130 results about "Amyloid like" patented technology

The invention relates to a deep learning-based early-stage amyloidosis nephropathy identification and diagnosis method, belongs to the technical field of amyloidosis nephropathy identification, and solves the problem that early-stage amyloidosis cannot be accurately identified in the prior art. The method comprises the following steps: acquiring kidney pathological section images of different individuals to construct a training sample set; constructing a link model comprising a first region segmentation model, a second region segmentation model and a third region segmentation model; the first region segmentation model is used for segmenting a cortex region in the pathological image; the second region segmentation model is used for segmenting a glomerular region; the third region segmentation model is used for identifying amyloidosis of a glomerular region; training the link model based on the training sample set to obtain a trained link model; and inputting a to-be-identified kidney pathological section image into the trained link model to identify whether amyloidosis exists or not. And efficient and accurate early-stage amyloidosis identification is realized.

The invention provides application of a biomarker combination for early screening and diagnosis of transthyretin cardiac amyloidosis (ATTR-CA) high-risk groups in preparation of a product for early screening and diagnosis of the ATTR-CA high-risk groups. In general, one or more biomarkers, such as total prealbumin (total PA), transthyretin (TTR) tetramer, misfolded protein, protein misfolding rate, and soluble growth stimulus expression gene 2 protein (sST2). In ATTR-CA high-risk groups and non-high-risk groups, the biomarkers show significant difference, which prompts that the biomarkers are related to ATTR-CA. When a plurality of biomarkers are combined for use, the biomarkers have relatively high sensitivity and specificity in early screening and diagnosis of ATTR-CA high-risk groups, have relatively high diagnosis efficiency, and provide a new thought and strategy for early diagnosis and treatment of ATTR-CA.

Compounds and compositions comprising compounds that modulate histone acyltransferase (HAT). Methods of treating neurodegenerative disorders, conditions associated with amyloid-beta peptide deposit accumulation, Tau protein levels, and / or alpha-synuclein accumulation, and cancer by administering to a subject a compound that modulates HAT.

This invention relates to isolated polypeptides that are analogs of human amylin. The disclosed amylin analog polypeptides have beneficial physicochemical properties relative to endogenous amylin, such as longer elimination half-lives (t1 / 2) and improved solubility and thermal stability. This invention also relates to methods of using presently disclosed amylin analog polypeptides in a variety of therapeutic indications, as well as methods of producing the same. The disclosed amylin analog polypeptides are particularly useful in methods of treating metabolic diseases or disorders, such as types 1 and 2 diabetes, and providing weight loss.

To provide a novel amyloid beta (A beta) binding molecule, especially an amyloid beta antibody or an antigen-binding fragment thereof, and / or use of them.SOLUTION: There is provided an amyloid beta binding antibody including: VH-CDR1 including a specific amino acid sequence, VH-CDR2 including a specific amino acid sequence, VH-CDR3 including a specific amino acid sequence, VL-CDR1 including a specific amino acid sequence, VL-CDR2 including a specific amino acid sequence, and VL-CDR3 including a specific amino acid sequence; or an antigen-binding fragment thereof. In one embodiment, independently of an amyloid beta peptide or a three-dimensional structure states of a seed, an amyloid beta binding antibody or an antigen binding fragment thereof selectively binds to an amyloid beta peptide or seed in a solution, or captures the amyloid beta peptide or seed.SELECTED DRAWING: None

One aspect relates to a method for providing information for predicting a risk group for developing Alzheimer's disease dementia or a risk group for early onset of Alzheimer's symptoms, or a risk group for developing amnestic mild cognitive impairment and / or a positron emission tomography (PET)-positive risk group for amyloid β deposition, based on a European population-East Asian data. According to one aspect, the method makes it possible to accurately predict a risk group for developing Alzheimer's disease dementia or a risk group for early onset of Alzheimer's symptoms, or a risk group for developing amnestic mild cognitive impairment and / or a positron emission tomography (PET)-positive risk group for amyloid β deposition by using only at least 12 single-nucleotide polymorphisms, and the ability to predict the risk groups is further enhanced when up to and at most 80 additional single-nucleotide polymorphisms are used. In addition, the method further includes identifying age, sex, years of education, and APOE genotype as indicators, and thus the ability to predict the risk groups is further enhanced, and the risk groups can be predicted at an early stage with high accuracy.

