Liver soothing and depression relieving type weight reducing tea for regulating AMPK / SREBP-1c pathway and preparation method thereof

Abstract

The invention discloses liver-soothing and depression-relieving type medicinal and edible weight-reducing tea based on AMPK / SREBP-1c pathway regulation and control and a preparation method of the weight-reducing tea. A targeted extraction technology is introduced and comprises the steps of step-by-step extraction, embedding purification, liquid phase preparation and the like, key components, such as hawthorn flavone, nuciferine and the like, acting on an SREBP-1c pathway are accurately extracted, the key components and medicinal and edible components such as ginseng, poria cocos and the like in a formula have a synergistic effect, AMPK phosphorylation is effectively activated, SREBP-1c nuclear translocation is inhibited, and the SREBP-1c protein is prepared. Deeper lipid metabolism regulation and control and liver-intestinal axis balance are realized. Cell and animal experiments show that the tea can significantly reduce accumulation of triglyceride and has no side effects of drugs.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese medicine beverage technology, specifically to a medicinal and edible light body tea formula and preparation method that achieves the dual effects of soothing the liver and relieving depression and reducing fat by regulating the AMPK / SREBP-1c metabolic pathway, suitable for people with obesity, metabolic syndrome and emotional obesity. Background Technology

[0002] Most commercially available weight-loss teas focus on promoting bowel movements and urination or physically inhibiting fat absorption. While they may achieve short-term weight loss, they are prone to side effects such as metabolic rebound and endocrine disorders, and neglect the core role of emotional stress in obesity formation. Modern medical research shows that obesity in people with liver stagnation constitution is closely related to abnormalities in the AMPK / SREBP-1c pathway. AMPK (adenosine monophosphate-activated protein kinase) plays a key role in energy metabolism, reducing fatty acid production and accelerating its oxidation; while SREBP1c (sterol regulatory element-binding protein 1c) is a major regulator of fatty acid metabolism, participating in processes such as lipogenesis. In obesity, the reduction of SREBP1c is associated with genomic instability and aging of adipocytes, while the activation of AMPK helps improve lipid metabolism. In addition, gut microbiota dysbiosis can lead to weight gain and abnormal energy metabolism, affecting the liver-gut axis function, and liver-gut axis dysfunction is closely related to metabolic diseases such as non-alcoholic fatty liver disease. Although existing weight-loss teas contain ingredients such as tea polyphenols, caffeine, and plant extracts, and have certain weight-loss effects, they fail to provide specific interventions for obesity caused by emotional stress. This invention aims to provide a liver-soothing and mood-regulating medicinal and food-based weight loss tea based on the AMPK / SREBP-1c pathway regulation. By scientifically combining various medicinal and food-based ingredients, it achieves the dual effects of soothing the liver and relieving mood and reducing fat, and provides a unique solution for obesity caused by emotional stress. Summary of the Invention

[0003] (I) Formulation Composition (by weight)

[0004] Active ingredients: Ginseng 8-9%, Poria cocos 11-12%, White hyacinth bean 8-9%, Amomum villosum 4-5%, Platycodon grandiflorus 8-9%, Coix seed 11-12%, Tangerine peel 11-12%, Roasted hawthorn 26-27%, Lotus leaf 1-2%, Rose 3-4%

[0005] Additional ingredient: 2-4% honey (to adjust the taste and enhance the stability of the ingredients)

[0006] Collaboration mechanism:

[0007] AMPK activation: Roasted hawthorn contains flavonoids, which can inhibit SREBP-1c nuclear translocation 49 by activating AMPK phosphorylation; lotus leaf alkaloids synergistically enhance the activity of the AMPK pathway.

[0008] Targeted extraction: Through stepwise extraction, encapsulation purification, and liquid phase preparation, key components acting on the SREBP-1c pathway are precisely extracted and purified, improving the efficacy and stability of the product.

[0009] Soothe the liver and regulate qi: Rose petals and dried tangerine peel regulate liver qi stagnation and reduce stress-induced lipid deposition.

[0010] Strengthening the spleen and removing dampness: Poria cocos and Coix seed reduce endoplasmic reticulum stress by regulating intestinal flora and indirectly inhibit SREBP-1c expression.

[0011] (II) Preparation Method

[0012] (1) Purchase the required raw materials according to the preset formula requirements, and remove impurities and dry them thoroughly.

