A kind of preparation method of jellyfish antihypertensive peptide

A technology of antihypertensive peptides and jellyfish, which is applied in the field of preparation of polypeptide mixtures, can solve the problems of poor activity preservation, low purity, and low yield of antihypertensive peptides, and achieve the effects of reduced production costs, high extraction rates, and simple operations

Active Publication Date: 2011-12-21
吴川市天然食品加工有限公司
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  • Application Information

AI Technical Summary

Problems solved by technology

[0006] There have been reports at home and abroad to extract antihypertensive peptides from jellyfish or other marine animal and plant resources, but the obtained products are basically bold proteins or centrifuged supernatants, and the partial yield of antihypertensive peptides is low, low in purity, and poorly preserved in activity. There are many deficiencies as a raw material for antihypertensive products

Method used

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  • A kind of preparation method of jellyfish antihypertensive peptide

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Experimental program
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Effect test

Embodiment 1

[0026] Removal of various metal ions in jellyfish: First, use an ultrapure water machine to extract deionized water, which requires an impedance value of not less than 10. Mix the two according to the mass ratio of salted jellyfish to ultrapure water of 1:2, and stir for 30 Minutes; then extract deionized water from the ultrapure water machine, requiring an impedance value of not less than 15, mix the two according to the mass ratio of salted jellyfish to ultrapure water of 1:2, and stir for 30 minutes; The machine extracts deionized water, and the impedance value is required to be not less than 18. According to the mass ratio of salted jellyfish to ultrapure water of 1:1, mix the two and stir for 15 minutes.

[0027] Drain the jellyfish after removing the metal ions, put it into a tissue meat grinder and grind it; put the ground jellyfish into a homogenizer, and homogenize it at 5000r / min for 10 minutes.

[0028] Dilute the homogenized jellyfish slurry 100 times with ultrapur...

Embodiment 2

[0032] First, pre-treat the salted jellyfish to remove various metal ions in the jellyfish, and extract deionized water from the ultrapure water machine. The impedance value is required to be not less than 10. According to the quality of salted jellyfish to ultrapure water is 1:2 Mix the two and stir for 30 minutes; then extract deionized water from the ultrapure water machine, requiring an impedance value of not less than 15, mix the two according to the mass ratio of salted jellyfish to ultrapure water of 1:2, and stir 30 minutes; finally extract deionized water from the ultrapure water machine, the impedance value is required to be no less than 18, mix the two according to the mass ratio of salted jellyfish to ultrapure water of 1:1, and stir for 15 minutes.

[0033] Take out the jellyfish and drain the water, put it into the tissue meat grinder and grind it; put the ground jellyfish into the homogenizer, and homogenize it at 5000r / min for 10 minutes; the jellyfish slurry af...

Embodiment 3

[0038] First, pre-treat the salted jellyfish to remove various metal ions in the jellyfish, and extract deionized water from the ultrapure water machine. The impedance value is required to be not less than 10. According to the quality of salted jellyfish to ultrapure water is 1:2 Mix the two and stir for 30 minutes; then extract deionized water from the ultrapure water machine, requiring an impedance value of not less than 15, mix the two according to the mass ratio of salted jellyfish to ultrapure water of 1:2, and stir 30 minutes; finally extract deionized water from the ultrapure water machine, the impedance value is required to be no less than 18, mix the two according to the mass ratio of salted jellyfish to ultrapure water of 1:1, and stir for 15 minutes.

[0039] Take out the jellyfish and drain the water, put it into a tissue meat grinder and grind it; put the ground jellyfish into a homogenizer, and homogenize it at 5000r / min for 10 minutes.

[0040]Dilute the homogen...

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Abstract

The invention provides a preparation method for jelly fish neurotensin, which comprises the following steps of: carrying out metallic ion removal treatment on jelly fish; sequentially carrying out steps of homogenating, enzymolysis, enzyme deactivating, concentration, ultrafiltration, freeze drying and the like; and finally obtaining purified jelly fish neurotensin powder. The method combines the modern enzyme engineering technology, provides a novel efficient production path for the jelly fish neurotensin, improves the utilization efficiency of jelly fish resources and has the characteristics that the operation is simple, the extraction rate is high, and the product activity preservation is good. The production cost of the jelly fish neurotensin is reduced. The rich protein resources of the jelly fish are sufficiently utilized, and various metallic ions in jelly fish bodies are basically and clearly removed through the metallic ion removal treatment, so the subsequent enzymolysis and application are safer. Results of jelly fish neurotensin antihypertensive activity experiments adopting spontaneously hypertensive rat (SHR) models show that the group of the preparation method obviously differs from the blank control group. The jelly fish neurotensin prepared by the preparation method has the antihypertensive activity similar to positive control captopril.

Description

technical field [0001] The invention relates to a preparation method of a polypeptide mixture, in particular to a preparation method of a jellyfish antihypertensive peptide. Background technique [0002] Hypertension is a disease that seriously harms human health, and it is also the main factor that causes cardiovascular diseases. Its harm is to cause damage to important organs such as the brain, heart, and kidneys. Stroke and coronary heart disease are the most serious complications of hypertension. Data show that the average incidence rate of the world is 10% to 20%, and the number of deaths due to hypertension reaches 12 million every year. At present, there are more than 120 million hypertensive patients in my country. Therefore, the treatment and prevention of hypertension is a very important topic in today's society. [0003] At present, medical research on hypertension mainly focuses on ACE inhibitors (ACEI). Currently, chemically synthesized ACEIs such as Captopr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34
Inventor 林靖善胡光耀
Owner 吴川市天然食品加工有限公司
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