Method for distilling myricetin from plant

A technology of myricetin and red bayberry, applied in the direction of organic chemistry, can solve the problems of difficulty in purifying myricetin, low content, high cost, etc., and achieve the effects of convenient product separation and purification, simple preparation process, and low production cost

Inactive Publication Date: 2006-12-06
GUANGZHOU HANFANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Because the content of myricetin in bayberry bark and Yao nationality vine tea is relatively low, it is susceptible to the interference of other components, especially the interference of dihydromyricetin, which brings certain difficulties for the purification of myricetin. difficulty
At present, the method for extracting myricetin from bibliographical reports has: in Journal of Basic Chinese Medicine, 1999,13 (46): 6 discloses the method for extracting and separating myricetin from vine tea of ​​the Yao nationality, and it is degreased with sherwood oil Extract with ethanol afterward, and then separate with silica gel chromatography column, this method cost is extremely high, is only suitable for laboratory research, is not suitable for large-scale batch production; The disclosed method of extracting and separating myricetin from vine tea of ​​the Yao nationality in , it is after extracting with water, repeatedly recrystallizes the method with solvent repeatedly, and the yield of this method is relatively low, only 0.51% (according to crude drug weight Count) Therefore, there is no report of a kind of more effective, suitable for large-scale batch production to extract the process technology of myricetin

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  • Method for distilling myricetin from plant

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1, get 1.5kg red bayberry bark and be ground into coarse powder, add 95% ethanol 1500ml heating reflux, each 2 hours, extract altogether 3 times, merge filtrate, filtrate is concentrated to dry, extract is refluxed with ethyl acetate 1500ml 2 times, each time for 1 hour, filter, combine the filtrates, recover the solvent to about 750ml, let stand for 8 hours, filter, and recover the filtrate to dryness under reduced pressure to obtain the crude myricetin. Add 70% ethanol to dissolve the crude myricetin, then add water to adjust the ethanol concentration to 35%, filter, and the filtrate is adsorbed with an adsorption column filled with 30-60 mesh polyamide, and after adsorption, it is carried out with 35% ethanol solution For elution, the eluate was discarded, followed by elution with 65% ethanol solution, and the eluate was collected. After ethanol was recovered from the eluate under reduced pressure, high-purity myricetin was precipitated, filtered, and the ...

Embodiment 2

[0019] Embodiment 2, get 2kg Yao Nationality vine tea stem and leaf and pulverize into coarse powder, add 2000ml of 85% ethanol and heat to reflux, each time 2 hours, extract altogether 3 times, combine filtrate, filtrate is concentrated to dry, extract with ethyl acetate 2000ml Reflux twice, each time for 1 hour, filter, combine the filtrates, recover the solvent to about 1000ml, let stand overnight, filter, and recover the filtrate to dryness under reduced pressure to obtain the crude myricetin. Add 80% ethanol to dissolve the crude myricetin, then add water to adjust the ethanol concentration to 30%, filter, and the filtrate is adsorbed with an adsorption column filled with polyamide, after adsorption, elute with 30% ethanol, discard Remove the eluate, continue to elute with 70% ethanol solution, and collect the eluate. After ethanol was recovered from the eluent under reduced pressure, high-purity myricetin was precipitated, filtered, and the precipitate was collected and ...

Embodiment 3

[0020] Embodiment 3, get 1.5kg red bayberry bark and be ground into coarse powder, add 70% ethanol 1500ml heating reflux, each 2 hours, extract altogether 3 times, merge filtrate, filtrate is concentrated to dry, extract is refluxed with ethyl acetate 1500ml 2 times, each time for 1 hour, filter, combine the filtrates, recover the solvent to about 750ml, let stand for 10 hours, filter, and recover the filtrate to dryness under reduced pressure to obtain the crude myricetin. Add 80% ethanol to dissolve the myricetin crude product, add water to adjust the ethanol concentration to 35%, filter, and the filtrate is adsorbed with an adsorption column filled with D101 macroporous adsorption resin. After adsorption, wash with 38% ethanol solution Remove, discard the eluate, continue to elute with 75% ethanol solution, and collect the eluate. After the eluate was decompressed and recovered the alcohol solution, high-purity myricetin was precipitated, filtered, and the precipitate was c...

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Abstract

This invention discloses a method for extracting myricetin from Mirica rubra bark or Ampelopsis grossedentata. The method comprises: (1) extracting Mirica rubra bark or Ampelopsis grossedentata with ethanol; (2) extracting with a medium polarity organic solvent; (3) adsorbing crude myricetin by polyamide or polar macroporous resin, eluting and separating to obtain the final product. The method has such advantages as high yield, simple separation and high product purity (greater than or equal to 80%), and is suitable for mass product.

Description

Technical field [0001] The invention relates to a method for extracting myricetin from plant raw materials, which belongs to the technology of preparing flavonoid compound myricetin from natural plants, specifically a method for extracting and separating myricetin from bark of bayberry and vine tea plants of the Yao nationality . Background technique [0002] Myricetin is the bark of Myrica rubra (Lour.) Sieb. et Zucc. or the bark of Myrica rubra (Lour.) Sieb. et Zucc. -Mazz) stems and leaves (also known as Yao vine tea) contain myricetin flavonoids, which have significant physiological activities such as protecting the liver, reducing enzymes, reducing jaundice, improving immune function, lowering blood sugar, and anti-PAF. In Basic Chinese Medicine Journal, 1999, 13(46): 6, the structure of myricetin has been disclosed as follows: [0003] [0004] Myricetin is 3, 5, 7, 3', 4', 5'-hexahydroxyflavone with a phenolic acid structure, easily soluble in methanol, ethanol, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/30
Inventor 李吉来钟鸣刘菊妍
Owner GUANGZHOU HANFANG PHARMA
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