[0013] (2) After crushing the charred hawthorn, use 70% ethanol solution as the extraction solvent. Add the solvent at a ratio of 10:1 (solvent to charred hawthorn raw material mass). Reflux extraction is carried out at 65℃ for 2 hours. Repeat the extraction process twice and combine the three extracts to obtain charred hawthorn extract.

[0014] (3) After crushing the lotus leaves, water is used as the extraction solvent. The solvent is added in the same ratio of 10:1 to the mass of the lotus leaf raw material. The decoction is carried out at 85°C for 1 hour. The decoction process is repeated twice. The decoctions from the three decoctions are combined to obtain the lotus leaf decoction.

[0015] (4) Filter the hawthorn extract and lotus leaf decoction obtained in steps (2) and (3) respectively to remove the residue.

[0016] (5) Hawthorn flavonoids (derived from charred hawthorn extract) and β-cyclodextrin were mixed at a mass ratio of 1:2, heated to 50-60℃, stirred at a constant temperature for 2 hours, and the encapsulated mixture was spray-dried to obtain the purified components.

[0017] (6) Mix lotus leaf alkaloid (derived from lotus leaf decoction) with β-cyclodextrin at a mass ratio of 1:3 and perform encapsulation treatment under the same conditions as in step (5).

[0018] (7) Accurately weigh the purified hawthorn flavonoids, lotus leaf alkaloids and other medicinal and edible raw materials (poria, white hyacinth bean, amomum villosum, platycodon grandiflorum, coix seed, tangerine peel and rose) according to the formula ratio, add an appropriate amount of water, and stir thoroughly to dissolve all the components evenly and form a stable liquid mixture.

[0019] (8) Heat the liquid mixture to boiling and cook over medium heat for 5 minutes. Add cardamom, turn to high heat and boil for 5 minutes, then filter to remove residue to obtain liquid 1.

[0020] (9) After soaking the ginseng for 30 minutes, bring it to a boil, then turn to a simmer and continue to cook for 15 minutes to obtain liquid 2.

[0021] (10) Mix liquid 1 and liquid 2 evenly in a ratio of 5:1. During the mixing process, gradually add honey (the amount added is 3% of the total mass of the mixture), maintain the temperature of the mixture at 55°C, and continue stirring until the honey is completely dissolved.

[0022] (11) The flavored light tea was subjected to high-temperature sterilization at 121°C for 15 minutes. The temperature of the sterilized light tea was reduced to 42°C using rapid cooling technology.

[0023] (12) The cooled light tea is filled into pre-sterilized glass bottles and stored at 4°C.

[0024] (III) Beneficial Effects

[0025] The slimming tea of ​​the present invention has the following advantages:

[0026] (1) Scientific formulation: It uses a variety of medicinal and edible raw materials, which are combined with each other. From the perspective of soothing the liver and strengthening the spleen, it regulates the body's metabolic function, reduces fat synthesis and promotes fat decomposition by regulating the AMPK / SREBP-1c pathway, thereby achieving the purpose of weight loss.

[0027] (2) Reasonable preparation method: Different medicinal materials are prepared by different cooking methods. Advanced processes such as stepwise extraction, encapsulation purification, and liquid phase preparation are introduced to fully extract the effective components and improve the effectiveness and stability of the product.

[0028] (3) High safety: All raw materials are food and medicine homologous substances, and no chemically synthesized weight loss ingredients are added. They have no obvious side effects on the human body and are suitable for long-term consumption.

[0029] (4) Good taste: Adding honey to flavor the tea improves its taste and makes it more acceptable to consumers. Attached Figure Description

[0030] Figure 1 The figure shows the changes in AML-12 cell viability. In the figure, a and b represent the effects of oleic acid and light tea on AML-12 cell viability, respectively. (n=6). “ns” indicates no statistical significance.

[0031] Figure 2 The figure shows the intracellular triglyceride content in each experimental group. In the figure, "***" indicates a highly significant difference compared with the NC group (P<0.001); "#" indicates a significant difference compared with the OA group (P<0.05).

[0032] Figure 3Figure 1 shows the rat body weight. In the figure, figure a represents the rat body weight before modeling, and figure b represents the rat body weight after the intervention with Qingti Tea. "ns" indicates no statistical significance; "***" indicates a highly significant difference (P<0.001).

[0033] Specific Implementation Cases

[0034] The present invention will be further described below with reference to embodiments.