The invention discloses a nano flocculant as well as a preparation method and application thereof. The preparation method comprises the following steps: preparing a protein amyloid fiber solution; adding a zirconium salt compound, and mixing to obtain a zirconium ion-loaded protein amyloid fiber composite solution; and mixing with a poly dimethyl diallyl ammonium chloride solution. The nano flocculant provided by the invention can be stored for a long time at normal temperature, is simple and convenient to use, is suitable for double improvement of sedimentation efficiency and dephosphorization effect in municipal and industrial sewage treatment processes, and has wide engineering application prospects and environmental friendliness.

The invention discloses a preparation method and application of a compound with emulsifying and antibacterial dual functions, and belongs to the technical field of food active packaging materials. The compound is prepared through a two-step method, a synergistic antibacterial mechanism is formed by means of the hydrophobic binding effect of the amyloid fiber and the cationic electrostatic interaction of the chitosan, and meanwhile excellent emulsion stability and antibacterial activity are achieved through the hydrogen bond-electrostatic interaction of the amyloid fiber and the chitosan. When the compound is applied to preparation of the polysaccharide-based composite film, the mechanical property of the film can be remarkably improved. The raw materials are all natural degradable components, the preparation process is simple, and the obtained composite film can efficiently prolong the shelf life of aquatic products, conforms to the green packaging development trend and has important application value in the field of food active packaging.

The invention discloses a method for improving the starch digestibility inhibition effect of a spray drying method by using high amylose, and belongs to the technical field of food processing. According to the invention, highland barley glutelin, low-amylose-content corn starch, normal-amylose-content corn starch and high-amylose-content corn starch are selected, and a spray drying technology is used to simulate an embedding structure of protein in a natural grain structure on the surface of starch particles. The method comprises the following steps: dissolving protein in a 0.02% NaOH solution, then adding corn starch with different amylose contents, stirring for 30 minutes, then carrying out spray drying treatment, controlling the addition amount of the protein to be 10% of the total mass of the starch, setting the inlet temperature of a spray dryer to be 170 DEG C, setting the outlet temperature to be 80 DEG C, and controlling the feeding speed of a peristaltic pump to be 35 mL / min; the appearance of the prepared starch sample is similar to the spatial structure in highland barley grains, and the digestibility of the highland barley grains is reduced.

The invention discloses application of a miR-3960 inhibitor, and belongs to the technical field of biological medicines. The invention aims to find and discover miRNA markers related to AD diagnosis and treatment. Researches on AD pathogenesis discover that expression of the miR-3960 is significantly increased in AD patients, and further cell experiments discover that inhibition of the expression of the miR-3960 significantly improves the influence of A beta1-42 on cell viability, apoptosis, ROS and APP proteins, and the miR-3960 has a significant therapeutic effect on AD. A beta1-42 is a core component of amyloid plaque in the brain of an AD patient, and is widely applied to construction of AD cell models and animal models so as to research pathogenesis and drug evaluation of AD. The miR-3960 inhibitor is used for preparing a medicine for treating the Alzheimer's disease, and the nucleotide sequence of the miR-3960 inhibitor is CCCCCGCCUCCGCCGCCGCC.

Provided herein is a method of detecting an amyloid peptide in a patient sample, including exposing the patient sample to a binding reagent in the presence of an assay binding buffer, thereby immunoprecipitating the amyloid peptide; washing the immunoprecipitated amyloid peptide; eluting the washed, immunoprecipitated amyloid peptide, thereby generating free amyloid peptide; and analyzing the free amyloid peptide with a mass spectrometer.

The application discloses a micro electrochemical sensor for detecting amyloid polypeptide A beta 1‑42 and a preparation method and application thereof. The preparation method comprises the following steps: taking an acupuncture needle as a bare electrode, sequentially modifying tetrachloroauric acid trihydrate-gold nanoparticles, single-wall carbon nanotubes, hemin, amyloid polypeptide A beta 1‑42 template molecules, mixed polydopamine and ion liquid to form an imprinting polymer, and then eluting to obtain the micro electrochemical sensor ANME / / hemin / / SMIP electrode. The micro electrochemical sensor can be used for high-sensitivity detection of amyloid polypeptide A beta 1‑42 , and realizes specific detection of amyloid polypeptide A beta 1‑42 .

Disclosed is a method for measuring an Aβ peptide, comprising: mixing a blood sample comprising an Aβ peptide and an antibody that specifically binds to the Aβ peptide to form a complex of the Aβ peptide and the antibody; releasing the AP peptide from the complex with a basic solution comprising an organic solvent; and measuring the released Aβ peptide by mass spectrometry.

A peptide-based vaccine for use in the treatment or prevention of amyloid-to-thyroid amyloidosis (ATTR) is provided.

