Method for preparing dipeptidyl peptidase IV (DPP-IV) inhibitory peptide through using hairtail

Abstract

The invention discloses a method for preparing a DPP-IV inhibitory peptide through using hairtail. The method is characterized in that the method comprises the following steps: adding water to hairtail, and rubbing to form a uniform fish flesh pulp; putting the fish flesh pulp in an enzymatic hydrolysis tank, adding endo protease, and carrying out stirring hydrolysis for 4-12h to obtain a hydrolysate; heating to 95-100DEG C, and maintaining the temperature unchanged for 10-15min for enzyme killing; cooling the hydrolysate to 40-60DEG C, adding exoproteinase, and carrying out stirring hydrolysis for 2-8h; heating to 95-100DEG C, and maintaining the temperature unchanged for 10-15min for enzyme killing; centrifuging the obtained enzyme-killed hydrolysate, and taking the obtained supernatant; adding the supernatant to an ultrafilter membrane separator, adjusting the pressure and the interception molecular weight of the ultrafilter membrane to 0.05-0.1MPa and 3000Da respectively, and collecting an ultrafilter membrane permeate liquid; and separating and purifying the DPP-IV inhibitory peptide through adopting an affinity chromatography process. The method has the advantages of simple technology, low production cost and short production period.

Description

technical field

[0001] The present invention relates to the field of biotechnology, and specifically refers to a method for preparing dipeptidyl peptidase IV (DPP-IV) inhibitory peptides by using hairtail as raw material, hydrolyzing hairtail protein with compound enzymes, and using techniques such as ultrafiltration and affinity chromatography . technical background

[0002] Hairtail, also known as saury and octopus, is a species of hairtail of the order Perciformes. It is a kind of economic fish with high production in my country's coastal areas. It is delicious and is favored by consumers at home and abroad. Hairtail protein is a complete protein, containing eight essential amino acids, calcium, iron, phosphorus, magnesium, zinc, selenium and other trace elements and multivitamins, rich in nutrition. Traditional Chinese medicine believes that hairtail has the effects of harmonizing the appetite, warming the stomach, nourishing the deficiency, moisturizing the skin and be...