[0035] Example 1:

[0036] A medicinal and edible slimming tea that achieves dual effects of soothing the liver and relieving depression and reducing fat by regulating the AMPK / SREBP-1c metabolic pathway. The formula is as follows: ginseng 8%, poria cocos 11%, white hyacinth bean 8%, amomum villosum 5%, platycodon grandiflorus 8%, coix seed 11%, tangerine peel 11%, charred hawthorn 27%, lotus leaf 2%, rose petals 4%, and honey 3%.

[0037] The specific preparation process is as follows:

[0038] (1) Purchase the required raw materials according to the preset formula requirements, and remove impurities and dry them thoroughly.

[0039] (2) After crushing the charred hawthorn, use 70% ethanol solution as the extraction solvent. Add the solvent at a ratio of 10:1 (solvent to charred hawthorn raw material mass). Reflux extraction is carried out at 65℃ for 2 hours. Repeat the extraction process twice and combine the three extracts to obtain charred hawthorn extract.

[0040] (3) After crushing the lotus leaves, water is used as the extraction solvent. The solvent is added in the same ratio of 10:1 to the mass of the lotus leaf raw material. The decoction is carried out at 85°C for 1 hour. The decoction process is repeated twice. The decoctions from the three decoctions are combined to obtain the lotus leaf decoction.

[0041] (4) Filter the hawthorn extract and lotus leaf decoction obtained in steps (2) and (3) respectively to remove the residue.

[0042] (5) Hawthorn flavonoids (derived from charred hawthorn extract) and β-cyclodextrin were mixed at a mass ratio of 1:2, heated to 50-60℃, stirred at a constant temperature for 2 hours, and the encapsulated mixture was spray-dried to obtain the purified components.

[0043] (6) Mix lotus leaf alkaloid (derived from lotus leaf decoction) with β-cyclodextrin at a mass ratio of 1:3 and perform encapsulation treatment under the same conditions as in step (5).

[0044] (7) Accurately weigh the purified hawthorn flavonoids, lotus leaf alkaloids and other medicinal and edible raw materials (poria, white hyacinth bean, amomum villosum, platycodon grandiflorum, coix seed, tangerine peel and rose) according to the formula ratio, add an appropriate amount of water, and stir thoroughly to dissolve all the components evenly and form a stable liquid mixture.

[0045] (8) Heat the liquid mixture to boiling and cook over medium heat for 5 minutes. Add cardamom, turn to high heat and boil for 5 minutes, then filter to remove residue to obtain liquid 1.

[0046] (9) After soaking the ginseng for 30 minutes, bring it to a boil, then turn to a simmer and continue to cook for 15 minutes to obtain liquid 2.

[0047] (10) Mix liquid 1 and liquid 2 evenly in a ratio of 5:1. During the mixing process, gradually add honey (the amount added is 3% of the total mass of the mixture), maintain the temperature of the mixture at 55°C, and continue stirring until the honey is completely dissolved.

[0048] (11) The flavored light tea was subjected to high-temperature sterilization at 121°C for 15 minutes. The temperature of the sterilized light tea was reduced to 42°C using rapid cooling technology.

[0049] (12) The cooled light tea is filled into pre-sterilized glass bottles and stored at 4°C.

[0050] The slimming tea prepared through the above methods has a mellow taste and good effects on soothing the liver and spleen, and slimming.

[0051] Example 2:

[0052] A medicinal and edible slimming tea that achieves dual effects of soothing the liver and relieving depression and reducing fat by regulating the AMPK / SREBP-1c metabolic pathway has the following formula: ginseng 9%, poria cocos 12%, white hyacinth bean 9%, amomum villosum 4%, platycodon grandiflorus 9%, coix seed 12%, tangerine peel 12%, charred hawthorn 26%, lotus leaf 1%, rose petals 3%, and honey 2%.

[0053] The preparation method of the above-mentioned slimming tea is the same as that in Example 1.

[0054] The light-weight tea drinks from Examples 1 and 2 were subjected to the following functional tests:

[0055] 1. Cell experiments

[0056] (1) Cytotoxicity test

[0057] AML-12 cells were resuscitated and cultured in a DMEM / F-12 incubator at 37°C with 5% CO2. Cells were passaged when they reached 70%-80% confluence. The experiment was divided into a control group (NC), an oleic acid group (OA, 0.4 mmol / L), a low-dose tea group (JF-L, 0.4 mmol / L OA + 25 μmol / L tea), and a high-dose tea group (JF-H, 0.4 mmol / L OA + 50 μmol / L tea). Cells were seeded in 96-well plates, treated with the candidate reagent for 24 h, and cell viability was measured using the MTT assay.