This invention provides material compositions and / or methods suitable for the prevention and / or treatment of protein depletion (protein deficiency), comprising material compositions that preserve the natural function of peptides / proteins while limiting and / or preventing the formation and / or aggregation of said peptides / proteins into amyloid protein. It also provides material compositions and formulations for enhancing peptide / protein solubility, stability, cycle time, receptor interaction, brain permeability, CSF half-life, and promoting peptide / protein synthesis and purification.

The present invention discloses a method for monitoring the amyloid fibrillization process of lysozyme based on cluster luminescence. The method utilizes the property that lysozyme itself can emit fluorescence in an aggregated state, and the intensity of the fluorescence changes with the change of the aggregation state of lysozyme, thereby achieving the purpose of monitoring the amyloid fibrillization process of lysozyme. The method only needs to perform fluorescence detection on the fluorescence of the protein solution that can form amyloid fiber aggregates, without the introduction of exogenous fluorescent probes. The method is simple and has lower cost. The method can fully present the overall process of amyloid fibrillization and has the advantage of high sensitivity, especially high sensitivity to the early stages of amyloid fibrillization.

Provided herein are compositions comprising a nucleic acid encoding an amyloid-beta precursor protein and / or a nucleic acid encoding premature protein-1, cells comprising these compositions, animals comprising these compositions, methods of making these cells and animals, methods of modeling Alzheimer's disease, and methods of preparing such cells and animals. A method of assessing a treatment candidate for treating or ameliorating a symptom or phenotype of Alzheimer's disease, and a method of assessing a treatment candidate for preventing or preventing a symptom or phenotype of Alzheimer's disease.

The present application relates to the use of vascular BACE1 as a therapeutic target for diseases related to cerebral small vessel injury. Specifically, it relates to the use of a substance that reduces the expression level of the beta-secretase (BACE1) gene and / or an inhibitor of the enzymatic activity of BACE1 protein in the preparation of a drug for treating or alleviating cerebrovascular disease, wherein the disease related to cerebral small vessel injury is preferably Alzheimer's disease, amyloid cerebral small vessel disease, and vascular dementia. The inventors have found that an increase in the content of BACE1 can cleave the tight junction protein Occludin, resulting in the degradation and loss of tight junction proteins between vascular endothelial cells, causing the structure and function of the blood-brain barrier to be damaged, ultimately leading to cerebral microbleeds and cognitive dysfunction and other pathological phenotypes of diseases related to cerebral small vessel injury; inhibition of the enzymatic activity of BACE1 can improve these pathological phenotypes of diseases related to cerebral small vessel injury.

The application belongs to the technical field of composite materials, and particularly discloses a fiber hybrid membrane for synchronously removing radioactive cesium and strontium as well as a preparation method and application thereof. The preparation method comprises the following steps: adjusting the pH of a beta-lactoglobulin solution, centrifuging, collecting the supernatant, filtering, dialyzing, freeze-drying, and obtaining beta-lactoglobulin monomers; dissolving the beta-lactoglobulin monomers in ultrapure water, adjusting the pH, and water-bath heating to obtain an amyloid fiber solution; adding potassium titanate to the amyloid fiber solution, adjusting the pH, and obtaining amyloid fiber loaded with titanium dioxide; and mixing the amyloid fiber loaded with titanium dioxide with activated carbon to obtain an amyloid fiber hybrid membrane. The fiber hybrid membrane for synchronously removing radioactive cesium and strontium and the preparation method and application thereof have high membrane flux, excellent adsorption selectivity and cycle performance, can accurately adsorb radioactive cesium and strontium in a complex seawater system, and do not produce secondary waste in the treatment process, and are low in price.

This invention relates to a method for preparing a plasma sample containing amyloid β-peptides for mass spectrometry analysis, comprising the following steps: a) contacting the plasma sample with a denaturing agent; b) performing a first solid-phase extraction step on the solution obtained in step a) to recover a first eluent; c) performing a second solid-phase extraction step on the first eluent obtained in step b) to recover a second eluent; and d) drying the second eluent obtained in step c) and processing it for mass spectrometry analysis, wherein the solution obtained in step d) contains intact amyloid β-peptides Aβ40 and Aβ42.

This invention discloses a nano-flocculator, its preparation method, and its applications. The preparation method includes: preparing a protein amyloid cellulose solution; adding a zirconium salt compound and mixing to obtain a zirconium-loaded protein amyloid cellulose composite solution; and mixing with a polydimethyldiallyl ammonium chloride solution. The nano-flocculator provided by this invention can be stored for a long time at room temperature, is easy to use, and is suitable for improving both sedimentation efficiency and phosphorus removal in municipal and industrial wastewater treatment processes, showing broad engineering application prospects and environmental friendliness.

