz for 1-2 hours so that the proteins between cells are subjected to full enzymolysis, regulating the pH value to be 6.5-7.5, adding a complex enzyme II accounting for 0.5-3.0 percent of the animal tissues, fully and uniformly stirring, controlling the temperature of the water bath to be 37-45 DEG C, and performing ultrasonic enzymolysis at 15-17 kHz for 1-6 hours; (3) raising the temperature of the enzymolysis solution to be 90-100 DEG C to inactivate the enzyme for 20-30 minutes after the enzymolysis is finished; (4) filtering the solution subjected to enzymolysis through a 0.22mu m microfiltration membrane; and (5) concentrating the filtrate to the relative density of 1:1.0-1:1.2 to prepare the concentrated solution, wherein the complex enzyme II consists of Alcalase, Flavourzyme, Protamex, neutral protease and papain. According to the method, the enzymolysis efficiency can be improved, and the production cost can be reduced.

Description

technical field [0001] The invention belongs to the technical field of protein engineering, in particular to a method and process for extracting animal small molecule polypeptides and amino acids by using compound enzymes. Background technique [0002] Compared with plants, the composition and ratio of various proteins in animals are closer to those of humans, so the protein components in animals have higher nutritional value for humans, especially some small molecular polypeptides and peptides with specific physiological functions in animals. Amino acids (biologically active substances) play an important role in regulating human metabolism. Traditional animal products are mainly processed by physical methods (such as crushing, high temperature, etc.), which not only have low extraction efficiency, but also cause a large amount of active ingredients to be destroyed and lose their biological activity. Although bio-enzyme technology is used in individual processing processes,...

Claims

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Application Information

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IPC IPC(8): C12P21/06C12P13/00
Inventor 于国友王佃亮于丽萍王思远
Owner WEIHAI KANGBOER BIOLOGICAL PHARMA
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