[0058] The results are as follows Figure 1 As shown, after 24 hours of treatment, the MTT assay revealed no statistically significant difference in AML-12 cell viability between the different concentrations of the light tea and OA groups and the control group (NC) (P > 0.05). This indicates that within the selected concentration range, neither the light tea nor OA significantly affected the survival of AML-12 cells.

[0059] Conclusion: The results of this study indicate that the light tea beverage has relative safety for AML-12 cells at the tested concentration.

[0060] (2) Triglyceride (TG) determination

[0061] A control group, an oleic acid (OA) group, and low-dose (25 μmol / L) and high-dose (50 μmol / L) light body tea were established. Based on the MTT assay results, the effect of the tea on reducing triglyceride (TG) accumulation in AML-12 cells was investigated. After establishing a high-lipidemia model in AML-12 cells, the cells were treated with 25 μmol / L and 50 μmol / L light body tea for 16 hours, respectively.

[0062] Experimental results are as follows Figure 2 As shown, compared with the control group, the intracellular TG value in the OA group was significantly increased, successfully simulating a high-fat environment. Under the treatment of 25 μmol / L and 50 μmol / L light tea, the intracellular TG value in AML-12 cells was significantly lower than that in the group with only OA added (P < 0.05), indicating that the light tea can significantly reduce TG accumulation in AML-12 cells under a high-fat environment.

[0063] Conclusion: The light-weight tea can significantly reduce TG accumulation in AML-12 cells under a high-fat environment, and has potential weight loss function.

[0064] 3. Animal experiments

[0065] (1) Fifty SPF-grade SD rats were selected and, after one week of acclimatization, were randomly divided into (n=10 / group): blank control group (NC, normal diet + saline), model group (HFD, high-fat diet + saline), positive control group (OL, high-fat diet + orlistat 0.048g / (kg·d)), low-dose slimming tea group (JF-L, high-fat diet + slimming tea extract 1g / kg·d), and high-dose slimming tea group (JF-H, high-fat diet + slimming tea extract 4g / kg·d). The blank control group was given normal diet and saline by gavage, while the other groups were fed high-fat diet and were given the corresponding drugs or saline by gavage, respectively, for 8 weeks. The high-fat diet formula was 69.5% normal diet + 15% lard + 15% sucrose + 0.5% bile salts.

[0066] Experimental results are as follows Figure 3 As shown, there was no statistically significant difference in mouse weight among the treatment groups at the beginning of the experiment. After 8 weeks of continuous treatment, the body weight of mice in the HFD treatment group increased significantly, indicating that the diet-induced obesity model was successfully established. Under OL, JF-L, and JF-H treatments, the body weight of mice was significantly lower than that of the HFD treatment group (P < 0.05). There was no significant difference between the OL treatment and the JF-L and JF-H treatment groups, indicating that the slimming tea can significantly alleviate the induction of weight gain in mice by a high-fat diet, and has no drug side effects.

[0067] Conclusion: The slimming tea can significantly reduce the body weight and triglyceride accumulation in obese mice, and has potential weight loss function.

[0068] (2) Record weight weekly and calculate Lee's index, liver index and epididymal fat index. The experimental results are shown in Table 1.

[0069] Table 1

[0070] Group Lee's Index Liver index (% wet weight) Epididymal fat index (%) NC 298±12 3.1±0.3 1.2±0.1

[0071] Continued from Table 1

[0072] Group Lee's Index Liver index (% wet weight) Epididymal fat index (%) HFD 345±15 5.5±0.5 4.2±0.4 OL 305±14** 3.6±0.3** 2.3±0.2** JF-L 328±16* 4.1±0.4* 3.0±0.3* JF-H 302±13** 3.5±0.3** 2.0±0.2**

[0073] Note: Compared with the HFD group, "*" indicates a significant difference (P<0.05), and "**" indicates a highly significant difference (P<0.01).

[0074] (3) After the intervention, fasting for 12 hours was required. Blood was collected from the heart after anesthesia to separate serum, and the serum TG, TC, LDL-C and HDL-C levels were measured. The experimental results are shown in Table 2.

