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Detecting method for captopril through light emitting silver clusters synthesized by G insertion sequences

A technology of fluorescence detection and sequence, which is applied in the directions of measurement devices, fluorescence/phosphorescence, material excitation analysis, etc., can solve the problems of low sensitivity, high cost, environmental pollution, etc., and achieve the effect of high sensitivity

Active Publication Date: 2019-01-04
JIANGNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, the commonly used captopril detection methods include gas chromatography, liquid phase-mass spectrometry, high-performance liquid chromatography, etc. These methods are time-consuming and costly, and require professional experimenters in specialized laboratories. Carry out, or the sensitivity is low, and it is easy to cause environmental pollution

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  • Detecting method for captopril through light emitting silver clusters synthesized by G insertion sequences
  • Detecting method for captopril through light emitting silver clusters synthesized by G insertion sequences
  • Detecting method for captopril through light emitting silver clusters synthesized by G insertion sequences

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Experimental program
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Effect test

Embodiment 1

[0030] Prepare the DNA-AgNCs solution using the following conditions:

[0031] (1) Preparation of DNA-AgNCs solution: Take 20 μL of phosphate buffer, add DNA aqueous solution with a final concentration of 5 μM, and then add 2.0 μL of AgNO with a concentration of 3.0 mM 3 Aqueous solution, stirred for 30s, kept at 4°C for 20min, then added 2.0μL fresh NaBH 4 aqueous solution and vigorously stirred for 1 min, and the mixture was stored at room temperature in the dark for 9 h to prepare a DNA-AgNCs nanocluster solution;

[0032] (2) Screening of DNA sequences: select different C-rich base DNA sequences in Table 1, synthesize DNA-AgNCs according to step (1), measure its fluorescence intensity at the maximum emission wavelength (670nm), the results are as follows figure 1 As shown, the fluorescence intensities corresponding to the C4A and C6G sequences are relatively strong, 26730 and 27394 respectively. It can be seen that the C6G sequence has the highest intensity, so the G inse...

Embodiment 2

[0045] Add different concentrations of captopril to the 2.5 μM C6-5G-AgNCs nanocluster solution prepared in Example 1, the total reaction volume is 400 μL, and the final concentrations of captopril are 0, 0.05, 0.1, 0.2 . Figure 6 As shown, there is a good linear relationship in the captopril concentration range (0~1μg / mL), and the linear equation is y=-42774.68x+45953.36(R 2 = 0.9446). The detection sensitivity can reach 0.05μg / mL, and the detection linear range is 0.05μg / mL~5μg / mL.

Embodiment 3

[0047] The total reaction volume was 400 μL, and the concentration of C6-5G-AgNCs was 2.5 μM. Captopril at a concentration of 1 μg / mL, starch at 50 μg / mL, and Dextrin, 10 μg / mL maltose, 20 μg / mL gelatin, 10 μg / mL sucrose, 50 μg / mL lactose, 10 μg / mL glucose, and as a blank control, after fully reacting for 20 minutes, measure at the emission wavelength of 670nm Fluorescence intensity to determine the selectivity of C6G5-AgNCs to captopril detection, the results are as follows Figure 7 As shown, only the C6-5G-AgNCs added with captopril was significantly quenched in the fluorescence. The fluorescence intensity of the blank control group was 72251, that of the captopril group was 7737, that of the starch group was 63195, and that of the lactose group was 50311. and in Figure 7 In (b), it can be directly observed that the brightness of the sample added with captopril is much weaker than that of the other eight, indicating that the selectivity of C6G5-AgNCs for the detection of ...

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Abstract

The invention discloses a detecting method for captopril through light emitting silver clusters synthesized by G insertion sequences, and belongs to the fields of food and medicine detection. A seriesof regular C-base-enriched DNA sequences are deigned for synthesizing nanometer silver clusters, the G insertion sequence with the best fluorescent brightness is selected for synthesizing the silverclusters, and a method for detecting captopril is built; a sample to be tested possibly containing captopril is added into a silver nanometer cluster solution, after a mixing reaction, and detection of captropril in the sample to be detected is achieved by observing the fluorescent strength of the formed mixed reaction system under the maximum excitation wavelength. The silver nanometer cluster synthesized by the DNA sequences is applied for detecting captopril, the detection linear range is 0.05-5 microgram / mL, the sensitivity can reach 0.05 microgram / mL, and the method has the advantages ofbeing easy, convenient and rapid to use, low in cost, high in sensitivity and the like, and can be applied to detection of captopril in healthy products of healthcare products, food, medicine productsand other healthy products.

Description

technical field [0001] The invention relates to a method for detecting captopril by a luminescent silver cluster synthesized by a G insertion sequence, and belongs to the field of food and drug detection. Background technique [0002] Silver nanoclusters (AgNCs), as a nanomaterial with photoluminescent properties and catalytic activity, are widely used in analytical chemistry, biomedicine, and cell imaging. DNA-AgNCs synthesized using natural biological DNA as a template have fluorescence properties, and the fluorescence intensity is related to the gene arrangement and structure in the DNA sequence. The study found that Ag + Can selectively coordinate C bases and form stable C-Ag + -C complex, G (guanine)-rich sequences can increase the fluorescence intensity of DNA-AgNCs in the detection of nucleic acids, ATP, thrombin and heavy metals, and some DNA-capped AgNCs with high fluorescence intensity are usually rich in C and The sequence of G bases, so creatively design a ser...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432
Inventor 郭亚辉张文雅张瑶钱和成玉梁谢云飞于航姚卫蓉
Owner JIANGNAN UNIV
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