The use of hydronium ion-conducting amyloid fibers or of wires comprising a plurality of hydronium ion-conducting amyloid fibers for generating electrical energy from a humid surrounding atmosphere. The invention also relates to a device comprising such fibers or wires.

The invention relates to a method of enriching a sample for amyloid β peptides, comprising (i) contacting the sample with a solid support having thereon an immobilised anti-amyloid β peptide antibody;

The present disclosure is directed to improved peptide immunogen constructs targeting portions of the Tau protein for the treatment and / or prevention of tauopathies. The present disclosure is also directed to improved peptide immunogen constructs targeting portions of the amyloid beta protein for the treatment and / or prevention of tauopathies. The present disclosure is also directed to compositions and kits containing the peptide immunogen constructs, in particular compositions and kits comprising combinations of various peptide immunogen constructs. Methods of making and using the peptide immunogen constructs, and antibodies produced by the peptide immunogen constructs, are also provided herein. In some aspects, the disclosure is directed to compositions comprising peptide immunogen constructs based on portions of the Tau protein and peptide immunogen constructs based on portions of the amyloid beta protein, and use of these peptide immunogen constructs and compositions in therapeutic methods.

The invention belongs to the technical field of composite materials, and particularly discloses a fiber hybrid membrane capable of synchronously removing radioactive cesium and strontium and a preparation method and application thereof, the preparation method comprises the following steps: adjusting the pH value of a beta-lactoglobulin solution, centrifuging, collecting supernate, filtering, dialyzing and freeze-drying to obtain a beta-lactoglobulin monomer; the preparation method comprises the following steps: dissolving beta-lactoglobulin monomers in ultrapure water, adjusting the pH value, and heating in a water bath to obtain an amyloid fiber solution; adding potassium titanate into the amyloid fiber solution, and adjusting the pH value to obtain amyloid fiber loaded titanium dioxide; and mixing the amyloid fiber loaded titanium dioxide with activated carbon to obtain the amyloid fiber hybrid membrane. According to the fiber hybrid membrane capable of synchronously removing radioactive cesium and strontium and the preparation method and application thereof, the hybrid membrane has high membrane flux, excellent adsorption selectivity and cycle performance, can accurately adsorb radioactive cesium and strontium in a huge and jumbled seawater system, does not generate secondary waste in the treatment process, and is low in price.

The invention pertains to a method for reducing amyloid plaques in the brain of a human suffering from a disease characterized by Aβ plaques in the brain, e.g., Alzheimer's disease. The invention also relates to a method for preventing or reducing amyloid plaques in the brain of a primary prevention subject. Certain aspects of the invention involve methods, doses, or dosing regimens that decrease the risk, frequency, severity, or occurrence of ARIA in subjects.

To provide a new method for forming amyloid fibrils, by which the amyloid fibrils can simply be formed compared with a conventional method.SOLUTION: A method of forming amyloid fibrils from an amyloid fibril forming protein, the method comprising: flowing a fluid comprising the amyloid fibril forming protein into a fluid tube; and conveying the fluid by means for moving the fluid by applying pressure and vacuum to the fluid through the fluid tube by progressively compressing the fluid tube in one direction, thereby forming the amyloid fibrils from the amyloid fibril forming protein.SELECTED DRAWING: Figure 1

Aggregation-induced emission luminogens useful for imaging β-amyloid peptide and aggregates thereof, pharmaceutical compositions comprising the same, and methods of use and preparation thereof.

Adhesive formulations comprising bovine serum albumin, b-lactoglobulin, and / or a-lactalbumin can be applied and cured underwater to form a strong adhesive. Curing can be accomplished using heat or via a chemical denaturing component.

The present disclosure relates to heterogeneous cell compositions derived from canine or feline uterine tissue and methods of producing and use thereof. In some aspects, the heterogeneous cell compositions comprise a mixture of mesenchymal progenitor cells and epithelial progenitor cells. In some aspects, the heterogeneous cell compositions are used as an autologous or allogeneic treatment for the treatment of diseases such as chronic kidney disease, atopic dermatitis, immune mediated arthritis, hepatitis, liver disease, inflammatory bowel disease, osteoarthritis, intravertebral disc disease, keratoconjunctivitis sicca (dry eye), pancreatitis, fibrosis, sclerosis, amyloidosis, immune mediated polyarthritis or wounds in canines and felines.