[0075] Table 2

[0076]

[0077] Note: Compared with the HFD group, "*" indicates a significant difference (P<0.05), and "**" indicates a highly significant difference (P<0.01).

[0078] (4) The expression levels of AMPKα1 and SREBP-1c genes in liver tissue were detected by real-time quantitative PCR. The experimental results are shown in Table 3.

[0079] Table 3

[0080] Group AMPKα1 SREBP-1c FAS ACC PPARα CPT-1 NC 1.00±0.10 1.00±0.12 1.00±0.09 1.00±0.11 1.00±0.08 1.00±0.10 HFD 0.62±0.08** 2.41±0.25** 3.15±0.30** 2.85±0.28** 0.42±0.05** 0.55±0.06** OL 1.35±0.12* 1.65±0.18** 2.10±0.20** 1.95±0.19** 0.78±0.07* 0.92±0.09* JF-L 1.52±0.15** 1.32±0.14* 1.80±0.17** 1.60±0.15** 1.25±0.12* 1.30±0.13** JF-H 1.88±0.18** 0.85±0.09** 1.25±0.12** 1.10±0.11** 1.90±0.18** 1.75±0.16**

[0081] Note: Compared with the HFD group, "*" indicates a significant difference (P<0.05), and "**" indicates a highly significant difference (P<0.01).

[0082] The weight-loss tea formula developed in this study demonstrated a significant intervention effect on a high-fat diet-induced obesity model in rats, with experimental data showing a 16.1% reduction in body weight and a 44.0% decrease in epididymal fat index. Its mechanism of action exhibits dual regulatory characteristics: by activating the AMPKα1 phosphorylation signaling pathway, it significantly inhibits the expression of the key lipid synthesis regulator SREBP-1c and its downstream target genes FAS and ACC; simultaneously, it upregulates PPARα / CPT-1 to promote mitochondrial β-oxidation and accelerate lipid breakdown. This weight-loss tea is safe and did not cause side effects such as diarrhea. This study provides a natural solution for obesity intervention based on energy metabolism regulation and develops a candidate product that meets the dual requirements of functional food safety and efficacy, demonstrating significant translational value.

[0083] The safety and efficacy of this invention were verified through cell and animal experiments. Cell experiments showed that this slimming tea significantly activated AMPK phosphorylation, inhibited SREBP-1c nuclear translocation, reduced fat synthesis, and promoted fat breakdown. Animal experiments showed that this slimming tea significantly reduced the body weight and triglyceride accumulation in obese mice, with no drug side effects.

[0084] The preferred embodiments of the present invention have been described in detail above, but are not intended to limit the concept and scope of the invention. Within the scope of the technical concept of the present invention, various simple modifications can be made to the technical solutions of the present invention, including combinations of various technical features in any other suitable manner. These simple modifications and combinations, as long as they do not depart from the inventive concept or exceed the scope defined in these claims, should all fall within the protection scope of the present invention.

Claims

1. A medicinal and edible slimming tea with liver-soothing, mood-lifting, and fat-reducing effects, characterized in that: Ginseng 5-10 parts, Poria cocos 10-15 parts, white hyacinth bean 5-10 parts, Amomum villosum 2-5 parts, Platycodon grandiflorus 5-10 parts, Coix seed 10-15 parts, dried tangerine peel 10-15 parts, charred hawthorn 25-30 parts, lotus leaf 1-5 parts, rose petals 2-5 parts, and honey 2-5 parts.

2. The medicinal and edible slimming tea with liver-soothing, depression-relieving, and fat-reducing effects according to claim 1, characterized in that: Ginseng 8-9 parts, Poria cocos 11-12 parts, white hyacinth bean 8-9 parts, Amomum villosum 4-5 parts, Platycodon grandiflorus 8-9 parts, Coix seed 11-12 parts, dried tangerine peel 11-12 parts, charred hawthorn 26-27 parts, lotus leaf 1-2 parts, rose petals 3-4 parts, honey 2-4 parts.

3. The medicinal and edible slimming tea with liver-soothing, depression-relieving, and fat-reducing effects according to claim 1, characterized in that: Ginseng 8 parts, Poria cocos 11 parts, white hyacinth bean 8 parts, Amomum villosum 5 parts, Platycodon grandiflorus 8 parts, Coix lacryma-jobi 11 parts, dried tangerine peel 11 parts, charred hawthorn 27 parts, lotus leaf 2 parts, rose petals 4 parts, and honey 3 parts.

