Method for composite enzymolysis-assisted extraction of active ingredient from propolis

Abstract

The invention provides a method for composite enzymolysis-assisted extraction of an active ingredient from propolis, and belongs to the extraction technology of propolis. The method comprises the following steps of: selecting high-quality rough propolis as a raw material; removing impurities, refrigerating, crushing and then homogenously mixing a certain amount of enzymolysis agent; filling the mixed raw material on a biological enzymolysis bed; performing biological enzymolysis by adjusting parameters such as enzymolysis temperature, humidity, pH, time and the like; extracting flavonoid from the propolis in a classified way in an extraction tank by controlling factors such as alcoholic strength, solid-to-liquid ratio, temperature, pressure, time and the like; and performing reduced pressure distillation by controlling concentration temperature and vacuum degree to remove impurities and moisture. The method has the obvious advantages that: 1, a propolis enzymolysis technology and a propolis alcohol extraction technology are integrally applied to production practice, and the method has a simple process and an accurate technological parameter range and is easy in realization of industrialization; 2, the contents of flavone aglycones such as myricetin, quercetin, apigenin, pinocembrin and the like in extracted active ingredient of the propolis are increased; and 3, the antioxidative activity of a propolis enzymolysis product can be obviously improved.

Description

technical field [0001] The invention relates to a method for extracting effective components in propolis, in particular to a method for assisting extraction of effective components in propolis by using compound enzymolysis. Background technique [0002] Propolis is the resin and juice collected by bees from the bark or shoots of collagenous plants such as poplar, birch, willow and coniferous trees, and mixed with the secretion of the bee's own throat glands. Flavonoids are the main nutritional components of propolis, and propolis flavonoids have a wide range of biological activities, such as anti-oxidation, anti-tumor, anti-inflammation, and regulation of immune cells. The propolis paste obtained after propolis is purified generally contains 30% flavonoids, which contains more flavonoid glycosides. The research results show that the biological activity of flavone aglycone in scavenging oxygen free radicals in the human body is significantly better than that of flavone glyco...

AI Technical Summary

Problems solved by technology

At present, at home and abroad, most of the flavone glycosides are hydrolyzed by chemical methods, and the flavones are changed through chemical derivatization reactions such as etherification, esterification, and acylation to change the characteristics of flavones, such as improving fat solubility, but these reaction processes often shield flavonoids. The main functional group - phenolic hydroxyl group, has adverse effects on antioxidant activity, and is prone to environmental pollution during processing

[0003] At present, the extraction methods of propolis industrialization are mainly ethanol extraction and supercritical CO2 extraction. Most of the propolis flavonoids extracted by these two extraction methods are flavone glycosides, and no modification of their molecular structure is carried out. Therefore, through the existing The nutrients of propolis extracted by technology are still flavonoid glycosides with low utilization rate in human body