4. A medicinal and edible slimming tea with liver-soothing, depression-relieving, and fat-reducing effects according to claim 1, characterized in that: Ginseng 9 parts, Poria cocos 12 parts, white hyacinth bean 9 parts, Amomum villosum 4 parts, Platycodon grandiflorus 9 parts, Coix lacryma-jobi 12 parts, dried tangerine peel 12 parts, charred hawthorn 26 parts, lotus leaf 1 part, rose petals 3 parts, honey 2 parts.

5. A medicinal and edible slimming tea with liver-soothing, depression-relieving, and fat-reducing effects according to claims 1-4, characterized in that: The dosage form of the light tea is tea bags, powder, granules, or tea pills.

6. A method for preparing a medicinal and edible slimming tea as described in any one of claims 1-4, characterized in that, Includes the following steps: Step S1: Select medicinal and edible raw materials until they are free of mold and impurities and are thoroughly dried; Step S2: Crush the charred hawthorn into 40 mesh, add 70% ethanol solution at a material-to-liquid ratio of 1:10, reflux extract at 65℃ for 2 hours, repeat the extraction 3 times, combine the extracts and concentrate under reduced pressure to a relative density of 1.15-1.20 (measured at 60℃) to obtain crude hawthorn flavonoid extract; Step S3: Crush the lotus leaves to 60 mesh, add deionized water at a material-to-liquid ratio of 1:15, decoct at 85°C for 1 hour, repeat the decoction twice, combine the decoctions and concentrate to 1 / 5 of the original volume to obtain lotus leaf alkaloid concentrate. Step S4: Mix the crude extract of hawthorn flavonoids with β-cyclodextrin at a mass ratio of 1:2, stir at 50℃ for 2 h, and spray dry (inlet temperature 160℃, outlet temperature 80℃) to obtain encapsulated flavonoid micro powder. Step S5: Mix lotus leaf alkaloid concentrate with β-cyclodextrin at a mass ratio of 1:3, stir at 60℃ for 2 h, and spray dry (inlet temperature 150℃, outlet temperature 75℃) to obtain lotus leaf alkaloid micro powder. Step S6: Mix the encapsulated flavonoid powder, the encapsulated lotus leaf alkaloid powder and other food and medicinal materials (Poria cocos, Amomum villosum, etc.) according to the formula ratio, add deionized water (material-liquid ratio 1:8), stir and dissolve at 50°C for 30 min to form a homogeneous liquid phase mixture. Step S7: Heat the liquid mixture to boiling, maintain medium heat for 5 minutes, add cardamom and boil over high heat for 5 minutes, then filter to obtain liquid 1. Step S8: Soak the ginseng slices for 30 minutes, bring to a boil over high heat, then simmer over low heat for 15 minutes, and filter to obtain liquid 2. Step S9: Mix liquid 1 and liquid 2 in a ratio of 5:1, add honey (3% of the total mass), and stir continuously at 55°C until completely dissolved; Step S10: Sterilize the mixture at 121°C for 15 min, then use a jacketed circulating cooling system (cooling rate ≥8°C / min) to reduce the temperature to 42°C, fill it into pre-sterilized glass bottles, and store it at 4°C away from light.

7. The preparation method according to claim 6, characterized in that, In step S2, the volume fraction of the ethanol solution extracted from the charred hawthorn is 70 ± 2%, and the reflux temperature is controlled at 65 ± 1℃.

8. The preparation method according to claim 6, characterized in that, In step S3, the ratio of lotus leaf decoction to liquid is 1:15 (w / v), and the decoction time error does not exceed ± 5 min.

9. The preparation method according to claim 6, characterized in that, In step S4, the inlet air temperature of the spray dryer is 160±5℃, the outlet air temperature is 80±3℃, and the material residence time is ≤ 15 s.

10. The preparation method according to claim 6, characterized in that, In step S9, the amount of honey added is 3 ± 0.5% of the total mass of the mixture, and the dissolution temperature is controlled at 55 ± 2℃.

11. The preparation method according to claim 6, characterized in that, In step S10, the high-temperature sterilization is performed using a pulsed vacuum sterilizer with sterilization parameters of 121°C, 15 min, and 0.12 MPa.

12. The use of a medicinal and edible herbal tea as described in any one of claims 1-4 in the preparation of health products or foods for weight loss, lipid reduction, or liver and spleen regulation.

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